Wako Pure Chemical Industries, Ltd.Wako Pure Chemical Industries, Ltd. - Laboratory Chemicals
Home > Products > Life Science > ImmunoChemistry  >  Anti Iba1, Rabbit

Microglia-specific antibody
Anti Iba1, Rabbit


Iba 1 is a calcium-binding protein with a molecular weight of 17,000 specifically expressed in macrophage/microglia.
Recently, microglia has attracted attentions because in addition to its role in neurotrophic effects/neuroprotective actions, neurological damage effect by production of NO, TNF-α, and IL-1β have been also proved.
Since this product is a rabbit polyclonal antibody that specifically reacts with microglia, it is appropriate for a double stain with anti-GFAP monoclonal antibody specific to astrocytes, for example.




1. LINK to Leaflets



Leaflet · Anti Iba1 Link to Leaflet (1.2 MB / 2p)
Leaflet · Labeled Anti Iba1 Link to Leaflet (1.5 MB / 2p)
Leaflet ·Monoclonal Antibodies Link to Leaflet (1.2 MB / 2p)

2. Features



  1. Antigen: Synthetic peptide corresponding to C-terminus of Iba1
  2. Form: TBS Solution (1 mg/mL)
  3. Purification: Purified by the antigen affinity chromatography from rabbit antisera
  4. Specificity:
              Specific to microglia and macrophage, but not cross-reactive with neuron and astrocyte.
              Reactive with human, mouse an rat Iba1.
  5. Usage:
              1. Anti Iba1, Rabbit (for immunocytochemistry) is appropriate for immunocytochemistry. It can be used at 1 to 2 μg/mL
              2. Anti Iba1, Rabbit (for Western blotting) is appropriate for Western blotting. It can be used at 0.5 to 1 μg/mL.



3. Images of a double stain and a phase contrast of the same field in rat primary mixed culture cells



Images of a double stain and a phase contrast of the same field in rat primary mixed culture cells

4. Western blotting



Western blotting


5. Recommended Protocol


Anti Iba1 for Immunocytochemistry (Wako Cat.#019-19741)

  a) Application for paraffin Sections

  1. Deparaffinization
    100 % xylene for 5 min, 3 times
    100 % ethanol for 5 min, 2 times
    90 % ethanol for 2 min
    70 % ethanol for 2 min
    dH2O, 3 minutes
  2. Antigen retrieval
    10mM citrate buffer(pH6.0), 90°C, 9min
  3. Wash with PBS, 5 min, 2 times.
  4. Incubate with blocking buffer (2.5% normal goat serum in PBS), 20 min, at room temperature (RT).
  5. Wash with PBS, 5 min, 2 times.
  6. Incubate with anti Iba1 Antibody (1:1,000)(Wako Catalog No. 019-19741) in the blocking buffer overnight at 4°C.
  7. Wash with PBS, 5 min, 3 times.
  8. Incubate with Alexa Flour 488, anti-Rabbit IgG Antibody (1:200) in the blocking buffer for 2hours at RT.
  9. Wash with PBS, 5 min, 3 times.
  10. DAPI staining
  11. Mount



  b) Application frozen sections

  1. Rat or mouse was perfusion-fixed with 4% paraformaldehyde,
    replaced sucrose, and prepared 50μm brain section by microtome.
  2. Wash with 0.3% TritonX-100 in PBS, 5 min ×3.
  3. Incubate with blocking buffer(1 % BSA, 0.3 % TritonX-100/PBS), 2hours,RT.
  4. Incubate with Anti Iba1 Antibody (1:1,000)(Wako Catalog No. 019-19741) in the blocking buffer(1 % BSA, and 0.3 % TritonX-100 in PBS), over night, 4°C.
  5. Wash with 0.3% TritonX-100 in PBS, 5 min ×3.
  6. Incubate with AlexaFlour488 anti-rabbit IgG(1:200) in the blocking buffer(1% BSA, and 0.3 % TritonX-100 in PBS), 2hour,RT.
  7. Wash with 0.3% TritonX-100 in PBS, 5 min ×3.
  8. Mount

