Refer to p.50

EIuent :CH3CN/20 mM KH2PO4
      =10/90 (v/v)
Flow Rate : 1.0 mL/min. at 40℃
Detection : UV 210 nm 0.16 AUFS
Inj. Volume : 3 μL
Sample :
1)Acesulfame K (50 μg/mL)
2)Saccharin Sodium (20 μg/mL)
3)Aspartame (100 μg/mL)
EIuent : CH3CN/0.1% H3PO4,
      50 mM SHS=8/92 (v/v)
Flow Rate : 1.0 mL/min. at 40℃
Detection : UV 210 nm 0.128 AUFS
Inj. Volume : 3 μL



SHS:Hexanesulfonic Acid Sodium Salt
Sample :
1)L-Ascorbic acid (V.C.)
2)Nicotinic acid
3)Nicotinamide
4)Phridoxine・HCl (V.B6)
5)Thiamine・HCl (V.B1)
6)Caffeine
7)D-Biotin (V.H)
8)Riboflavin (V.B2)
The use of the C8 columns enables reduction of analysis time without degrading the separation of peaks.
They are useful particularly for analysis of highly hydrophobic compounds.


CoIumn Size : 4.6 mm × 150 mm
EIuent : CH3CN/H2O=50/50 (v/v)
Flow Rate : 1.0 mL/min. at 30℃
Detection : UV 254 nm 0.256 AUFS
Sample : 1)Pyridine
      2)Phenol
      3)Methyl Benzoate
      4)3-Methylindole
As the result of comparison of the pyridine peak shapes and the retention times, Wakosil®-II 5C8 RS is regarded as a C8 column having a high end capping efficiency.


CoIumn Size : 4.6 mm × 150 mm
EIuent : CH3OH/20 mM KH2PO4, K2HPO4 (pH6.5)
=62/38 (v/v)
Flow Rate : 1.0 mL/min. at 40℃
Detection : UV 254 nm 0.128 AUFS
Sample : 1)Protriptyline Hydrochloride
      2)Nortriptyline Hydrochloride
      3)Doxepin Hydrochloride
      4)Imipramine Hydrochloride
      5)Amitriptyline Hydrochloride
      6)Trimipramine Maleate




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