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For Alzheimer’s Disease Research
Anti Amyloid β Antibody BAN50 / BA27 / BC05

Amyloidβ(Aβ) is a peptide which consists of about 40 amino acids. Aβ is considered the cause of Alzheimer’s disease, because it is a major component of senile plaques formed in the brains of Alzheimer’s disease patients. Aβ has several types differing in length of the amino acid. The main types are Aβ40 (40 amino acid) , Aβ42(42 amino acid), and Aβ43(43 amino acid). This items are mouse monoclonal antibodies developed by Takeda Pharmaceutical Company Limited.


1. LINK

2. Features

  • High reactivity and specify
  •  

  • 3 kinds of antibodies
     BAN50 :Human Aβ N-terminus specific
     BA27  :Aβ40 C-terminus specific
     BC05  :Aβ42/43 C-terminus specific
  •  

    Clone No. Isotype Applications Concentration Reactivity
    Human Mouse/ Rat
    Aβ40 Aβ42 Aβ43 Aβ40 Aβ42 Aβ43
    BAN50 Mouse IgG1・κ WB / IP / IHC / ICC / ELISA 0.9-1.3mg/mL
    BA27 Mouse IgG2a・κ WB / IHC / ELISA 0.9-1.3mg/mL
    BC05 Mouse IgG1・κ WB / IHC / ELISA 0.9-1.3mg/mL

     

  • Abundant reference
    1) Asami-Odaka, A., et al.: Biochemistry., 34, 32, 10272(1995).
    2) Takahara, Y., et al.: J. Neurosci., 25, 2, 436(2005).
    3) Iwata, H., et al.: J. Biol. Chem., 276, 24, 21678(2001).
    4) Tomita, T., et al.: J. Neurosci., 19, 24, 10627(1999).
    5) Sudoh, S., et al.: J. Neurochem., 71, 4, 1535(1998).
    6) Iwatsubo, T., et al .: Am J. Pathol., 149, 6, 1823(1996). etc.

3. Immunohistochemistry -brain in Alzheimer’s disease model mouse-

Application-Immunohistostaining -

                        →Aggregated Aβin model mouse brain are immunostained by using this antibodies.

Assay condition
Sample

Paraffin section of piriform cortex in Alzheimer’s disease model mouse brain 15-month old

B6.Cg-Tg(APPswe.PSEN1dE9)85Dbo/Mmjax

Antigen retrieval BAN50:heat treatment in citrate buffer(pH6.0) and proteinase K treatment
BA27/BC05:formic acid treatment
Antibody concentration BAN50/BA27:1/1,000
BC05 :1/100,000
Secondary antibody Biotin-conjugated anti mouse IgG

Detection

method

ABC +DAB

All immunohistochemistry data were provided by Konakawa, A., Hashimoto, T., and Iwatsubo,T., Graduate School of Medicine, University of Tokyo in Japan.

4. Western blotting – brain lysate of Alzheimer’s model mouse-

■Detection of insoluble Aβ

Insoluble Aβ in model mouse brain are detected by using the antibodies.

Assay condition
Sample

Lane 1-3: Formic acid -extracted DMSO solution of wildtype mouse brain, 15-month old

Lane 4-6: Formic acid -extracted DMSO solution of Alzheimer’s disease model mouse brain,15-month old B6.Cg-Tg(APPswe.PSEN1dE9)85Dbo/Mmjax

Reduction treatment done
Apply 10μL/Lane
Antibody concentration 1/1,000

 

■Detection of soluble Aβ

Assay condition
Sample

Lane 1-3: TBS lysate of wildtype mouse brain , 15-month old

Lane 4-6: TBS lysate of Alzheimer’s disease model mouse brain ,15-month old B6.Cg-Tg(APPswe.PSEN1dE9)85Dbo/Mmjax

Apply 30μg/lane
Reduction treatment done
Antibody concentration 1/1,000

 

Soluble Aβ in model mouse brain are detected by using the antibodies.

All Western blotting data were provided by Hashimoto, T., and Iwatsubo,T., Graduate School of Medicine, University of Tokyo in Japan.

5. Product List

Product Name Pkg. Size Wako Cat. No. Storage Condition
Anti Human Amyloid β, Monoclonal Antibody
(BAN50)
10μL
50μL

017-26871

013-26873

Keep at -20°C
Anti Amyloid β40, Monoclonal Antibody
(BA27)
10μL
50μL

018-26921

014-26923

Anti Amyloid β42(43), Monoclonal Antibody
(BC05)
10μL
50μL
014-26901
010-26903
 
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