Wakopak® Navi C18-GT is a packed column with glass lined inside wall. High purity titanium is used for frit at both ends of the column to minimize the nonspecific adsorption.
A Selection Guide for Each Column
Avoids contact with metal (Fe), and inhibits the influence of metal coordination compounds to an absolute minimum Peak shape and recovery ideal for trace analysis in a body sample
A Selection Guide for Each Column
Column: Wakopak® Navi C18-5 GT
Eluent: CH3CN/H2O/H3PO4=60/40/0.1(v/v/v)
Flow Rate: 1.0 mL/min. at 35℃
Detection: UV 254 nm 0.004 AUFS
Injection Volume:5μL

Description Column Size Joint Type
(W: Waters type; D: DuPont type)
Wako Cat. No.( Pkg. Size)
Wakopak® Navi C18-5 2.0 mm × 50 mm W 233-60353 ( 1 unit )
2.0 mm × 150 mm D 234-60361 ( 1 unit )
W 230-60363 ( 1 unit )
3.0mm × 150mm D −   ( 1 unit )
W 231-60393 ( 1 unit )
3.0mm × 250mm D −   ( 1 unit )
W 234-60403 ( 1 unit )
4.6 mm × 150 mm D 231-60511 ( 1 unit )
W 237-60513 ( 1 unit )
4.6 mm × 150 mm D 235-60531 ( 1 unit )
W 231-60533 ( 1 unit )
Wakopak® Navi C18-5 GT 2.0mm × 150mm D −   ( 1 unit )
4.6mm × 150mm D −   ( 1 unit )
4.6mm × 250mm D −   ( 1 unit )

The filler is end-capped after dococyl (C22) modification. By using mobile phase with high water content,
purification and retention characteristics higher than that of C18 can be obtained.
Separation of basic compounds
Separation of basic compounds graph
Column: Wakopak® Navi C22-5(4.6 mm×250 mm)
Eluent: 50mM NaH2PO4(pH 2.4)
Flow Rate: 1.0 mL/min. at 40℃
Detection: UV 235 nm 0.04 AUFS
Injection Volume:5μL
Analysis of Oxine-Cu
Analysis of Oxine-Cu graph
Column: Wakopak® Navi C22-5(4.6 mm×250 mm)
Eluent: 20mM NaH2PO4(pH 2.5)
Flow Rate: 1.0 mL/min. at 35℃
Detection: UV 210 nm 0.04 AUFS
Injection Volume:10μL

Description Column Size Joint Type *
* W: Waters type; D: DuPont type
Wako Cat. No.( Pkg. Size)
Wakopak® Navi C22-5 2.0 mm × 150 mm D −   ( 1 unit )
W 232-60703 ( 1 unit )
3.0mm × 150mm D −   ( 1 unit )
W −   ( 1 unit )
3.0mm × 250mm D −   ( 1 unit )
W 230-60743 ( 1 unit )
4.6 mm × 150 mm D 237-60851 ( 1 unit )
W 233-60853 ( 1 unit )
4.6 mm × 250 mm D 231-60871 ( 1 unit )
W 237-60873 ( 1 unit )

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