Refer to p.46
Column : Wakopak® Wakosil-Ⅱ 5C18 RS Prep
EIuent : A)CH3COOH/H2O = 1/99 (v/v)
     B)CH3CN/H2O = 30/70 (v/v)
     0-19 min. B 0%, 19-37 min. B 100%, 37-41 min. B 0%
Detection : UV 280 nm 3.2 AUFS
Sample : Crude Arbutin (100 mg/mL in H2O)
      1)Arbutin

Column Size : 4.6 mm × 250 mm
Flow Rate : 0.8 mL/min. at R.T.
Pressure : 45 kg/cm2
Sample Size : 20 μL
20 mm × 250 mm
15.1 mL/min. at R.T.
46 kg/cm2
380 μL
33 mm × 250 mm
41.2 mL/min. at R.T.
49 kg/cm2
1.0 mL
50 mm × 250 mm
92.6 mL/min. at R.T.
44 kg/cm2
2.3 mL

Refer to p.56
Column : Wakopak® Wakosil-Ⅱ 5SIL Prep
EIuent : n-Hexane/Ethyl Acetate = 100/0.5 (v/v)
Detection : UV 254 nm 0.8 AUFS
Sample : 1)Hexyl Benzoate 10 mg/mL
      2)Propyl Benzoate 10 mg/mL
      3)Methyl Benzoate 10 mg/mL (in n-Hexane)

Column Size : 4.6 mm × 250 mm
Flow Rate : 1 mL/min. at R.T.
Pressure : 18 kg/cm2
Sample Size : 10 μL
20 mm × 250 mm
18.9 mL/min. at R.T.
18 kg/cm2
190 μL
33 mm × 250 mm
51.5 mL/min. at R.T.
16 kg/cm2
500 μL
50 mm × 250 mm
115.8 mL/min. at R.T.
14 kg/cm2
1.15 mL
The same separation results can be obtained by setting the flow rate and sample quantity in proportion to the column sectional area.




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