78P


The column chromatography is generally used for medium- to large-scale fractionation and purification.
We are offering a wide range of carriers made of various materials so that the column chromatography can be applied not only to organic compounds, but also to natural substances and biopolymers.


Column chromatography carrier selection guide
Material Major Separation
Mode
Features Uses
Silica gel Adsorption ・The most common carrier
・It has a high physical strength and
 can be used in a wide range.
・Separation mainly of neutral and
 acid substances
Chemically bonded
silica gel
Reversed-phase
partition
・Easy control of water content
・A wide range of solvents, from
 organic solvents to aqueous solutions,
 can be used as eluents.
・It has a high physical strength and
 can be used in a wide range.
・For a wide range of samples
Alumina Adsorption ・Strong activity, and high separation ability
・It is not reactive with general solvents
 and is less reactive with solutes.
・The activity can be changed in stages
 by changing the water content.
・The ability to adsorb solutes can be
 regulated.
・Acid alumina: Separation of acid
 substances
 (acidic peptide, etc.)
・Neutral alumina:Catecholamine
・Basic alumina: Generally used for
 a wide range of substances,
 such as alkaloid,steroid and terpene
Activated carbon Adsorption ・Excellent adsorption capacity ・Applicable to a wide range of uses
・Adsorption of low- and high-molecular
 dissolved organic matter contained in
 tap water
Florisil
(magnesium silicate)
Adsorption ・High selectivity ・Separation of nitrogen compounds in
 steroids,sex hormones, lipid, alkaloid
 and hydrocarbon,and separation of
 aromatic compounds from residual
 pesticide, antibiotics, pharmaceuticals
 and mixtures of aliphatic and aromatic
 compounds
Polyamide Adsorption ・Larger adsorption capacity compared
 to inorganic adsorbents
・The carrier itself is neutral.
・Dinitrophenyl (DNP) amino acid,
 glycosides,aromatic nitro compounds,
 phenols, bromophenols, phenolic
 alkaloids, etc.
Chemically bonded
cellulose
Ion exchange ・Separation by electrostatic interaction
 between solute and carrier leaving group
  (ion exchange group)
・Functional proteins, such as enzymes,
 and biopolymers


Wako Product Information - Chromatography - 8th Edition

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