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For Dementia Research
Anti Tau Monoclonal Antibodies
Total tau / Phospho tau / 3R-tau / 4R-tau / 2N-tau

Tau is a microtubule-associated protein, mainly expressed in neurons in the central nervous system, which regulate stabilization of microtubules. Tau has 6 isoforms by alternative splicing of exon 2, exon 3, exon 10. Alternative splicing of tau exon 10 results in tau isoforms containing either three or four microtubule-binding domains (3R-tau and 4R-tau, respectively). The ratio of 3R-tau/4R-tau is altered in the brains in several tauopathies. Also, the accumulated phosphorylated forms of tau are a major component of neurofibrillary tangles in the brains of Alzheimer’s disease (AD) patients. The degree of these tangles correlates to the severity of dementia. Based on this finding, anti-tau antibodies are ideal for investigation to find out the etiology and to discover drugs for AD. Wako provides 9 kinds of anti tau monoclonal antibodies.

Anti Tau Monoclonal Antibodies


1. LINK

2. Antibody Profile

Reactive tau Antibody profile Clone No.Applications* Cross-reactivity Subtype Epitope Concent- ration
Total tau Recognizes with mouse, rat and human total tau. RTM38 IHC,WB, ICC Mouse Rat HumanRat IgG2a・κ Tau (417-441 a.a.) 1.0-1.2 mg/mL
Total tau (Human specific)Recognizes with specifically human total tau, no-cross reaction to mouse, rat tau. RTM49 IHC,WB, ICCHuman Rat IgG1・κTau (2-44a.a.)1.0-1.2 mg/mL
Total tau (Mouse specific)Recognizes with specifically total tau, no-cross reaction to human, rat tau. RTM47 IHC,WB, ICCMouse Rat IgG2b・κUnknown 1.0-1.2 mg/mL
Phosho-tau T181 Recognizes tau protein phosphorylated at threonine181(T181) 2E2-A6 WB, IHC Human Rat IgG2b・κTau pT181 1.0-1.1 mg/mL
Phosho-tau S199 Recognizes tau protein phosphorylated at Serine 199(S199) 5B8-1E2 WB, IHC Human Rat IgG2a・κTau pS199 1.0-1.1 mg/mL
Phosho-tau S262 Recognizes tau protein phosphorylated at Serine 262(S262) TIP1-35 ELISA Human Rat IgG1・λTau pS262 0.9 mg/mL
2N-tau Recognizes with 2N4R and 2N3R tau isoforms 2C2 WB, IHC Human Rat IgM・κTau (74-103 a.a.)1 mg/mL
3R-tau Recognizes with 0N3R, 1N3R and 2N3R tau isoforms 2A1-1F4 WB, IP Human Rat IgG2b・λ Tau (267-274, 306-313a.a.)1.0-1.1 mg/mL
4R-tau Recognizes with 0N4R, 1N4R and 2N4R tau isoforms 3E8-1A6 WB, IP Human Mouse IgG1・κTau (273-291a.a.)1.0-1.1 mg/mL

3. Application Data

Anti Tau Monoclonal Antibodies■Anti phospho-tau T181 antibody(Clone No. 2E2-A6)
Recognizes tau protein phosphorylated at threonine 181 (phospho-tau T181).

 

Immunohistochemistry Anti Tau Monoclonal Antibodies

→Neurofibrillary tangles containing phospho-tau T181 were immunostained.

Western Blotting Anti Tau Monoclonal Antibodies

→the bands for phospho-tau T181 was scarcely detected in tau mutant T181A.

■Sample
Cell extracts that express the indicated proteins.
(total protein: 25ug)

 

○Human tau WT

 

○Human tau WT + GSK3β

 

○Human tau mutant
T181A+GSK3β

 

○Human tau mutant
S199A + GSK3β

 


Anti Tau Monoclonal Antibodies■Anti phospho-tau S199 antibody(Clone No. 5B8-1E2)
Recognizes tau protein phosphorylated at serine 199 (phospho-tau S199).

 

Immunohistochemistry Anti Tau Monoclonal Antibodies

→Neurofibrillary tangles containing phospho-tau S199 were immunostained.

Western Blotting Anti Tau Monoclonal Antibodies

→the bands for phospho-tau S199 was scarcely detected in tau mutant S199A.

■Sample

Cell extracts that express the indicated proteins. (total protein: 25ug)

(total protein: 25ug)



○Human tau WT

○Human tau WT + GSK3β

○Human tau mutant
T181A+GSK3β



○Human tau mutant
S199A + GSK3β

 


Anti Tau Monoclonal Antibodies■Anti phospho-tau S199 antibody(Clone No. 5B8-1E2)
Recognizes tau protein phosphorylated at serine 199 (phospho-tau S199).

