Solvents for Environment Analysis

The contents of endocrine disruptors, such as phthalate esters, 2-ethylhexyl adipate, alkyl phenols, bisphenol A and styrene dimer/trimer, in the solvents are measured by using high-resolution GC/MS, and it is confirmed that the solvents contain almost no such substances. They are suitable for analytical test of endocrine disruptors.

Product Name Grade Package Size Wako Cat. No.
Acetone for Environment Analysis 500 mL 018-17815
Acetonitrile for Environment Analysis 500 mL 015-17825
Dichloromethane for Environment Analysis 500 mL 041-28055
Diethyl Ether for Environment Analysis 500 mL 048-28065
Ethanol (99.5) for Environment Analysis 500 mL 055-06895
Heptane for Environment Analysis 500 mL 084-07985
Hexane for Environment Analysis 500 mL 085-07655
Methanol for Environment Analysis 500 mL 135-13855
Methyl Acetate for Environment Analysis 500 mL 134-14285
Solvent for Estradiol Analysis

This solvent is suitable for elution for pretreatment by solid-phase extraction in analysis of estradiol.

Product Name Grade Package Size Wako Cat. No.
Dichloromethane for Estradiol Analysis 500 mL 043-28375
Solvent for Aldehydes Analysis

This solvent is designed to determine the quantities of 6 aldehydes (formaldehyde, acetaldehyde, etc.) in liquid chromatography. They are guaranteed to have passed the test for suitability to analysis of aldehydes.

Product Name Grade Package Size Wako Cat. No.
Acetonitrile for Aldehydes Analysis 100 mL 017-17743
200 mL 011-17741
Impurities which may be included in solvents and method of removing contaminants
Impurities  Causes of inclusion Influence  Method of removing
Particulates, such as dust Use of contaminated containers during transferring The particulates clog the line filter, collect in the pump sealing parts and accumulate on the column head. Membrane filter
Water Glassware and solvent manufacturing process Changes in column efficiency and K value Drying with anhydrous sodium sulfate
Alcohol Stabilizer in CHCl3 and impurities in hydrocarbon Ditto In the case of contamination from hydrocarbon, pass through activated silica gel. In the case of contamination from CHCl3, extract with water, and dry with anhydrous sodium sulfate.
Hydrocarbon in water   Instability of base line during gradient elution Pass through ODS column.
Peroxides in ether Degradation Oxidation of binder phase, reaction with sample, and inactivation or deterioration of column Distill, or pass through activated silica gel.
HCl and HBr in halogenated solvent Degradation Deterioration of column (particularly, binder phase), increase in ultraviolet absorption (bromides), and reaction with stainless steel Pass through activated silica gel, etc.
BHT and hydroquinone Antioxidant in THF Increase in ultraviolet absorption Distill.
Dissolved oxygen During manufacture of solvent Deterioration of polystyrene-based packing material, and reaction with sample Deaerate with degasser or ultrasonic waves under reduced pressure.
Unknown ultraviolet absorbing materials   Instability or drift of base line during gradient elution, or, in the case of high detection sensitivity, increase in background Use activated silica gel or alumina. In the case of organic compounds, distill. In the case of inorganic compounds, recrystallize or pass through an ion- exchange column.
High boiling compounds   Contamination of samples collected from preparative HPLC Distill.
Algae in water Growing while solvent is left unused for long time Clogging of line filter and column inlet sintered filter Distill together with alkaline permanganate, or discard.