2-Mercaptoethanol is in extensive uses including protection of
SH group in a protein, breakage of disulfide bond and extraction
of crude proteins. However, 2-mercaptoethanol and preparations
containing it were designated to poisons by the revision of the
Poisonous and Deleterious Substances Control Act in Japan in 2008
and control has been tightened. 3-Mercapto-1,2-Propanediol
is a new reducing agent replacing 2-mercaptoethanol.
It has been confirmed to show reducing potential equal to 2-mercaptoethanol
by the SDS-PAGE. Its DNase and RNase activities have already
been confirmed as a product of molecular biological grade.