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Low-molecular compound for cell proliferation/cell adhesion promotion
Adhesamine

Adhesamine is a low-molecular compound that promotes adhesion of cells to a culture container as well as cell proliferation. It exhibits fibronectin-like cell adhesion activity through binding to heparan sulfate on the cell surface.  It allows adhesion of floating cells to a culture container by coating the container or adding to a medium.   The cells adhered to the container by this product are freed from the container when the medium is replaced with one not containing this product. Its effect has been demonstrated with HeLa, HEK293, CHO and mouse ES cells 1). It has also been reported that addition of this product to a medium improves the proliferation of cells that are difficult to culture2).

⇒ 1. Features
⇒ 2. Example of use (i): Coating
⇒ 3. Example of use (ii): Addition to a medium
⇒ 4. Precautions for use
⇒ 5. References
⇒ 6. Product List

Package Insert


1. Features
  • Exhibits fibronectin-like cell adhesion activity.
  • A synthetic product with little risk of infections and inter-lot differences.
  • Promotes cell adhesion and cell proliferation.
  • Apllicable as coating or an additive to media.
  • CAS No.: 462605-73-9
  • C24H32Cl4N8O2S2 = 670.51



2. Example of use (i): Coating

  1. Dissolve 10 mg of Adhesamine in 1 mL of DMSO (by sonication for about 5 minutes).
  2. Use the solution prepared in 1 as the stock solution and prepare diluents of 10, 25, 50 and 100 μg/mL using PBS (-).
  3. Distribute 500 μL each of adhesamine solutions of respective concentrations in a 24-well plate and incubate at 37°C for 1 hour.
  4. Aspirate the adhesamine solutions and wash the wells with PBS(-).
  5. Seed 0.5 x 106 cells (500 μL) of Jurkat cells in each well.
  6. Incubate at 37°C in 5% CO2 for 5 hours. → Below figure.
  7. Wash with PBS(-).
  8. Fix the cells with 4% paraformaldehyde.
  9. Stain the cells with 5 mg/mL crystal violet.
  10. Wash away excess crystal violet and dry.
  11. Elute crystal violet with 2% SDS solution and determine at OD550. → Right figure.



3. Example of use (ii): Addition to media

  1. Dissolve 10 mg of Adhesamine in 1 mL of DMSO (by sonication for about 5 minutes).
  2. Use the solution prepared in 1 as the stock solution and prepare diluents of 0.5, 1.0, 2.5 and 5.0 mg/mL.
  3. Distribute 5 μL each of Adhesamine solutions of respective concentrations in a 24-well plate.
  4. Seed 0.5 x 106 cells (500 μL) of Jurkat cells in each well.
  5. Incubate at 37 °C in 5% CO2 for 5 hours. → Below figure.
  6. Wash with PBS(-).
  7. Fix the cells with 4% paraformaldehyde.
  8. Stain the cells with 5 mg/mL crystal violet.
  9. Wash away excess crystal violet and dry.
  10. Elute crystal violet with 2% SDS solution and determine at OD550. → Right figure.


4. Precautions for use
Precipitation may occur by storage as a solution after dissolution in DMSO. Preparation just before use is recommended as precipitation, if occurred, may not be redissolved.



5. References
1) Yamazoe, S., et al.:, Chem. Biol., 16, 773 (2009).
2) Hoshino, M., et al.:, Biochem. J., 427, 297 (2010).


6. Product List
Description Grade Package .Size Wako Catalog .Number
Adhesamine for Cell Culture 1 mg 010-23201
Products listed in this page are for research use only.  Do not administer each to human.



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