PROPOSAL from WAKO
How about adopting Highly-Purified products to satisfy
Universal Qualifying Standards for Your Analysis?
Universal Standard (USP/EP/JP) Reagents
suitable in high-performance Liquid Chromatography
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To meet each specifications of JP (Reagents), USP (Reagents) and EP (Reagents)
Wako’s acetonitrile, methanol and hexane for the Japanese Pharmacopoeia (JP) General Tests (for Liquid
Chromatography) can meet not only corresponding specifications of reagents of the JP but also those of the
USP, which corresponds to the specifications of the current edition of ACS Reagent Chemicals as well as those
of the European Pharmacopoeia (EP). Such high-grade reagents achieve to analyze drugs test at every
laboratory of the world.
Features:
1.






2.


3.


4.
Meet the following specifications listing on
“Reagents" of one or all of the latest JP, USP and
EP:




Samples are highly stable in the solution with
these highly-purified solvents.

Achieve to reduce the probability that the filter or
line are clogged

Highly chromatographic-sensitive and
reproducible
Water; Residue after evaporation; Refractive
Index; UV absorbance; Fluorescent test;
Peroxides, etc..
▶ for the Japanese Pharmacopoeia General Tests (for Liquid Chromatography)
Description
Wako Catalog No.
Pkg. Size
019-21691
1 L
Acetonitrile
99.9+ % (EP method); 99.8+ % (cGC); 99.5; % (USP-NF method)
015-21693
3 L
136-15661
1 L
Methanol ,99.8+ % (USP-NF method; EP method); 99.7+ % (cGC)
132-15663
3 L
085-08711
1 L
Hexane ,98.5+ % (USP-NF method); 96.0+ % (cGC)
081-08713
3 L
Prices are shown on the online catalog.
Please visit the following: https://www.e-reagent.com
Acetonitrile Tested Item Wako Product Specifications of Acetonitrile as a reagent on each Pharmacopoeia
USP grade
(ACS reagent grade)
EP grade JP grade
General Use
1 Assay 99.9+ % 99.5+ % 99.9+ % 99.8+ %
2 Appearance Colorless, clear
liquid
Clear, colorless liquid Clear, colorless liquid Clear, colorless liquid
3 Color(APHA) max. 10 max. 10 - -
4 Refractive Index 1.343 ~ 1.346 - 1.344 -
5 Residue after
evaporation
max. 0.001 % max. 0.005 % - -
6 Titrable acid max. 8 μeq/g max. 8 µeq/g - -
7 Titrable base max. 0.6 μeq/g max. 0.6 μeq/g - -
8 Warer (H2O) max. 0.05 % max. 0.3 % - -
9 pH A 100 g/L solution is
neutral to litmus
paper
- A 100 g/L solution is
neutral to litmus
paper
-
10 Distillation Range
(80 ~ 82 ºC)
min. 95 % - min. 95 % -
Specific Use
UV Spectrophotometry
11 Wavelength
(maximum allowable
Absorbance (AU))
190nm   1.00
200nm   0.05
210nm   0.03
220nm   0.02
230nm 0.014
240nm 0.009
190 nm   1.00


220 nm   0.05


254 nm 0.01

200nm   0.10
200nm   0.07  
210nm   0.046
220nm   0.027
230nm   0.014
240nm   0.009
Liquid Chromatography Suitability
12 Gradient elution To pass test Passed test - -
13 Transmittance (240 nm) min. 98 % - min. 98 % -
Specifications added by Wako
14 Ammonium max. 0.3 ppm - - -
15 Peroxides max. 5 ppm - - -
16 Substances
Reducing
Permanganate
To pass test - - -
17 Fluorescence Test To pass test - - -