Refer to p 31
Wakosil®-II 5SIL-AQ is a high-purity silica gel filler for HPLC. The filler has an excellent ability to analyze high-polarity substances, particularly basic substances, which cannot be analyzed satisfactorily by general ODS fillers because the fillers retain small quantities of such substances. It can widen the range of application of HPLC analysis.

1. High-purity silica gel is used. … The influence of metallic impurities can be reduced.
2. Application to LC/MS analysis is easy. … The organic solvent concentration is high, and analysis conditions without the use of ion-pair reagents can be set.

Column :Wakopak® Wakosil-Ⅱ 5SIL-AQ (4.6 mm × 250 mm)
Eluent :CH3CN/0.1 mol/L HCOONH4, pH 3.0 = 85/15
Flow Rate :1.0 mL/min. at 40℃
Detection :UV 260 nm
Injection :each 0.1 mg/mL (H2O), 10 μL

Column :Wakopak® Wakosil-Ⅱ 5SIL-AQ (4.6 mm × 250 mm)
Eluent :CH3CN/0.1 mol/L HCOONH4, pH 3.0 = 85/15
Flow Rate :1.0 mL/min. at 40℃
Detection :UV 254 nm
Injection :each 0.2 mg/mL (EtOH), 10 μL

Column :Wakopak® Wakosil-Ⅱ 5SIL-AQ (4.6 mm × 250 mm)
Eluent :CH3CN/H2O/H3PO4 = 97/2/1 (v/v/v)
Flow Rate :1.0 mL/min. at 40℃
Detection :UV 210 nm
Injection :each 1.0 mg/mL (90% CH3CN), 5 μL




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