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FUJIFILMFUJIFILM Wako Pure Chemical Corporation Laboratory Chemicals
Home  > Products >  Life Science > Genetic Research  >  Anti-CD63, monoclonal antibody (3-13)

High-sensitive detection of human CD63 protein on exosome surface
Anti-CD63, monoclonal antibody (3-13)

Exosomes were purified from COLO201 cell culture supernatant and used for comparison of detection sensitivity of Western Blotting.

This product was capable of detecting CD63 protein on exosome surface at a sensitivity higher than those of Competitors A and B.
 
Exosome isolation MagCapture™ Exosome Isolation Kit PS (Code No. 293-77601)
Gel SuperSep™Ace 5-20%, 17 wells (Code No. 194-15021)
Running buffer SDS-PAGE Buffer, pH8.5 (Code No. 192-16801)
Blocking 3% Skimmed milk/PBS-T
Chemiluminescent reagent ImmunoStar® Zeta (Code No. 295-72404)
Primary antibody diluted 1,000-fold
Secondary antibody anti-mouse IgG (H+L, peroxidase-conjugated, diluted 10,000- fold) (for Competitor B, the secondary antibody included in the product was used)

 


1. LINKS

2. Features

■High-sensitive detection
■Low cost
■Specific to human CD63*1
*1 Does not recognize mouse, rat, and bovine CD63.

3. Antibody information

■ Cross-reactivity: Human
■ Application WB: 1:500 - 1:2,000 (non-reducing condition) ELISA: 1:1,000-1:16,000 FCM: 1:100-1:1,000
■ Solvent composition: 1×TBS, 50% glycerol, 0.05 w/v% sodium azide
■ Antibody concentration: 1.1 mg/mL
■ Immunized animal: Mouse
■ Subclass: IgG1

4. Product Lists

Product Name Pkg. Size Wako Cat. No. Storage Condition
Anti-CD63, monoclonal antibody (3-13)

20 μL

100 μL

016-27061

012-27063

Keep at - 20°C
Home  > Products >  Life Science > Genetic Research  >  Anti-CD63, monoclonal antibody (3-13)