These are solid-phase extraction columns filled with a divinyl benzene-polymethacrylate resin-based filler. Presep® PFC-II is filled with a new polymer developed for the purpose of highly efficient recovery of wide range of PFCs. These columns are used as pretreatment columns for PFCs, such as perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS).

1. Trace PFCs in water can be highly efficiently recovered. Presep® PFC-II can recover 16 PFCs.
2. 16 components can be analyzed simultaneously and quickly by combining with Wakopak® Wakosil-II 3C18 RS.
3. Blank caused by solvent can be reduced by combining with a solvent for PFOS or PFOA analysis.

● PFCs reference standard is added to purified water, and the recovery is measured. Analyzed components: 16 components


Column conditioning:
Column:Presep® PFC-II
① CH3OH with 0.1% NH4OH10 mL
② CH3OH 5 mL
③ H2O 5 mL *1

Column:Presep®-C PFC
① CH3OH  10 mL
② H2O    5 mL *1

Collection/concentration operation
Water sample (additive recovery experiment: Addition of standard to 1 L of purified water)
 ↓
Feed of water into column (flow velocity: 10 to 20 mL/min)
 ↓
Drying (air purging
*2 or nitrogen gas purging)
 ↓
Elution: Amount of solvent: 2 mL
*3
 ↓(Elution solvent)Presep® PFC-II: CH3OH with 0.1 % NH4OH 2 mL
 ↓Presep®-C PFC: CH3OH 2 mL
LC/MS/MS measurement

*1: Ion exchange water fed into unused Presep® cartridge which has been exposed to recovery experiment is used. Ultrapure water for PFOs/PFOA analysis (Wako Catalog No.212-01363) can be used.
*2: Contamination from air can be prevented by connecting unused Presep® in the previous stage of the column which has collected the sample and sucking air.
*3: The eluate is concentrated by nitrogen gas purging according to the measurement sensitivity, and the quantity is measured.

Sample
Name
Presep®
PFC-II
Recovery (%)
Presep®
C-PFC
Recovery (%)
PFBS 106 -
PFHxS 116 76
PFOS 107 86
PFDS 107 91
PFBA 120 -
PFPeA 116 -
PFHxA 109 -
PFHpA 110 -
PFOA 110 88
PFNA 109 96
PFDA 110 91
PFUnDA 108 90
PFDoDA 109 89
PFTeDA 97
PFHexDA 105
PFODA 103
※The components indicated by boldface are
  assayed in product test.



Column: Wakopak® Wakosil-II 3C18 RS (2.0 mm x 150 mm)
Eluent :A)10 mmol/L CH3COONH4 in H2O
     B)CH3CN
Gradient: 0-10 min. B = 35 - 90%,10 - 15 min. B = 35%
Flow Rate: 0.2 mL/min. at 40°C
Injection Vol.: 10 ng/mL (CH3OH) (PFBA 50 ng/mL, PFODA 20 ng/mL), 5 μL
Curtain GAS: 30
Collision Gas: 3
lonSpray Voltage: -4,500
Temperature: 400°C
lon Source Gas1 :70
lon Source Gas2 :80
peak No. name Q1/Q3
1 PFBA 212.9/168.9
2 PFPeA 262.8/219.1
3 PFHxA 312.9/268.6
4 PFBS 298.8/79.6
5 PFHpA 362.8/318.7
6 PFOA 412.9/368.9
7 PFHxS 398.8/79.6
8 PFNA 462.7/418.8
9 PFDA 512.9/469.0
10 PFOS 498.8/79.6
11 PFUnDA 562.9/519.0
12 PFDoDA 612.9/568.9
13 PFDS 598.9/79.9
14 PFTeDA 712.9/669.0
15 PFHexDA 812.9/769.0
16 PFODA 912.8/168.9

Description Wako Cat. No. (Pkg. Size)
Presep®-C PFC (Short) 297-49651   (10 units x 5)
Presep® PFC-II (60 mg / 3 mL) 291-33441 (10 units x 10)




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