Silica gel having a particle size of 5 μm and a pore size of 12 nm is chemically bonded to aminopropyl groups. This filler can be used in both normal and reverse phase modes by changing the mobile phase. It is helpful particularly in analysis of monosaccharides and disaccharides and used also for analysis of nucleotide.


Column : Wakopak® Wakosil 5NH2 (4.6 mm × 150 mm)
Eluent : CH3CN/H2O=75/25 (v/v)
Flow Rate : 1.0 mL/min. at 35℃
Detection : Rl × 32, FS 10 mV, ATTEN=1
Injection Volume : 10 μL

Column : Wakopak® Wakosil 5NH2 (4.6 mm × 150 mm)
Eluent : CH3CN/H2O=70/30 (v/v)
Flow Rate : 2.0 mL/min.
Detection : Rl


Column : Wakopak® Wakosil 5NH2 (4.6 mm × 250 mm)
Eluent : CH3CN/0.01M KH2PO4=80/20 (v/v)
Flow Rate : 1.2 mL/min.
Detection : UV 254 nm

Column : Wakopak® Wakosil 5NH2 (4.6 mm × 250 mm)
Eluent : Ethanol/0.1M KH22PO4=10/90 (v/v)
Flow Rate : 1.0 mL/min.
Detection : UV 254 nm

Description Column Size Joint Type *
* All Joint Types:Waters
Wako Cat. No. (Pkg. Size)
Wakopak® Wakosil 5NH2 3.0 mm × 150 mm W 234-63323 ( 1 unit )
3.0 mm × 250 mm W 231-63333 ( 1 unit )
4.0 mm × 150 mm W 231-57603 ( 1 unit )
4.0 mm × 250 mm W 235-57623 ( 1 unit )
4.6 mm × 10 mm W 239-57643 ( 1 unit )
4.6 mm × 30 mm W 236-57653 ( 1 unit )
4.6 mm × 100 mm W 233-57663 ( 1 unit )
4.6 mm × 150 mm W 230-57673 ( 1 unit )
4.6 mm × 250 mm W 234-57693 ( 1 unit )
6.0 mm × 150 mm W 234-57713 ( 1 unit )
6.0 mm × 250 mm W 231-57723 ( 1 unit )
7.5 mm × 300 mm W 236-57751 ( 1 unit )
10 mm × 250 mm W 236-57773 ( 1 unit )
10 mm × 300 mm W 233-57783 ( 1 unit )




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