Wakopak® Wakobeads-T Series3

These HPLC columns filled with styrene-divinyl benzene-based porous spherical gel beads are designed for analysis of sugars, sugar alcohols and organic acids. The columns can analyze sugars ranging from monosaccharides to polysaccharides according to molecular size. Columns with some packing materials come also in economy and small sizes.

Features

Physical properties and specifications
Column T-130 T-130-E T-131 T-131-E T-131-S T-330 T-331 T-240 T-550 T-132-E T-100-S
Exclusion limit molecular weight 1×103 1×103 3×103 3×103 2×104 5×105 - -

Theoretical plate number

8,000 12,000 4,500 7,000 1,000 4,000 5,000 8,000 8,000 16,000 5,000

Upper limit flow rate (mL/min)

2.3 1.2 2.0 1.3 2.0 0.8 2.0 2.0 2.5 1.0 2.0

Upper limit pressure (kg/cm2)

15 40 7 40 40 20 20 10 13 40 40

Upper limit temperature (°C)

85 85 85 85 85 85 85 85 85 85 80
Elution time of sugars and sugar alcohols in the case of use of Wakobeads-T Series
Sample 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16
Digitoxose Xylose Arabinose Ribose Lixose Xylitol Rhamnose Fucose Glucose Sorbose Galactose Mannose Fructose Inositol Mannitol Sorbitol
M W 148 150 152 164 180 182
Column T130 14.9 15.0 16.5 18.2 15.6 15.8 14.2 16.1 14.0 14.5 15.1 15.2 15.4 15.8 14.2 15.0
T131 17.6 16.4 18.7 31.3 19.1 25.3 16.7 18.3 14.9 16.8 16.8 18.0 19.4 19.5 21.4 25.8
T331 19.9 19.1 22.2 24.6 20.5 22.9 19.8 23.6 19.0 18.7 21.6 22.1 21.6 26.5 20.7 22.7
Sample 17 18 19 20 21 22 23 24 25 26 27 28 29 30
Glucoheptose Trehalose Maltose Cellobiose Sucrose Melibiose Gentiobiose Isomaltose Lactose Multitose Melezitose Raffinose Maltotriose Stachyose
M W 210 342 344 504 666
Column T130 13.8 11.2 11.6 11.2 11.3 11.6 10.9 11.5 11.6 11.7 9.6 9.8 10.1 8.7
T131 20.2 12.6 13.2 12.6 12.3 13.4 12.4 12.9 13.6 17.0 11.3 11.6 12.6 10.8
T331 19.3 16.4 17.4 17.2 16.2 19.7 17.0 18.3 19.7 20.0 14.4 16.4 16.4 16.2

※Eluent: Water (1×10-4N NaOH for T131) Flow rate 1.0 mL/min Column temperature: 60°C
The elution time (min) slightly varies depending on the column and analysis conditions. The data should be regarded only as a guide.

Reference standards
Product Name Use Size Package Size Wako Cat. No.
Wakopak® WB-T-130

for Separation and Analysis of sugars and sugar alcohols

10 mm × 300 mm(T) 1 pc. -
Wakopak® WB-T-130-E Economy column 7.8 mm × 300 mm(W) 1 pc. 234-62083
Wakopak® WB-T-131 10 mm × 300 mm(T) 1 pc. -
Wakopak® WB-T-131-E Economy column 7.8 mm × 300 mm(W) 1 pc. 231-62093
Wakopak® WB-T-131-S Small size column 8.0 mm × 100 mm(W) 1 pc. -
Wakopak® WB-T-330 10 mm × 300 mm(T) 1 pc. -
Wakopak® WB-T-331 10 mm × 300 mm(T) 1 pc. -
Wakopak® WB-T-240 10 mm × 300 mm(T) 1 pc. -
Wakopak® WB-T-550 10 mm × 300 mm(T) 1 pc. -
Wakopak® WB-T-132-E Economy column For analysis of organic acids 7.8 mm × 300 (W) 1 pc. 234-62103
Wakopak® WB-T-100-S Small size column

For simultaneous analysis of monosaccharides and sugar alcohols

6.0 mm × 150 (W) 1 pc. -

※ (T): Tosoh type (W): Waters type