6. References



  1. Imai, Y., Ibata, I., Ito, D., Ohsawa., K. and Kohsaka, S.: Biochem. Biophys. Res. Commun., 224, 855(1996).
  2. Ito, D., Imai, Y., Ohsawa, K., Nakajima, K., Fukuuchi, Y. and Kohsaka, S.: Brain Res. Mol. Brain Res., 57, 1(1998).
  3. Ohsawa, K., Imai, Y., Kanazawa, H., Sasaki, Y. and Kohsaka, S.: J. Cell Sci ., 113, 3073 (2000).
  4. Sasaki, Y., Ohsawa, K., Kanazawa, H., Kohsaka, S. and Imai, Y.: Biochem. Biophys. Res. Commun ., 286, 292(2001).
  5. Kanazawa, H., Ohsawa, K., Sasaki, Y., Kohsaka, S. and Imai, Y.: J. Biol. Chem., 277, 20026 (2002).


  6. ⇓ References related on Anti Iba1 published in 2012:

  7. Ziehn, Marina O., Andrea A. Avedisian, Shannon M. Dervin, Elizabeth A. Umeda, Thomas J. O'Dell and Rhonda R. Voskuhl, "Therapeutic Testosterone Administration Preserves Excitatory Synaptic Transmission in the Hippocampus During Autoimmune Demyelinating Disease." Journal of Neuroscience, 32 (36) (Sep. 5, 2012): 12312-24.
  8. Lavisse, Sonia, Martine Guillermier, Anne-Sophie Herard, Fanny Petit, Marion Delahaye, Nadja Van Camp, Lucile Ben Haim, et al., "Reactive Astrocytes Overexpress TSPO and Are Detected by TSPO Positron Emission Tomography Imaging.", Journal of Neuroscience, 32 (32), (Aug. 8, 2012): 10809-18.
  9. Shields, Shannon D., Xiaoyang Cheng, Nurcan Uceyler, Claudia Sommer, Sulayman D. Dib-Hajj, and Stephen G. Waxman. "Sodium Channel Nav1.7 Is Essential for Lowering Heat Pain Threshold After Burn Injury", Journal of Neuroscience, 32 (32), (Aug. 8, 2012): 10819-32.
  10. Lalancette-Hebert, Melanie, Vivek Swarup, Jean Martin Beaulieu, Ivan Bohacek, Essam Abdelhamid, Yuan Cheng Weng, Sachiko Sato, and Jasna Kriz., "Galectin-3 Is Required for Resident Microglia Activation and Proliferation in Response to Ischemic Injury.", Journal of Neuroscience, 32(30), (July 25, 2012): 10383-95.
  11. Kim, Doo-Sik, Kang-Wu Li, Amin Boroujerdi, Yanhui Peter Yu, Chun-Yi Zhou, Ping Deng, John Park, et al. "Thrombospondin-4 Contributes to Spinal Sensitization and Neuropathic Pain States", Journal of Neuroscience, 32(26), (June 27, 2012): 8977-87.
  12. Oluich, Laura-Jane, Jo Anne S. Stratton, Yao Lulu Xing, Sze Woei Ng, Holly S. Cate, Pankaj Sah, Francois Windels, Trevor J. Kilpatrick, and Tobias D. Merson, "Targeted Ablation of Oligodendrocytes Induces Axonal Pathology Independent of Overt Demyelination", Journal of Neuroscience, 32(24) (June 13, 2012): 8317-30.
  13. Chen, Bo, Beth Friedman, Michael A. Whitney, Jessica A. Van Winkle, I-Farn Lei, Emilia S. Olson, Qun Cheng, et al. "Thrombin Activity Associated with Neuronal Damage During Acute Focal Ischemia", Journal of Neuroscience, 32(22), (May 30, 2012): 7622-31.
