Presep® RPP-SAX Type 3S is a sample pretreatment column filled with a polymer resin obtained by introducing anionexchange groups to a polymer having both hydrophilic and hydrophobic groups, and it has both reversed-phase and anionexchange properties. The column is usable for selective extraction of acidic compounds and extraction of pharmaceutical products and their metabolites from biological samples (urine, blood, etc.)




*1 8 mL of 2 mol/L CH3COONa (pH 7.0) buffer containing reference standard is used.
*2 Measurement by GC/MS or HPLC can be performed by changing the re-dissolved solution according to the compound to be measured.

Column :BPX-5(0.32 mmφ × 30 m, 0.25 μm film thickness)
Injection method :High-pressure split-less
Vaporization chamber
temperature :250℃
Column temperature :70℃(2 min)→ 180℃/min → 320℃ (7 min)
High-pressure injection:100 kPa (1 min)
Injection volume :3 μL
Ionization mode :El
Interface temperature:280℃
Ion source temperature:230℃
Measurement mode :SIM mode
Ionization voltage :70 eV


Compound Standard additive
amount
Presep® RPP-SAX
ng/Column Recovery (%)
Methamphetamine 100 117
Metoxyphenamine 100 108
Nicotine 200 94
Theophylline 500 122




*1 8 mL of 2 mol/L CH3COONa (pH 7.0) buffer containing reference standard is used.
*2 Measurement by GC/MS or HPLC can be performed by changing the re-dissolved solution after drying according to the compound to be measured.

Column :Wakopak® Wakosil-Ⅱ 5C18 HG ( 4.6 mm× 50 mm)
Eluent :A)0.1% H3PO4 B)CH3CN
Time Program:0-15 min. B 5-85%, 15-25 min. B 5%
Flow Rate :1.0 mL/min. at 40℃
Injection Vol. :5 μL
Detection :UV 210 nm


Compound Standard additive
amount
Presep® RPP-SAX
ng/Column Recovery (%)
neutral
Theophylline 5 97.1
Scopolamine
Hydrobromide
25 106.6
Caffeine 5 101.7
basic
Atropine Sulfate 25 108.0
Barbital 10 102.7
Nortriptyline
Hydrochloride
10 103.5
acid
Salicylic Acid 10 106.8
Naproxen 10 83.9

Description Amount of filler
(mg/cartridge)
Wako Cat. No. (Pkg. Size)
Presep® RPP-SAX Type3S 60 / 3 mL 297-33301 ( 10 units × 10 )




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