Example of reduction of analysis time (veterinary drug mixture PL-2-1
Column: Wakopak® Wakosil-Ⅱ 3C18 HG




Column : Wakopak® Wakosil-Ⅱ 3C18 HG
Eluent : A)0.1 % HCOOH in H2O
     B)0.1 % HCOOH in CH3CN**
Time program :
Flow Rate : 0.2 mL/min. at 40°C
Injection Vol. :
    0.1 ppm、4 μL (3.0 × 150 mm)、3μL (2.0 × 100 mm)


ESI, MRM
Ion Spray Voltage : 5500 V (pos)、−4500 V (neg)
Temperature : 400°C (pos), 500°C (neg)
Curtein Gas : 20
Collison Gas : 6
Ion Source Gas 1 : 50
Ion Source Gas 2 : 80
System : 3200 Q TRAP (ABI)

*:
 Formic acid for LC/MS (approx. 99 %) 
   (Wako Cat. No. 067-04531)
Ultrapure water for LC/MS (Wako Cat. No. 214-01301)

**:
 0.1 vol% formic acid – acetonitrile for LC/MS (Wako Cat. No. 062-04721)
Veterinary drug mixture PL-2-1: 24-component mixture
Peak
No.
Analyte Peak Name Analyte Mass Ranges
(amu)
Analyte
Retention
Time (min)
Analyte
Retention
Time (min)
Precursor Product Mode 3×150mm 2×100mm
1 5-Propylsulfonyl-1H-
benzimidazole-2-amine
240.1 133.2 11.2 4.7
2 Levamisole 207.1 91.2 11.3 4.7
3 Thiabendazole 202.1 175.1 11.8 4.9
4 Trimethoprim 291.1 230.3 12.0 4.9
5 Sulfadiazine 251.1 92.2 12.3 5.0
6 Ormetoprim 275.2 123.1 12.5 5.1
7 Sulfathiazole 256.0 156.0 12.8 5.2
8 Sulfapyridine 250.0 156.2 13.2 5.3
9 Sulfamerazine 265.1 92.2 13.8 5.5
10 Thiamphenicol 355.9 308.0 14.4 5.7
11 Sulfadimidine 279.1 92.1 15.0 5.9
12 Sulfamethoxypyridiazine 281.1 156.1 15.0 5.9
13 Sulfamonomethoxine 281.1 156.1 16.1 6.2
14 Sulfachlorpyridazine 286.1 157.1 16.7 6.4
15 Sulfamethoxazole 254.0 156.0 17.5 6.6
16 Sulfadoxine 311.1 156.1 17.4 6.6
17 Ethopabate 238.2 136.0 19.3 7.2
18 Sulfaquinoxaline 301.0 156.2 19.4 7.2
19 Sulfadimethoxine 311.1 156.2 19.4 7.2
20 Sulfanitran 336.1 134.1 21.9 7.9
21 β-Trenbolone 271.2 165.3 23.2 8.3
22 α-Trenbolone 271.2 165.2 23.7 8.5
23 Melengestrol Acetate 397.2 279.4 31.3 10.7
1 Zeranol 321.1 277.2 24.2 8.7
Veterinary drug mixture standard solution PL-2 (Wako Cat. No. 227-01691)




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