For immunoprecipitation
Anti Ago1,
Monoclonal Antibody
(Clone No. 2A7 & 1F2)
(Clone No. 2A7: Wako Cat. No.015-22411 (50µL); Clone No. 1F2: Wako Cat. No. 018-22401 (50µL))
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Argonaute1 (Ago1) was isolated as one of the components of RISC (RNA-induced
silencing complex). This monoclonal antibody is used for immunoprecipitation (IP) of
Ago1 protein and microRNA interacted with Ago1.
Features
1) For IP
2) Cross reactivity with Ago1 protein of mouse and human
3) For purification of RNA captured by RISC
INFORMATION
Concentration(protein) : Indicated on the label.
(1st Lot. 1.0mg.mL)
Formulation : 0.05% Sodium Azide,
10% Glycerol with 1xTBS, pH7.4.
Clone No. : 2A7
Subclass : IgG2aK
Antigen : Synthesized peptide of N terminal of Ago1.
Strage : 2~10ºC in the dark.
[Working Dilution]
Application
Working Dilution
Immunoprecipitation
5-10µg/20µL
10% Protein G slurry
[Cross Reactivity]
Organisms
Human
Mouse
Cell
HeLa
FM3A
Immunoprecipitation
microRNA Purification
APPLICATION DATA
Immunoprecipitation of endogenous Ago1 (Cell)
microRNA purification from
from endogenous Ago1 IP fraction (Cell)
Figure 1
Immunoprecipitation of Ago1 protein from HeLa and FM3A cell
line by using 20µL of 10% Protein G slurry immobilized with 5µg
of Anti Ago1, MAb (2A7). The band of endogenous Ago1 protein
was detected in approximately 98kDa by Western blot analysis.
The 1/200 diluted this antibody was used as the 1st antibody*1
for Western blot. Cell number was 1107.
*1 : Now developing. Coming soon.
Figure 2
microRNA purification from Ago1 IP fraction of HeLa and FM3A
cell line by using 20µL of 10% Protein G slurry immobilized with
5µg of Anti Ago1, MAb (2A7). The purified microRNA fraction were
specifically detected by silver stain after Urea-PAGE. Cell number
was 2107.
Immunoprecipitation of
endogenous Ago1 (Mouse Tissue)
microRNA purification
from endogenous Ago1 IP fraction (Mouse Tissue)
Figure 3
Immunoprecipitation of Ago1 protein from lung and brain tissue
(15mg each) of mouse by using 20µL of 10% Protein G slurry
immobilized with 5µg of Anti Ago1, MAb (2A7). The band of
endogenous Ago1 protein was detected in approximately 98kDa
by Western blot analysis with Anti Ago1, MAb (1F2).
Figure 4
microRNA purification from Ago1 IP fraction of lung and brain
tissue (30mg each) of mouse by using 20µL of 10% Protein G
slurry immobilized with 5µg of Anti Ago1, MAb (2A7). The purified
microRNA fraction were specifically detected by silver stain after
Urea-PAGE.
Code No.
Description
Contents
015-22411
014-22023
018-22021
011-22033
015-22031
292-66701
292-67301
290-66501
298-65103
292-65101
Anti Ago1, Monoclonal Antibody (2A7)
Anti Mouse Ago2, Monoclonal Antibody
Anti Human Ago2, Monoclonal Antibody
microRNA Isolation Kit, Human Ago2
microRNA Isolation Kit, Mouse Ago2
microRNA Cloning kit Wako
Single Strand DNA Ligase,
thermostable, recombinant, Solution
50 µL
50 µL
100 µL
50 µL
100 µL
10 reactions
10 reactions
8 reactions
200 units
500 units
Listed products are intended for laboratory research use only, and not to be used for drug, food or human.
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