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Home > Products > Life Science > 1x Buffer Series

Ready-to-use all-purpose Buffers
1x Buffer Series

The buffers in this series do not require dilution and are ready to use.   The buffers are offered in sterile 5 L containers with capped stopcocks.

  • Package size: 5 L
  • Dimensions: 20 x 20 x 20 (cm)
  • Accessories: A capped stopcock

  1. Instructions for use
  2. Compositions of Buffers
  3. Product List

1. Instructions for use

  1. Open the box along the circular cutout line on the top and remove the capped stopcock.
  2. Gently pull the cap on the container upward until it comes out of the box.
  3. Replace the container cap with the capped stopcock.
  4. Reposition the box so the spout of the stopcock faces downward. Open the stopcock to collect buffer.

2. Compositions of Buffers

  • 1 x PBS(-)
    Free of calcium or magnesium ions
    Composition:137 mmol/L NaCl, 8.1 mmol/L Na2HPO4, 2.68 mmol/L KCl, 1.47 mmol/L KH2PO4, pH 7.4

  • 1 x PBS(-)-T
    Free of calcium or magnesium ions. Treated with Tween-20.
    Composition:137 mmol/L NaCl, 8.1 mmol/L Na2HPO4, 2.68 mmol/L KCl, 1.47 mmol/L KH2PO4, pH 7.4, 0.1 w/v% Tween-20

  • 1 x TBS
    Composition:137 mmol/L NaCl, 2.68 mmol/L KCl, 25 mmol/l Tris-HCl, pH 7.4

  • 1 x TBS-T
    Treated with Tween-20.
    Composition:137mmol/L NaCl, 2.68 mmol/L KCl, 25 mmol/L Tris-HCl, pH7.4, 0.1 w/v% Tween-20

  • 1 x TAE
    A running buffer for preparing agarose gel and conducting agarose gel electrophoresis.
    Composition:20 mmol/L Acetic Acid, 1 mmol/L EDTA, 40 mmol/L Tris-HCl, pH 7.4

  • A running buffer for SDS-PAGE
    Composition:192 mmol/L Glycine , 0.1 w/v% SDS, 25 mmol/L Tris-HCl, pH 8.5

3. Product List
Product Name Grade Package Size Wako Catalog No.
1 x PBS(-) for Biochemistry 5 L 164-25511
1 x PBS(-)-T for Biochemistry 5 L 161-25521
1 x TBS for Biochemistry 5 L 209-19121
1 x TBS-T for Biochemistry 5 L 206-19131
1 x TAE for Genetic research 5 L 203-19141
SDS-PAGE Buffer, pH8.5 for Electrophoresis 5 L 192-16801


  • Listed products are intended for laboratory research use only, and not to be used for drug, food or human.
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Home > Products > Life Science > 1x Buffer Series