Column Size : 4.6 mm×150 mm
EIuent : CH3CN/50 mM
KH2PO4, 1%Et3N
(pH3.5←H3PO4
=1/9(v/v)
Flow Rate : 1.0 mL/min.
Detection : UV 210 nm
RS is suitable for the separation. In the analysis with polymeric C18 AR, atropine and brucine are dissolved in the reverse order.

EIuent : CH3CN/H2O=60/40(v/v)
Flow Rate : 1.0 mL/min. at 30℃
Detection : UV 254 nm 0.16 AUFS
Sample : 1)Uracil
      2)Benzene
      3)Naphthalene
      4)Biphenyl
      5)Phenanthrene
      6)Fluoranthene
      7)Pyrene
      8)Chrysene
      9)Benzo[a]pyrene
AR shows increased retention of chrysene and benzo[a]pyrene. This is considered to be caused by the space structure recognition ability of AR.

Column Size : 4.6 mm×150 mm
EIuent : A)H2O, 0.1 % TFA
B)CH3CN, 0.1 % TFA
0-15 min, B conc. 10-42 %
15-20min, B conc. 42 %
Flow Rate : 1.5 mL/min. at 35℃
Detection : UV 220 nm
Sample : 1)Arg-Vasopressin
2)Oxytocin
3)Met-Enkephalin
4)Bradykinin
5)Leu-Enkephalin
6)Angiotensin-I
7)Ribonuclease A
8)Insulin
9)Cytochrome C
10)Lysozyme
For this analysis, AR excelling in acid resistance is suitable because it shows favorable shapes of peaks and the eluent includes trifluoroacetic acid.


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