Separation of basic compounds
Wakopak® Wakobeads (WB)-G Series are HPLC columns designed basically for aqueous GPC separation and filled with polyacrylic ester-based porous spherical gel. Although the columns are designed for aqueous GPC, they are uniformly and densely filled with chemically stable gel which hardly swells or shrinks and can be used by mixing the aqueous mobile phase with organic solvents or replacing it with 100% organic solvents. Applicable organic solvents include methanol, acetone,chloroform, dioxane, tetrahydrofuran, N,N-dimethyl formamide and dimethyl sulfoxide. Four types are supplied for exclusion limit molecular weight levels. For protection of the analysis columns, a cartridge pre-column WB-F (holder and 4.0 mm × 10 mm cartridge, 1 pc.) and a replacement cartridge column WB-FC (4 pcs.) are available for each type.


Column Size : 7.8 mm × 300 mm
Eluent : THF
Flow Rate : 1.0 mL/min.
Detection : UV 250 nm
Sample : Polystyrene standards

Column Size : 7.8 mm × 300 mm
Eluent : H2O
Flow Rate : 1.0 mL/min.
Detection : RI
Sample : ●Pullulan ○Polyethylene

Column (filler) type WB-G-20 WB-G-30 WB-G-40 WB-G-50
Column size 7.8 × 300 mm *1
Exclusion limit molecular weight*2 6 × 103 5 × 104 4 × 105 2 × 106
Theoretical plate number 8,000 7,000 8,000 8,000
Working column pressure Below 30 kg/cm2
Maximum working flow rate 1.5 mL/min.
Optimum working flow rate 1.0 mL/min.
*1 Waters type columns are used.
*2 The measurements were obtained by using a 100% aqueous mobile phase.

Column (filler) type WB-G-20 WB-G-30 WB-G-40 WB-G-50
Water-pullulan, PEG 6 × 103 5 × 104 4 × 105 2 × 106
THF-polystyrene 2.5 × 103 5.5 × 104 5.5 × 105 2 × 106
CHCl3-polystyrene 1 × 103 5.5 × 104 4 × 105 2 × 106
DMF-polystyrene 3.5 × 103 6 × 104 4 × 105 2 × 106




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