Wakosil® 5Diol are fillers for gel filtration obtained by chemical bonding of diol groups (alcoholic hydroxyl groups) to the surfaces of 5-μm totally porous spherical silica gel. Since the fillers use a silica gel base material having higher physical strength compared to polymer base materials, they can ensure stable separation even if they are used continuously at high pressure and high flow rate.
Four types of fillers differing in pore size (6 nm, 12 nm, 20 nm and 30 nm) are available. They are suitable for analysis of water-soluble polymers having a molecular weight of several hundreds to several hundreds of thousands.
Column Size : 8.0 mm × 300 mm
Eluent : A) 0.1 M Na phosphate buffer (pH 7.4)/
        CH3CN=80/20 〔for Diol-60〕
    : B) 0.2 M NaCl in 0.1 M Na phosphate
        buffer (pH 7.0) 〔for Diol-120, 200, 300〕
Flow Rate : 1.0 mL/min. at 40℃
Detection : UV 220 nm
Sample : 1)Thyroglobulin (MW 660,000)
      2)Ferriti n(MW 440,000)
      3)γ-Globulin (MW 160,000)
      4)Bovine serum albumin (MW 67,000)
      5)Ovalbumin (MW 44,000)
      6)β-Lactoglobulin (MW 35,000)
      7)α-Chymotrypsinogen A (MW 22,400)
      8)Myoglobin (MW 18,000)
      9)Ribonuclease A (MW 13,700)
      10)Cytochrome C (MW 12,400)
      11)α-Endorphin (MW 1,746)
      12)Angiotensin-Ⅰ (MW 1,296)
      13)Angiotensin-Ⅱ (MW 1,046)
      14)Angiotensin-Ⅲ (MW 931)
      15)Met-Enkephalin (MW 574)
      16)Valine (MW 117)

For analysis of proteins having a molecular weight of ten thousand to several hundreds of thousands, Diol-120, 200 and 300 are suitable.


Column Size : 8.0 mm × 300 mm
Eluent : 0.2 M NaCl in 0.1 M Na phosphate buffer
     (pH 7.0) 〔for Diol-120, 200, 300〕
Flow Rate : 0.7 mL/min. at 40℃
Detection : UV 280 nm
Sample : 1)Glutamate Dehydrogenase (MW 290,000)
      2)Lactate Dehydrogenase (MW 142,000)
      3)Enolase (MW 67,000)
      4)Adenylate Kinase (MW 32,000)
      5)Cytochrome C (MW 12,400)

Column Size : 8.0 mm × 300 mm
Eluent : 0.2 M NaCl in 0.1 M Na phosphate buffer
      (pH 7.0)
Flow Rate : 1.0 mL/min. at 40℃
Detection : UV 280 nm
Sample : 1)Thyroglobulin (MW 660,000)
      2)γ-Globuline (MW 160,000)
      3)Ovalbumin (MW 44,000)
      4)Myoglobin (MW 18,000)
      5)Vitamin B12 (MW 1,355)

Description Column Size Joint Type *
* All Joint Types: Waters
Wako Cat. No. (Pkg. Size)
Wakopak® Wakosil 5Diol-60 8.0 mm × 300 mm W 238-59553 ( 1 unit )
Wakopak® Wakosil 5Diol-120 W 235-59563 ( 1 unit )
Wakopak® Wakosil 5Diol-200 W 232-59573 ( 1 unit )
Wakopak® Wakosil 5Diol-300 W 239-59583 ( 1 unit )




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