Wakopak® Cartridge Columns are cartridge type columns filled with Wakosil®-II 5C18 Series. The cartridges can be replaced easily by screwing manually. They are useful particularly as guard columns which must be replaced in routines and contribute toward reduction of running cost.

1. Easy replacement of columns without special tools
2. Easy-to-use size
3. Low cost and high performance
Description Column Size Wako Cat. No.( Pkg. Size)
Wakopak®Cartridge Column WS-Ⅱ 5C18 HG 4.6 mm × 150 mm 237-01371 ( 1 unit )
Wakopak®Cartridge Guard Column WS-Ⅱ 5C18 HG 4.6 mm × 10 mm 234-01381 ( 3 unit )
Wakopak®Cartridge Guard Column WS-Ⅱ 5C18 RS 4.6 mm × 10 mm 234-01521 ( 3 unit )
Wakopak®Cartridge Guard Column WS-Ⅱ 5C18 AR 4.6 mm × 10 mm 237-01511 ( 3 unit )
Wakopak®Cartridge Holder for 150 mm - 234-01401 ( 1 unit )
Wakopak®Cartridge Holder for 10 mm - 231-01391 ( 1 unit )
* The columns conform to the specifications for DuPont type (D).
* The cartridge holders are provided with stainless steel male nuts and PEEK ferrules.
* The wetted parts of both ends of the cartridge columns are made of PEEK. To prevent damage to the parts, use the supplied PEEK ferrules for connection.
Refer to A-23, A-29
High-purity silica gels differing in micropore diameter are used as raw materials. (See page #34.)
Wakosil-II 5C18-100 has a high carbon content of 22% and shows a high retention capacity.

Column: Wakopak® Wakosil-Ⅱ 5C18(4.6 mm×150 mm)
Eluent: CH3OH/20 mM KH2PO4-H3PO4 buffer
     (pH2.7)=5/95(v/v)
Flow Rate: 1.0 mL/min. at 30℃
Detection: UV 254 nm
Column: Wakopak® Wakosil-Ⅱ 5C18(4.6 mm×150 mm)
Eluent: 7.5 mM NaH2PO4 buffer
Flow Rate: 1.0 mL/min. at 40℃
Detection: UV 270 nm


Column: Wakopak® Wakosil-Ⅱ 5C18-100(4.6 mm×150 mm)
Eluent: A)0.1 M H3PO4
     B)CH3CN/0.1M H3PO4
     40% B → 100% B (20 min)
     100% B (20 min)→
Flow Rate: 1.0 mL/min. at 50℃
Detection: UV 280 nm 0.16 AUFS
Column: Wakopak® Wakosil-Ⅱ 5C18-100(4.6 mm×150 mm)
Eluent: CH3CN/H2O containing 0.05% EDTA・2Na
     =60/40(v/v)
Flow Rate: 1.0 mL/min. at RT
Detection: UV 254 nm 0.32 AUFS



Description Column Size Joint Type
* W: Waters type; D: DuPont type
Wako Cat. No.( Pkg. Size)
Wakopak®Wakosil-Ⅱ 5C18 3.0 mm × 150 mm W 233-59623 ( 1 unit )
4.6 mm × 150 mm D 230-51811 ( 1 unit )
W 236-51813 ( 1 unit )
Wakopak®Wakosil-Ⅱ 5C18-100 3.0 mm × 150 mm W 235-59683 ( 1 unit )
4.6 mm × 150 mm D 235-52081 ( 1 unit )
W 231-52083 ( 1 unit )


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