Wakopak® Fluofix® is a reversed-phase HPLC column packed with highly purified spherical 5μm silica-gel (SiO2 > 99.99%), to which fiuorocarbon chains are bonded.
Fluofix®-II 120E can provide the best separation for fluorine compounds, isomers in particular.
The end-capping efficiency of Fluofix®-II 120E is remarkably improved and its non-specific adsorption is minimized compared with Fluofix 120E. Because of its high retention capacity,Fluofix®-II 120E has higher selectivity and versatility.
The separation mode of this column is basically reversed phase like hydrocarbon modified silicagel column such ODS, C8, etc., its retention capacity is comparable as C4. Because of its strong hydrophobic/lipophobic as well as its characteristic fluorinated phases, this column specifically shows highly retaining and separating capabilities depending on the characteristics the specimens.

1. Improvement in separation capability.
2. High recognition capability of halogen compounds such as fluorides.
3. Basic compounds are also applicable.
4. Recognition of specific compounds by adamant branched fluorocarbon chain.
5. High durability by chemically stable fluorocarbon.
Schematic Surface Model of
Wakopak® Fluofix
Separation of basic compounds
Separation of basic compounds graph 〈Sample〉
1.Uracil
2.Methyl Benzoate
3.Toluene
4.Triphenylene
5.o-Terphenyl
6.Hexafluorobenzene in CH3OH

Column size:4.6mm×250mm
Eluent :
CH3OH/H2O=60/40
Flow Rate :
1.0ml/min at 40℃
Detection :UV 254nm
Injection :5μl
Analysis of Oxine-Cu
Separation of basic compounds graph 〈Sample〉
1.p-Fluoroaniline
2.o-Fluoroaniline
3.m-Fluoroaniline in 30% MeOH

Column size:4.6mm×250mm
Eluent :CH3OH/20mmol/L TBABr=20/80
Flow Rate :
1.0ml/min at 40℃
Detection :UV 254nm
Injection :10μl
TBABr :
Tetrabutylammonium bromide




Separation of basic compounds
Separation of basic compounds graph 〈Sample〉
1. Uracil
2. Benzene
3. Fluorobenzene
4. p-Difluorobenzene
5. o-Difluorobenzene
6. m-Difluorobenzene
7. 1,3,5-Trifluorobenzene
8. 1,2,3,4-Tetrafluorobenzene
9. Pentafluorobenzene
10. Hexafluorobenzene in CH3OH

Column size :
4.6mm×250mm
Eluent :
CH3OH/H2O=60/40
Flow Rate :
1.0ml/min at 40℃
Detection :UV 254nm
Injection :10μl
Analysis of Oxine-Cu
Separation of basic compounds graph Column:
Wakopak® Fluofix 120N
(4.6mm×150mm)

Eluent:
(A)CH3CN
(B)0.1% Phosphoric acid
(A)25 to 80v/v% for 50min.

Flow Rate:1mL / min.

Temperature:40℃

Detection:
UV(230
nm)




Description Purpose Column Size Joint Type
(W: Waters type; D: DuPont type)
Wako Cat. No.( Pkg. Size)
Wakopak® Fluofix-Ⅱ 120E for ordinary analysis 4.6 mm × 150 mm W − ( 1 unit )
4.6 mm × 250 mm W − ( 1 unit )
Wakopak® Fluofix 120E for ordinary analysis 4.6 mm × 150 mm W 231-61993 ( 1 unit )
4.6 mm × 250 mm W 238-62003 ( 1 unit )
Wakopak® Fluofix 120N for acidic compounds 4.6 mm × 150 mm W 235-61893 ( 1 unit )
4.6 mm × 250 mm W 238-61903 ( 1 unit )


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