AFLAKING is an immunoaffinity column developed for cleanup of aflatoxins from food.
With conventional immunoaffinity columns, it was required to dilute organic solvent extracts to 2 % or so, and, as the result of this, turbidity was caused, and analysis of spices, etc. was difficult.
AFLAKING is resistant to the solvents of 20 % acetonitrile and 40 % methanol which are widely used for extraction and ensures easy and quick cleanup of a wide range of food, such as nuts, cereals and spices.
It can clean up aflatoxins B1, B2, G1 and G2 simultaneously. In addition, it can clean up aflatoxin M1. (M1 recovery: 87 %)
It conforms to Notice No. 0728004 of Pharmaceutical and Food Safety Bureau of the Ministry of Health, Labour and Welfare.
- Roasted
peanuts
Corn grits Pearl barley Paprika White sesame
seeds
Red pepper Turmeric Coriander
B1 94 100 97 91 97 97 101 92
B2 95 98 95 89 98 98 92 91
G1 78 105 101 99 88 99 89 91
G2 85 103 98 99 86 99 90 92
Note) Recovery (%) in the case of addition of 16 ng/g of total aflatoxin

Description Code No. Wako Cat. No. (Pkg. Size)
AFLAKING AC01-25 308-34201 ( 25 units)
AC01-50 304-34203 ( 50 units)




OCHRAKING is an immunoaffinity column developed for cleanup of ochratoxins in food. It is highly resistant to organic solvents and ensures quick cleanup with a small amount of sample solution.
It is usable at an acetonitrile concentration of up to 30 % for recovery of ochratoxins A and B and up to 48 % for recovery only of ochratoxin A.
The column can be cleaned with purified water. It is unnecessary to prepare ammonium acetate as with the conventional methods.

・Wheat

To determine the amounts of ochratoxins in wheat,cleanup of ochratoxins A and B can be performed only by dilution to 30 % with PBS after extraction with 60 % acetonitrile. To determine only the amount of ochratoxin A, the column can be used at an acetonitrile concentration of up to 48 %.

・Red wine


Description Code No. Wako Cat. No. (Pkg. Size)
OCHRAKING AC02-25 300-83091 ( 25 units)




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