Antibody against Macrophage/Macroglia-specific Protein, Iba1
Anti Iba1, Polyclonal Antibody, Rabbit
for Immunocytochemistry: Cat. #019-19741 (50 µg (100 µL); for Western Blotting: Cat. #016-20001 (50 µg (100 µL)
Features
Specific to microglia and macrophages but not cross-reactive with neurons and astrocytes
Reactive with human, mouse and rat Iba1
Iba1: ionized calcium binding adapter molecule 1
⇒ Application
for Paraffin section
Application
Immuno-double staining of rat primary mixed culture cells
Green: Iba1, which reacts to anti Iba1 antibody (Wako Cat. #019-19741)
Red: Astrocyte, which reacts to anti GFAP, mAb
(Data was provided by Dept. of Neurochemistry, National Institute of Nueroscience (Japan))
Iba1
Calcium ions are known to be one of the most important signal mediators in all cells including central nervous system (CNS) cells.
Calcium ions exert their signaling activity through asociation with various calcium binding proteins, many of which are classified
into a large protein family, the EF hand protein family.
Iba1 is a 17-kDa EF hand protein that is specifically expressed in macrophages/microglia and is upregulated during the activation
of these cells.
Wako has launched rabbit polyclonal antibodies were raised against a synthetic peptide corresponding to the Iba1 carboxy-terminal
sequence, which was conserved amoung human, rat and mouse Iba1 protein sequences. The antibody for
immunocytochemistry is specifically reactive to microglia/macrophages, is appropriate for immuno-double staining of brain tissues
and cell culture in combination with monoclonal antibody to GFAP (Glial Fibrillary Acidic Protein), which specifically reacts to
astorocyte.
