Refer to page #92, #93, #94, #95

Bisphenol A and nonylphenol were added to tap water at a concentration of 4.0 μg/L each, and the obtained solution was concentrated 250 times with Presep®-C Agri (Short). Then, it was subjected to HPLC analysis with Wakopak® Wakosil Agri-9.

Conditions of extraction of solid phase of phenols
Solid phase extraction column
Presep®-C Agri (Short)
Extraction conditions
Preparation of test water: Adjustment to
pH3.5 with 1M HCl
Conditioning of solid phase extraction column
Conditioning of solid phase extraction column:
   ↓ ①CH3CN 3 mL 
     ②H2O 3 mL
Test water feed:250 mL
Cleaning:5% CH3OH 3 mL
Elution:CH3CN 6 mL
Concentration: Under N2 flow (approx. 0.2 to 0.3 mL)
Re-dissolution: Dilution to 1 mL with CH3CN
 HPLC 10μL

Refer to page #41

Column : Wakopak® Wakosil-Ⅱ 5C8 HG(4.6 mm×250 mm)
Eluent : CH3CN/H2O=60/40(v/v)
Flow Rate : 1.0 mL/min. at 40℃
Detection : UV 225 nm, 0.04 AUFS.
Injection : 10 μL, 10 μg/mL(CH3CN)

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