Wako Pure Chemical Industries, Ltd.Wako Pure Chemical Industries, Ltd. - Laboratory Chemicals
Home > Products > Analysis > General analysis  >  XenoScreen™ GSH-EE

Reagent for Reactive Metabolites Detection
XenoScreen™ GSH-EE  (Tube type)


XenoScreen™ GSH-EE (Tube type) is useful for identification of candidate drug compounds that produce reactive metabolites. Reactive metabolites are metabolites with high reactivity, generated by metabolism of a candidate drug compound, and are considered to be involved in the occurrence of idiosyncratic drug toxicity [IDT].


1. Features


  • Sensitive and selective
    Reactive metabolites can be detected with high-sensitivity in comparison with glutathione, by using glutathione ethyl ester (GSH-EE) as a trapping reagent.
    Detection can be performed easier by using GSH-EE D-isomer together.

  • Ready to use reagent (No reagent preparation is required.
    For one reaction, essential trapping reagents and various buffers can be aliquoted in multiple tubes. Troublesome weighing out of reagents and preparation of stock solutions are not necessary.

2. Reaction



Reaction

3. Analysis



Reaction

4. References


  1. Ma, L., Wen, B., Ruan, Q. and Zhu, M.: Chemical Research in Toxicology. 21, 1477 (2008).
  2. WO 2012/160798

5. Product List


Product Name
Pkg. Size
Wako Cat. No.
Grade
Storage Condition
XenoScreen™ GSH-EE(Tube type)
12 each
for Pharmacokinetics Research
Keep at -20°C.



6. Related Products



Presep®XenoScreen™ 96-well plate is a hydrophlic reverse-phase polymer packed solid-phase sample plate.   For pharmacokinetic studies, the plate may be used for the removal of impurities or for the concentration of constituents of reactive metabolites.

Related products

Usage

Usage


Product Name
Pkg. Size
Wako Cat. No
Grade
Storage
Condition
Presep® XenoScreen™ 96-well plate
1 pack
for Pharmacokinetics Research
Keep at room temperatire

Other than the XenoScreen™ plate, the following reagents and analytical column are required.

Product Name
Pkg. Size
Wako Cat. No.
Grade
Storage Condition
Nicotinamide Adenine Dinucleotide Phosphate, Reduced Form
(β-NADPH) [β-NADPH-Na4]
1 g
Oriental Yeast Co., Ltd.
Keep at -20°C
(±)-Dithiothreitol [DTT] (97.0+ %)
1 g
for Molecular Biology
Keep at 2-10°C
Acetonitrile (99.8+ % (cGC))
1 L
for HPLC
Keep at room temperature.
Formic Acid (99.5+ % (HPLC))
25 mL
Keep in a dark place.
Methanol (99.7+ % (cGC))
1 L
Keep at room temperature.
Wakopak® Ultra C18-2 φ 2.1 mm x 100 mm (W)
1 pack
for Ultra HPLC

NOTE: For the latest price information, access Wako Online Catalog with a click of each Wako Catalog No.

Home > Products > Analysis > General analysis  >  XenoScreen™ GSH-EE