 

 

 

 

Immunohistochemistry Anti Tau Monoclonal Antibodies

→Neurofibrillary tangles containing 2N-tau were immunostained.

Western Blotting Anti Tau Monoclonal Antibodies

→2 bands for 2N-tau were detected.

■Assay Condition

○Sample
Human tau recombinant mixtures(2N4R, 2N3R, 1N4R, 1N3R, 0N4R, 0N3R-tau)



○Load volume
60 ng protein/lane

○Antibody concentration
1/1,000

 


■Anti 3R-tau antibody(Clone No.A1-1F4)

Recognizes with 0N3R, 1N3R and 2N3R tau isoforms.

Anti Tau Monoclonal Antibodies

■Anti 4R-tau antibody(Clone No.3E8-1A6)

Recognizes with 0N4R, 1N4R and 2N4R tau isoforms.

Anti Tau Monoclonal Antibodies

 

○Western Bloting

Anti Tau Monoclonal Antibodies
■Assay Condition

○Sample
Human tau recombinant mixtures
(2N4R, 2N3R, 1N4R, 1N3R, 0N4R, 0N3R-tau)



○Load Volume
60 ng protein/Lane

○Antibody Concentration
 1/2,000


→3 bands for 3R-tau were detected by using anti 3R-tau antibody, and 3 bands for 4R-tau were detected by using the anti 4R-tau antibody.

 

All data were provided by Miyasaka, T., Doshisha University in Japan.

4. Product Lists

Reactive Tau Product Name Wako Cat. No. Pkg. Size Grade Storage Condition
Total tau Anti Human / Mouse / Rat Tau, Rat Monoclonal Antibody (RTM38) 10μL
50μL

011-26891

017-26893

For
Immunochemistry

Keep

at

-20℃

Total tau
(Human specific)
Anti Human Tau, Rat Monoclonal Antibody (RTM49) 10μL
50μL

019-26951

015-26953

Total tau
(Mouse specific)
Anti Mouse Tau, Rat Monoclonal Antibody (RTM47) 10μL
50μL

016-26961

012-26963

Phosho-tau T181 Anti Phosphorylated Tau T181, Rat Monoclonal Antibody (2E2-A6) 10μL
50μL

012-26603

016-26601

Phosho-tau S199Anti Phosphorylated Tau S199, Rat Monoclonal Antibody (5B8-1E2) 10μL
50μL

019-26613

013-26611

Phosho-tau S262Anti Phosphorylated Tau S262, Rat Monoclonal Antibody (TIP1-35) 10μL
50μL

014-27121

010-27123

2N-tauAnti 2N-Tau, Rat Monoclonal Antibody (2C2)10μL
50μL

017-27351

013-27353

3R-tauAnti 3R-Tau, Rat Monoclonal Antibody (2A1-1F4) 10μL
50μL

012-26583

016-26581

4R-tauAnti 4R-Tau, Monoclonal Antibody (3E8-1A6) 10μL
50μL

019-26593

013-26591

 

5. Related Product List

■Tau recombinant

Product Name Wako Cat. No. Pkg. Size Grade Storage Condition
Tau-441 Protein, Human, recombinant

205-20331

100 μgFor
Cell biology

Keep

at

-20℃

Tau-412 Protein, Human, recombinant

208-20321

100 μg
Tau-410 Protein, Human, recombinant

201-20311

100 μg
Tau-383 Protein, Human, recombinant

204-20301

100 μg
Tau-381 Protein, Human, recombinant

201-20291

100 μg
Tau-352 Protein, Human, recombinant

204-20281

100 μg
Tau Protein 4-Repeat Domain, Human, recombinant

209-20351

100 μg
Tau Protein 3-Repeat Domain, Human, recombinant

202-20341

100 μg

 

■Aβ, APP and ApoE antibodies

Product Name Wako Cat. No. Pkg. Size Grade Storage Condition
Anti Human Amyloid β, Monoclonal Antibody (BAN50)

017-26871

013-26873

10μL
50μL
For
Immunochemistry

Keep

at

-20℃

Anti Amyloid β, Monoclonal Antibody (BNT77)

014-26881

010-26883

10μL
50μL
Anti Amyloid β40, Monoclonal Antibody (BA27)

018-26921

014-26923

10μL
50μL
Anti Amyloid β42 (43), Monoclonal Antibody (BC05)

014-26901

010-26903

10μL
50μL
Anti Amyloid Precursor Protein, Monoclonal Antibody (3E9)

014-27241

010-27243

10μL
50μL
Anti Human Apolipoprotein E4, Monoclonal Antibody (1F9)

018-27261

014-27263

10μL
50μL

 

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