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trans-Fatty Acid Analysis

 

It is known that trans-fatty acid is produced by hydrogenation, etc., in the process of manufacture of hydrogenated oils such as margarine or shortening, etc.

There are reports that the action of trans-fatty acid increases LDL cholesterol, known as bad cholesterol, and that continuing to ingest large quantities of trans-fatty acid increases the risk of ischemic heart disease due to arteriosclerosis, etc.  Against this background, there are moves to investigate making it obligatory to label the content of trans-fatty acid in food in Japan too.
Capillary gas chromatography has been adopted as the provisional method * for the measurement of trans-fatty acid content in JOCS Standard Methods for the Analysis of Fats, Oils and Related Materials.

We handles the trans- substances methyl linolenate and α-methyl linolenate, which are useful in confirming the detection position of trans -fatty acids.

* Reference: JOCS Standard Methods for the Analysis of Fats, Oils and Related Materials: Provisional method 17-2007 trans-fatty acid content (capillary gas chromatography)


1. C18 trans-Fatty Acid Methyl Esters

Example of analysis


Description
Grade
Wako Cat. No. (Pkg. Size)
Methyl 9,12-Octadecadienoate Standard (mixture of cis- and trans-)
[trans-Methyl linoleate]
for Food Analysis
133-16271 (200 mg)
Methyl 9,12,15-Octadecatrienoate Standard (mixture of cis- and trans-)
[trans-Methyl α-linolenate]
for Food Analysis
130-16281 (200 mg)

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2. Related Products
· Internal Standard
Description Grade Wako Catl No. (Pkg. Size)
Heptadecanoic Acid Standard
(98.0+ % (Volumetric analysis); 98.0+ % (cGC))
for Food Analysis
084-08661 (100 mg)

· Methyl Esterizing Agents
Description Grade Wako Cat. No. (Pkg. Size)
Boron Trifluoride Methanol Complex Methanol Solution
About 14-15% (BF3)
for Gas Chromatography
029-06172 (25 g)
021-06171 (400 g)

· Fatty Acid Methyl Esters
Description C Grade Wako Cat. No. (Pkg. Size)
<Saturated Fatty Acid Methyl>
Methyl Palmitate Standard
(98.0+% (Volumetric analysis); 98.0+ % (cGC))
16
for Gas Chromatography
135-07963 (1 g)
139-07961 (5 g)
Methyl Stearate Standard (99.5+ % (cGC))
18
for Gas Chromatography
136-07971 (5 g)
Methyl Arachidate  (95.0+ % (GC))
20
Wako Chemical, Ltd.
325-92291 (5 g)
323-92292 (25 g)
<Mono Unsaturated Fatty Acid (cis-)>
Methyl Oleate Standard (9-cis) (99.0+% (cGC))
18
for Gas Chromatography
139-08583 (1 g)
<Highly-Unsaturated Fatty Acid (cis-)>
Methyl 9,12-Octadecadienoate Standard
(mixture of cis- and trans-)
[trans-Methyl Linoleate]
18
for Food Analysis
133-16271 (200 mg)
Methyl 9,12,15-Octadecatrienoate Standard
(mixture of cis- and trans-)
[trans-Methyl α-Linolenate]
18
for Food Analysis
130-16281 (200 mg)

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