〈Eluent : CH3OH/H2O=80/20 (v/v)〉

Column : Wakopak® Wakosil-Ⅱ 5C18 HG Prep
EIuent : CH3OH/H2O=80/20 (v/v)
Detection : UV 220 nm 0.16 AUFS
Sample : Naphthalene 0.2 mg/mL
Column Size (Injection Volume):
   ○4.6 mm × 250 mm (5 μL)
   ●20.0 mm × 250 mm (95 μL)
   □33.0 mm × 250 mm (258 μL)
   ■49.5 mm × 250 mm (579 μL)

〈Eluent : CH3CN/H2O=60/40 (v/v)〉

Column : Wakopak® Wakosil-Ⅱ 5C18 HG Prep
EIuent : CH3CN/H2O=60/40 (v/v)
Detection : UV 254 nm 0.16 AUFS
Sample : Naphthalene 0.2 mg/mL
Column Size(Injection Volume) :
   ○4.6 mm × 250 mm (5 μL)
   ●20.0 mm × 250 mm (95 μL)
   □33.0 mm × 250 mm (258 μL)
   ■49.5 mm × 250 mm (579 μL)

Column : Wakopak® Wakosil-Ⅱ 5C18 HG Prep
Eluent : CH3CN/H2O
Sample : Propyl Benzoate
Column Size Flow Rate injection
○4.6 mm × 250 mm 1.0 mL/min. 0.018 mg
●20.0 mm × 250 mm 18.9 mL/min 0.034 mg
□33.0 mm × 250 mm 51.5 mL/min. 0.926 mg
■49.5 mm × 250 mm 115.8 mL/min. 2.084 mg
The same quantities of sample (propyl benzoate) are injected to the columns of the same size.




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