Application: Refer to A-13
Like Wakosil®-II C18 Series, this highly purified spherical C8 silica gel series uses high-purity silica gel with a low metallic content as a raw material and can clearly analyze basic substances and coordination compounds. Since the material has shorter alkyl chains compared to ODS (C18) and the retention of highly hydrophobic substances in the material is low, components differing in polarity can be analyzed in a short time. As in C18 Series, HG type and RS type are available.
A Selection Guide for Each Column
@ Eluent : CH3CN/H2O=60/40(v/v)
Flow Rate : 1.0ml/min. at 35℃
Detection : UV 254nm

Sample :
1)Uracil
2)Benzene
3)Naphthalene

N=Plates for Last Peak,
K'=Capacity factor,
S=Assymetry factor
(10% Height of Last Peak)

Separation of basic compounds
Separation of basic compounds graph
Eluent : CH3CN/H2O=60/40(v/v)
Flow Rate : 1.0ml/min. at 35℃
Detection : UV 254nm 0.16AUFS
Analysis of Oxine-Cu
Analysis of Oxine-Cu graph
Eluent : CH3OH/20mM KH2PO4,K2HPO4(pH6.5)
=62/38(v/v)
Flow Rate : 1.0ml/min. at 40℃
Detection : UV 254nm

Description Particle Size Column Size Joint Type
(W: Waters type; D: DuPont type)
Wako Cat. No.( Pkg. Size)
Wakopak® Wakosil-Ⅱ 5C8 HG 5 μm 2.0 mm × 150 mm W 233-52423 ( 1 unit )
4.6 mm × 150 mm W 230-52553 ( 1 unit )
4.6 mm × 250 mm W 234-52573 ( 1 unit )
Wakopak® Wakosil-Ⅱ 5C8 RS 5 μm 2.0 mm × 150 mm W 234-52713 ( 1 unit )
3.0 mm × 150 mm W 238-52733 ( 1 unit )
4.6 mm × 150 mm W 231-52843 ( 1 unit )
4.6 mm × 250 mm W 235-52863 ( 1 unit )
Wakopak® Wakosil-Ⅱ 3C8 RS 3 μm 2.0 mm × 150 mm W 235-52243 ( 1 unit )
3.0 mm × 150 mm W 239-52263 ( 1 unit )
4.6 mm × 150 mm W 235-52363 ( 1 unit )


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