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for Stevioside Determination
Highly-Purified Steviol Glycosides listed in JECFA Monographs

 

  • Leaflet(141 KB/2 pages)


  • Stevia rebaudiana (Stevia) is a plant native to South America. The leaf of the Stevia plant contains sweet components, called steviol and steviol glycosides which includs Stevioside, Dulcoside A, Rebaudioside A, B, C, D, F and others. The Stevia extract has been listed as a food additive in the 8th edition of Japan's Specifications and Standards for Food Additives.   Furthermore, they are also listed as Steviol Glycosides in JECFA Monographs.  They have been used as sweeteners around the world. Wako can provide the highly purified products and they can be used for determination of the steviol glycosides.

    Stevia rebaudiana Bertoni

    HPLC chart of Rebaudioside A Standard (Wako Cat. #189-02581)
    Stevia Stevia     HPLC chart of Rebaudioside A
    Specification
    Test
    Isosteviol Std.
    Rebaudioside A Std.
    Rebaudioside B Std.
    Rubusoside Std.
    Appearance
    White, crystalline powder
    White, crystalline powder
    White~, crystalline powder ~ powder
    White~, crystalline powder ~ powder
    Solubility
    Pass the test (in 1,4-Dioxane)
    Pass the test (in Water-MeOH)
    Pass the test (in MeOH)
    Pass the test (in MeOH)
    Melting Point
    229 ~ 232 degrees C
    -
    -
    -

    Specific Rotation
    (@20ºC)

    -80.5 ~ -77.5º
    (in EtOH)
    -20 ~ -24º
    (in MeOH)
    Report measured value
    (in MeOH)
    Report measured value
    (in MeOH)
    Loss on Drying
    (for 2 hr.)
    max. 5.0 %
    (@105 ºC)
    max. 5.0 %
    (@110ºC)
    max. 5.0 %
    (@105ºC)
    max. 5.0 %
    (@105ºC)
    TLC test
    Pass the test
    Pass the test
    Pass the test
    Pass the test
    Assay
    min. 99.0 %
    (HPLC, after drying)
    min. 99.0 %
    (HPLC, after drying)
    min. 99.0 %
    (HPLC, after drying)
    min. 98.0 %
    (HPLC, after drying)
    min. 99.0 %
    (Volumetric analysis)
    -
    -
    -

    Other specifications are available in response to your request.


    <NEW> Rebaudioside C (Wako Catalog No. 181-02541 (25 mg))

    Appearance: White, Crylstals - powder
    Assay (HPLC): 94.0 % (the first lot)

    Chemical Structure:
    Rebaudioside C
    CAS No. 63550-99-2
    C44H70O22 = 951.01
    HPLC chart of Rebaudioside C
    HPLC chart of Rebaudioside C

    <HPLC Conditions>
    Column: Wakosil-II 5C18HG 4.6 mm x 250 mm
    Eluent: Phosphate buffer (pH2.6)* : CH3CN = 68 : 32
    Flow rate: 1.0 mL/min at 40°C
    Detection: UV 210 nm
    Sample: 0.1 %  H2O : CH3CN = 7 : 3 (5 μL)

    <NEW> Rebaudioside F (Wako Catalog No. 186-02611 (5 mg))

    Appearance: White, Crylstals - powder
    Assay (HPLC): 78.2 % (the first lot)

    Chemical Structure:
    Rebaudioside F
    CAS No. 438045-89-7
    C43H68O22 = 936.99
    HPLC chart of Rebaudioside F
    HPLC chart of Rebaudioside C
    <HPLC Conditions>
    Column: Wakosil-II 5C18HG 4.6 mm x 250 mm
    Eluent: Phosphate buffer (pH2.6)* : CH3CN = 68 : 32
    Flow rate: 1.0 mL/min at 40°C
    Detection: UV 210 nm
    Sample: 0.05 %  H2O : CH3CN = 7 : 3 (5 μL)

    <NEW> Stevioside Standard (Wako Catalog No. 199-16291 (100 mg))

    Appearance: White, Powder
    Assay (HPLC): min. 99.0 %

    Chemical Structure:
    Rebaudioside F
    CAS No. 57817-89-7
    C38H60O18 = 804.87
    HPLC chart of Rebaudioside F
    HPLC chart of Rebaudioside C
    <HPLC Conditions>
    Column: Wakosil-II 5C18HG 4.6 mm x 250 mm
    Eluent: Phosphate buffer (pH2.6)* : CH3CN = 68 : 32
    Flow rate: 1.0 mL/min at 40°C
    Detection: UV 210 nm
    Sample: 0.1 %  H2O : CH3CN = 7 : 3 (5 μL)

    *: Take 0.78 g of sodium dihydrogenphosphate dehydrate and add water to prepare the 1L solution. Then adjust the pH to 2.6 with Phosphate Acid (1 → 10)
    QUOTATION REQUEST for reagents for Steviol Glycosides  PAGE TOP

    Product List on Steviol Glycoside

    Description Grade
    Wako Cat. No.
    Package Size
    Dulcoside A Standard, 98.0+% (HPLC)
    for Food Analysis
    048-31211
    25 mg
    044-31213
    100 mg
    Isosteviol Standard, 98.0+% (HPLC)(dry basis)
    for Stevioside Determination
    098-05681
    1 g
    Rebaudioside A Standard, 99.0+% (HPLC)
    for Food Analysis
    189-02581
    100 mg
    185-02583
    1 g
    Rebaudioside B Standard, 98.0+% (HPLC)
    for Food Analysis
    188-02551
    25 mg
    184-02553
    100 mg
    Rebaudioside C, 90.0+% (HPLC)
    for Food Analysis
    181-02541
    25 mg
    Rebaudioside Dupdated
    for Food Analysis
    180-02511
    5 mg
    Rebaudioside F
    for Food Analysis
    186-02611
    5 mg
    Rubusoside Standard, 98.0+% (HPLC)
    for Food Analysis
    187-02521
    25 mg
    183-02523
    100 mg
    Steviol Standard, 99.0+% (HPLC)
    for Food Analysis
    192-15701
    25 mg
    198-15703
    100 mg
    Steviolbioside Standard, 98.0+% (HPLC)
    for Food Analysis
    199-15691
    25 mg
    195-15693
    100 mg
    Stevioside Standard
    for Food Analysis
    199-16291
    100 mg

    QUOTATION REQUEST for reagents for Steviol Glycosides  PAGE TOP

    Note

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