Application ⇒ Refer to A-12, A-24, A-29, A-32, A-33
1. Full porous, spherical 3μm packing.
2. Improved the theoritical plates (N >100,000/m)and the separation capability compared with the 5μm.
  packings. This has made column length shortened.
3. HG, RS, AR types are available. Easy to change the method to another analysis condition.
4. Natural resources saving can be achieved by reducing quantity of solvent.
5. Usable in analysis of small quantity of samples and sensitive analysis.
6. Compliant to LC/MS.
A Selection Guide for Each Column
Sample:
1)Methyl p -Hydroxybenzoate
2)Etyl p -Hydroxybenzoate
3)Isopropyl p -Hydroxybenzoate
4)n -Propyl p -Hydroxybenzoate
5)sec-Butyl p -Hydroxybenzoate
6)Isobutyl p -Hydroxybenzoate
7)n -Butyl p -Hydroxybenzoate

Eluent :CH3OH/5mM Citrate buffer(pH4.0)
Flow Rate :0.8ml/min. at 40℃
Detection :UV 270nm
Semimicro Columun
Suitable for LC/MS due to the lower flow rate
(in case of 1.0 ~ 2.0 mm ID).
Achieved the smaller solvent in analysis.
3 mm (I.D.) column
3.0 mm (ID) columns achieves 2 times higher sensitivity and half quantity of solvent with conventional devices.

Separation of basic compounds
Separation of basic compounds graph
Eluent :
A)CH3OH/H2O
=80/20(v/v)
B)CH3CN
0-4min. B   10%
4-7min. B 10 →100%
7-25min. B   100%
Temp :30℃
Detection :
UV 254nm
Sample:
1)Naphthalene
2)Acenaphthylene
3)Acenaphthene
4)Fluorene
5)Phenanthrene
6)Anthracene
7)Fluoranthene
8)Pyrene
9)Benz[a]anthracene
10)Chrysene
11)Benzo[e]pyrene
12)Benzo[b]fluoranthene
13)Benzo[k]fluoranthene
14)Benzo[a]pyrene
15)Dibenz[a,h]anthracene
16)Benzo[g,h,i]peryrene
17)Indeno
  [1,2,3-cd]pyrene
Analysis of Oxine-Cu
Analysis of Oxine-Cu graph
Column :Wakopak® Wakosil-Ⅱ 5C18 HG
Eluent :CH3CN/H2O=60/40(v/v)
Temp :R.T.
Detection:UV 254nm
Sample :1)Uracil 2)Benzene
      3)Naphthalene





Description Column Size Joint Type
(W: Waters type; D: DuPont type)
Wako Cat. No.( Pkg. Size)
Wakopak® Wakosil-Ⅱ 3C18 HG 3.0 mm × 75 mm W 234-50253 ( 1 unit )
3.0 mm × 150 mm W 231-50263 ( 1 unit )
4.6 mm × 75 mm W 230-50353 ( 1 unit )
4.6 mm × 150 mm W 234-50373 ( 1 unit )
Wakopak® Wakosil-Ⅱ 3C18 RS 3.0 mm × 75 mm W − ( 1 unit )
3.0 mm × 150 mm W 236-50453 ( 1 unit )
4.6 mm × 75 mm W 235-50543 ( 1 unit )
4.6 mm × 150 mm W 239-50563 ( 1 unit )
Wakopak® Wakosil-Ⅱ 3C18 AR 3.0 mm × 75 mm W 239-50063 ( 1 unit )
3.0 mm × 150 mm W 236-50073 ( 1 unit )
4.6 mm × 75 mm W 235-50163 ( 1 unit )
4.6 mm × 150 mm W 239-50183 ( 1 unit )


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