Simultaneous analysis using Wakopak® Wakosil-II 5C18 RS

Column : Wakopak® Wakosil-Ⅱ 5C18 RS (4.6 mm × 150 mm)
Eluent : CH3OH/0.1 % H3PO4, 5 mM SHS=10/90 (v/v)
Flow Rate : 1.0 mL/min. at 40℃
Detection : UV 210 nm, 0.16 AUFS.
)1-Hexanesulfonoic Acid Sodium salt

Sample:
(1) L(+)-Ascorbic acid (V.C); (2) Nicotinic Acid;
(3) Nicotinamide; (4) Pyridoxine·HCl (V.B6); (5) Caffein;
(6) Thiamine (V.B1); (7) d-Biotin (V.H); (8) Riboflavin (V.B2)

Wakopak® Wakosil-II 5C18 RS has high separation and retention capacities even for aqueous eluents. (See pages 41 and A-8.)

Column : Wakopak® Navi C30-5 (4.6 mm × 250 mm)
Eluent : CH
3OH/CH3CN=25/75 (v/v)
Flow Rate : 1.0 mL/min. at 40℃
Detection : UV 280 nm, 0.16 AUFS
Injection Volume : 10 μL
Sample:
(1) Vitamin K3; (2) Vitamin A; (3) Vitamin D2; (4) Vitamin D3; (5) Vitamin E; (6) Vitamin E Acetate; (7) Vitamin K1
This column can separate γ-β-tocophenols which cannot be clearly separated by ODS columns.

  
Column : Wakopak® Navi C30-5 (4.6 mm × 250 mm)
Eluent : CH
3CN (100 %)
Flow Rate : 1.0 mL/min. at 35℃
Detection : UV 295 nm, 0.064 AUFS
Injection Volume : 10 μL
Sample:
(1) D-δ Tocophenol (0.1 mg/mL); (2) D-γ Tocophenol (0.1 mg/mL); (3) D-β Tocophenol (0.1 mg/mL); (4) D-α Tocophenol (0.1 mg/mL);

Wakopak® Navi C30-5 with triacontyl groups having excellent structural recognition ability is suitable for analysis of homologs.
(See pages 38 and A-4.)




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