Column Size : 4.6 mm×150 mm
Eluent : CH3CN/H2O=50/50(v/v)
Flow Rate : 1.0 mL/min. at 30℃
Detection : UV 30 nm
Sample : Bufo Steroid Mixture

Column Size : 4.6 mm×150 mm
Eluent :CH3CN/H2O(pH 2.5←85% H3PO4)=30/70(v/v)
Flow Rate : 1.2 mL/min. at 30℃
Detection : UV 254 nm
Sample : 1)Phthalic Acid 2)Phthalide 3)Benzoic Acid





Column : Wakopak® Wakosil 5C18-200T
4.0 mm×150 mm
Eluent : CH3CN/CH3OH/10 mM
NH4H2PO4 buffer(pH 2.5)
containing 0.5 mM SDS
=35/20/45(v/v)
Flow Rate : 1.0 mL/min. at R.T.
Detection : UV 280 nm

Description Column Size Joint Type * Wako Cat. No.( Pkg. Size)
Wakopak® Wakosil 5C18 4.6 mm×150 mm W 239-53983 ( 1 unit )
4.6 mm×250 mm W 233-54003 ( 1 unit )
Wakopak® Wakosil 5C18N 4.6 mm×150 mm W 233-54483 ( 1 unit )
4.6 mm×250 mm W 233-54503 ( 1 unit )
Wakopak® Wakosil 5C18T 4.6 mm×150 mm W 236-54233 ( 1 unit )
4.6 mm×250 mm W 230-54253 ( 1 unit )
Wakopak® Wakosil 5C18-200 4.6 mm×150 mm W
4.6 mm×250 mm W 231-55523 ( 1 unit )
Wakopak® Wakosil 5C18-200N 4.6 mm×150 mm W 231-56003 ( 1 unit )
4.6 mm×250 mm W 235-56023 ( 1 unit )
Wakopak® Wakosil 5C18-200T 4.6 mm×150 mm W 234-55753 ( 1 unit )
4.6 mm×250 mm W 238-55773 ( 1 unit )
Wakopak® Wakosil 5C18 AR 4.6 mm×150 mm W 236-54733 ( 1 unit )
4.6 mm×250 mm W 230-54753 ( 1 unit )
Wakopak® Wakosil 7C18 4.6 mm×150 mm W 233-54983 ( 1 unit )
4.6 mm×250 mm W 237-55003 ( 1 unit )
Wakopak® Wakosil 10C18 4.6 mm×150 mm W 230-55233 ( 1 unit )
4.6 mm×250 mm W 234-55253 ( 1 unit )
Wakopak® Wakosil 10C18-200 4.6 mm×150 mm W 238-56253 ( 1 unit )
4.6 mm×250 mm W 232-56273 ( 1 unit )
*All Joint Types: Waters


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