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Research for Diabetes and Oxidative Stress
Aldose Reductase , Human, recombinant, expressed in Insect Cells
Wako Catalog No. 012-23761 (0.4 units)

Aldose Reductase catalyzes the first step in the polyol pathway and is implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Furthermore, this enzyme may have other activities that intersect with signal processing and oxidative defense.

Product Data

  • Description
    Aldose Reductase (Code No.012-23761) from human muscle cell is purified from the culture medium of baculovirus-insect cell expression system. cDNA containing the entire protein coding region of human aldose reductase is expressed and the enzyme retains the same properties exhibited by human muscle and retina.
  • Source
    Human aldose reductase expressed in insect cells
  • Molecular Weight: Approximately 34 kDa
  • Purity: After SDS-PAGE with 1 μg of the enzyme, appeared as a main band of approximately 34kDa by Coomassie brilliant blue staining (Figure 1).
  • Form &: Buffers
  • Activity: 1.0 ± 0.4 units/mL
  • Specificy Activity: 1.4 ± 0.4 units/mg protein
  • Unit Definition:
    One unit is defined as the amount of enzyme required for depleting 1 μmol of NADPH per minute.
  • Storage: -20 °C
  • EC No.: 1.1.1.21
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Figure 1: SDS-PAGE with 1 μg of
Aldose Reductase
Lane 1: MW size marker
Lane 2: Aldose Reductase

Role of Aldose Reductase in Polyol Pathway


Aldose Reductase normally has the function of reducing toxic aldehydes in the cell to inactive alcohols, but when the glucose concentration in the cell becomes too high, aldose redutase also reduces that glucose to sorbitol, which is later oxidized to fructose. In the process of reducing high intracellular glucose to sorbitol, the aldose reductase consumes the NADPH. By reducing the amount of NADPH, the polyol pathway increases susceptibility to intracellular oxidative stress.


References


  1. Brownlee M.: "The pathology of Diabetic Complications" Diabetes., 54, 1615-24, (2005).
  2. Petrash JM: "All in the family: aldose reductase and closely related aldo-keto reductases", Cell. Mol. Life Sci., 61 (7-8), 737-49, (2004)
  3. Schrijvers BF, De Vriese AS, Flyvbjerg A.: "From hyperglycemia to diabetic kidney disease: the role of metabolic, hemodynamic, intracellular factors and growth factors/cytokines", Endocr. Rev. 25(6): 971-1010 (2004).

 

Product List


Wako Catalog No. (Package Size) Product Name Grade
012-23761 (0.4 units) Aldose Reductase for Cellbiology

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