  14. Hosmane, Suneil, Million Adane Tegenge, Labchan Rajbhandari, Prech Uapinyoying, Nishant Ganesh Kumar, Nitish Thakor, and Arun Venkatesan, "Toll/Interleukin-1 Receptor Domain-Containing Adapter Inducing Interferon-{β} Mediates Microglial Phagocytosis of Degenerating Axons", Journal of Neuroscience, 32(22), (May 30, 2012): 7745-57.
  15. Barten, Donna M., Patrizia Fanara, Cathy Andorfer, Nina Hoque, P. Y. Anne Wong, Kristofor H. Husted, Gregory W. Cadelina, et al., "Hyperdynamic Microtubules, Cognitive Deficits, and Pathology Are Improved in Tau Transgenic Mice with Low Doses of the Microtubule-Stabilizing Agent BMS-241027", Journal of Neuroscience, 32(21), (May 23, 2012): 7137-45.
  16. Park, Soo Jung, Jee Hoon Lee, Hee Young Kim, Youn Hee Choi, Jung Sup Park, Young Ho Suh, Sang Myun Park, Eun-hye Joe, and Ilo Jou, "Astrocytes, but Not Microglia, Rapidly Sense H2O2 via STAT6 Phosphorylation, Resulting in Cyclooxygenase-2 Expression and Prostaglandin Release", The Journal of Immunology, 188(10), (May 15, 2012): 5132-41.
  17. Ishibashi, Daisuke, Ryuichiro Atarashi, Takayuki Fuse, Takehiro Nakagaki, Naohiro Yamaguchi, Katsuya Satoh, Kenya Honda, and Noriyuki Nishida, "Protective Role of Interferon Regulatory Factor 3-Mediated Signaling Against Prion Infection", Journal of Virology, 86(9), (May 1, 2012): 4947-55.
  18. Ahlenius, Henrik, Karthikeyan Devaraju, Emanuela Monni, Koichi Oki, Somsak Wattananit, Vladimer Darsalia, Robert E. Iosif, et al. "Adaptor Protein LNK Is a Negative Regulator of Brain Neural Stem Cell Proliferation After Stroke", Journal of Neuroscience, 32(15), (April 11, 2012): 5151-64.
  19. Ramirez, Servio H., Janos Hasko, Andrew Skuba, Shongshan Fan, Holly Dykstra, Ryan McCormick, Nancy Reichenbach, et al., "Activation of Cannabinoid Receptor 2 Attenuates Leukocyte-Endothelial Cell Interactions and Blood-Brain Barrier Dysfunction Under Inflammatory Conditions", Journal of Neuroscience, 32(12), (March 21, 2012): 4004-16.
  20. Tanaka, Kohei, Tomoyuki Furuyashiki, Shiho Kitaoka, Yuta Senzai, Yuki Imoto, Eri Segi-Nishida, Yuichi Deguchi, Richard M. Breyer, Matthew D. Breyer, and Shuh Narumiya, "Prostaglandin E2-Mediated Attenuation of Mesocortical Dopaminergic Pathway Is Critical for Susceptibility to Repeated Social Defeat Stress in Mice", Journal of Neuroscience, 32(12), (Mar. 21, 2012): 4319-29.
  21. Haraguchi, Kayo, Ai Kawamoto, Kouichi Isami, Sanae Maeda, Ayaka Kusano, Kayoko Asakura, Hisashi Shirakawa, Yasuo Mori, Takayuki Nakagawa, and Shuji Kaneko, "TRPM2 Contributes to Inflammatory and Neuropathic Pain Through the Aggravation of Pronociceptive Inflammatory Responses in Mice", Journal of Neuroscience, 32, (11), (Mar. 14, 2012): 3931-41.
  22. Fischer, Marie T., Rakhi Sharma, Jamie L. Lim, Lukas Haider, Josa M. Frischer, Joost Drexhage, Don Mahad, Monika Bradl, Jack van Horssen, and Hans Lassmann, "NADPH Oxidase Expression in Active Multiple Sclerosis Lesions in Relation to Oxidative Tissue Damage and Mitochondrial Injury", Brain, 135(3), (Mar. 1, 2012): 886-99.
