Phthalic esters were analyzed with three kinds of columns. For simultaneous analysis of components having significant differences in polarity under isocratic conditions, Wakopak® Wakosil Agri-9 is suitable.

Refer to page #42

Column : Wakopak® Wakosil Agri-9(4.6 mm×150 mm)
Eluent : CH3CN/H2O=50/50
Flow Rate : 1.0 mL/min. at 35℃
Detection : UV 254 nm

Column : Wakopak® Wakosil-Ⅱ 5C18 AR(4.6 mm×150 mm)
Eluent : CH3CN/H2O=80/20
Flow Rate : 1.0 mL/min. at 35℃
Detection : UV 254 nm
Refer to pp.41

Column : Wakopak® Wakosil-Ⅱ 5C18 HG(4.6 mm×150 mm)
Eluent : CH3CN/H2O=80/20
Flow Rate : 1.0 mL/min. at 35℃
Detection : UV 254 nm



Column : Wakopak® Wakosil-Ⅱ 5C18 HG(4.6 mm×150 mm)
Eluent : A)CH3CN/H2O=50/50, B)CH3CN
0.5 min. B conc. 20%, 5-25 min. B conc. 20-100%,
25-30 min. B conc. 100%
Flow Rate : 1.0 mL/min. at 35℃
Detection : UV 254 nm


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