1. Product Outline
Quantitative NMR (qNMR)
NMR (Nuclear Magnetic Resonance) has been broadly used as a standard analysis method for structural determination of an organic compound. In NMR analysis,
the ratio of the number of atomic nuclei in a compound corresponds to the ratio of the areas of the peaks in the spectrum. Therefore, the purity of the compound
can be determined by performing a quantitative analysis.
In a purity determination using 1H NMR, a sample and a standard (an internal standard) having a known purity are mixed together and dissolved in a deuterated
solvent. A quantitative value of the sample’s purity can be calculated from the relationship among the areas of the spectral peaks originated from the sample
and the standard, the number of protons, the prepared masses, and the molecular weights of the sample and the standard using Formula 1.
|In quantitative analysis using 1H NMR (quantitative NMR), the areas of the peaks of hydrogen atoms observed in the spectrum can be
quantitatively compared. Therefore, determination of the purity and quantity of many compounds containing a hydrogen atom is possible with one standard as long
as the signals of the sample and the standard do not overlap each other. Highly reliable purity and quantity measurement based on the amount of substance
(moles) is possible using a standard certified to be traceable to the International System of Units (SI).
Internal Standards for Quantitative NMR
As a producer of internal standards for quantitative NMR, we acquired accreditation by the ASNITE (Accreditation System of the National Institute of Technology
and Evaluation) accreditation program operated by the National Institute of Technology and Evaluation (NITE / IA Japan), and provide the standards as certified
reference materials (TraceSure).
The certification gives an uncertainty value as proof of reliability and is attached to every ASNITE accredited and certified reference standard. The certified
value described in the certification is internationally accepted through a mutual recognition agreement of APLAC (the Asia Pacific Laboratory Accreditation
We determine a characteristic value of the standard by adding uncertainty obtained from our uniformity evaluation, stability evaluation, etc. to the value of
purity (including uncertainty) obtained with a measurement method that is traceable to International System of Units (SI) by the National Metrology Institute of
Japan (NMIJ), and we provide the internal standard for quantitative NMR as a reference standard with a certification. Therefore, the characteristic value of the
reference standard is traceable to SI through the NMIJ analysis value, and the traceability of the measurements can be shown.
NOTE: TraceSure is our series of standards accredited by ASNITE "Reference material producer accreditation" from NITE. ASNITE: Accreditation system of NITE.
Accreditation System of National Institute of Technology and Evaluation.