  23. Font-Nieves, Miriam, M. Gloria Sans-Fons, Roser Gorina, Ester Bonfill-Teixidor, Angelica Salas-Perdomo, Leonardo Marquez-Kisinousky, Tomas Santalucia, and Anna M. Planas, "Induction of COX-2 Enzyme and Down-regulation of COX-1 Expression by Lipopolysaccharide (LPS) Control Prostaglandin E2 Production in Astrocytes", Journal of Biological Chemistry, 287(9), (Febr. 24, 2012): 6454-68.
  24. Antonucci, Flavia, Alan Alpar, Johannes Kacza, Matteo Caleo, Claudia Verderio, Alice Giani, Henrik Martens, et al., "Cracking Down on Inhibition: Selective Removal of GABAergic Interneurons from Hippocampal Networks", Journal of Neuroscience, 32(6), (Feb. 8, 2012): 1989-2001.
  25. Jang, Haeman, David Boltz, Jennifer McClaren, Amar K. Pani, Michelle Smeyne, Ane Korff, Robert Webster, and Richard Jay Smeyne, "Inflammatory Effects of Highly Pathogenic H5N1 Influenza Virus Infection in the CNS of Mice", Journal of Neuroscience, 32(5), (Feb. 1, 2012): 1545-59.
  26. Liu, Shirong, Yang Liu, Wenlin Hao, Lisa Wolf, Amanda J. Kiliaan, Botond Penke, Claudia E. Rube, et al., "TLR2 Is a Primary Receptor for Alzheimer's Amyloid {β} Peptide To Trigger Neuroinflammatory Activation", The Journal of Immunology, 188(3), (February 1, 2012): 1098-1107.
  27. Linnartz, Bettina, Jens Kopatz, Andrea J. Tenner, and Harald Neumann, "Sialic Acid on the Neuronal Glycocalyx Prevents Complement C1 Binding and Complement Receptor-3-Mediated Removal by Microglia", Journal of Neuroscience, 32(3), (Jan. 18, 2012): 946-52.
  28. Roger, Jerome E., Keerthi Ranganath, Lian Zhao, Radu I. Cojocaru, Matthew Brooks, Norimoto Gotoh, Shobi Veleri, et al., "Preservation of Cone Photoreceptors After a Rapid yet Transient Degeneration and Remodeling in Cone-Only Nrl-/- Mouse Retina", Journal of Neuroscience, 32(2), (Jan. 11, 2012): 528-41.
  29. Brown, Karen L., Anton Gossner, Simon Mok, and Neil A. Mabbott, "The Effects of Host Age on the Transport of Complement-Bound Complexes to the Spleen and the Pathogenesis of Intravenous Scrapie Infection", Journal of Virology, 86(1), (Jan. 1, 2012): 25-35.

7. Product List


Product Name Pkg.e Size Wako Cat. No. Grade Storage Condition
Anti Iba1, Rabbit     (for Immunocytochemistry)
50 μg
for Immunochemistry
Keep at -20°C.
Anti Iba1, Rabbit     (for Western blotting)
50 μg

8. Related Products


Product Name Pkg. Size Wako Cat. No. Grade Storage Condition
Anti Iba1, Rabbit, Biotin-conjugated ⇒ details
100 μL
016-26461
for Immunochemistry
Keep at 2 ~ 10°C
Anti Iba1, Rabbit, Red Fluorochrome (635)-conjugated ⇒ details
100 μL
013-26471
Anti Iba1, Monoclonal Antibody (NCNP24) ⇒ details
10 μg
012-26723
Keep at -20°C
50 μg
016-26721
Home > Products > Life Science > ImmunoChemistry  >  Anti Iba1, Rabbit