Carrers for TLC

Presep®-C Ozone Scrubber (Wako Cat No. 293-40351)

Presep®-C DNPH (Wako Cat No. 290-34251)

Presep®-C DNPH(Short) (Wako Cat No. 291-43951)

Analysis systems for aldehydes to be controlled under Offensive Odor Control Law and Air Pollution Control Law and aldehydes causing sick house syndrome (indoor air pollution)

Features
    Presep®-C DNPH Series
  • Low blank values
    (Contamination by formaldehyde, acetaldehyde and acetone is prevented as much as possible.)
  • Easy-to-use individual packaging
  • Presep®-C DNPH (Short)
  • Low air flow resistance and sampling at high flow velocity
  • Suitable for collection of indoor air (in conformity with the law of the Ministry of Health, Labour and Welfare (Japan))
Solid-phase extraction conditions HPLC analysis conditions
■Specifications
  Presep®-C DNPH(Short) Presep®-C DNPH
Substrate

Granular silica gel

Particle size: 150 to 425 μm Pore size: 7.0 nm
Specific surface area: 450 cm2/g

Granular silica gel
Particle size: 75 to 150 μm
Pore size: 7.0 nm
Specific surface area: 450 cm2/g

Amount of packing material

Approx. 0.4 g (silica gel)/ cartridge Approx. 0.8 g (silica gel)/ cartridge

Amount of DNPH

Approx. 0.9 mg/cartridge Approx. 1.8 mg/cartridge
Column size

Overall length: 3.8 cm

Max. width: 1.9 cmφ
Packed portion: 1.0φ x 0.8 cm

Overall length: 5.0 cm

Max. width: 1.9 cmφ
Packed portion: 1.0φ x 1.6 cm

Sample load Approx. 75 μm (as formaldehyde) Approx. 150 μm (as formaldehyde)
■Cartridge blank

After acetonitrile was fed into Presep®-C DNPH cartridge and the amount of eluate was adjusted to 5 mL with acetonitrile, 10 μL were injected. For comparison, the chromatograph obtained with the product of company A treated in the same manner is shown.

Column
Eluent
:Wakopak® Wakosil DNPH-II (4.6 mm × 150 mm)
:A)Wakosil® DNPH-II Eluent A
B)Wakosil® DNPH-II Eluent A
0-10 min. B conc. 0-100%
10-16 min. B conc. 100%
Flow Rate
Detection
Injection Volume
:1.0 mL/min. at 35°C
:UV 360 nm
:10 μL