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Serum-Free Cell Freezing Medium

BAMBANKER™ is a serum-free medium for long-term freezing at -80ºC and preservation of valuable culture cells such as tumor cells and normal cells.

BAMBANKER™ Direct is improved operability. It doesn't require centrifugation for cell collecting.


User Report
Shota Moriya, Department of Biochemistry, Tokyo Medical University


1. Features

♦ Ready-to-use medium for preservation of cells
♦ Usable without diluting
♦ NO program freezer is required
♦ Rapid and long-term freezing and preservation in a deep freezer (-80ºC)
♦ Serum-free

2. Operating Prodedure


3. Sterility test

Endotoxin:   Chromogenic substrate method
Mycoplasma:   Fluorescent antibody method
Fungi and bacteria:   As per Japanese Pharmacopoeia
**: Certificate of analysis will be issued upon request.


4. Applications

[Cells preserved well by cryopreservation test]
• P3U1 (mouse myeloma cell line), K562 (human leukemia cell line),
  human gastric epithelial cells, human γδT cells
• Daudi (human B cell line), PC12 (rat-derived adrenal pheochromocytoma),
  human B cell line
• OKT4 (mouse hybridoma), monkey B cell line,
  Activated lymphocyte derived from human peripheral blood,
  Activated lymphocyte derived from mouse spleen

5. Comparison of preservation efficiency of mouse fibroblast strain

BAMBANKER™ Self prepared solution
Cells used: Mouse fibroblast cell line
Number of cells stored: 4 × 106 cells / vial. Each cell was suspended in 1mL of preservation solution.
Storage temperature: -80°C. Cells were directly placed in storage box and cooled without using BICELL, etc.
Storage period: 45 days
Thawing method: Cells were thawed according to the conventional method.
Method of measuring
preservatiion efficiency:
After thawing, 5 x 104 cells were inoculated into a 24-well culture plate with 1 mL DMEM medium, and the cell density per culturing area was measured by successive timlapse imaging.
Data by Shota Moriya, Department of Biochemistry, Tokyo Medical University


Better cell viability was obtained using BAMBANKER™ than self-prepared preservative solution.

6. Precautions

♦ Use only for research purposes, not for human use.
♦ Prior to using this medium, perform the confirmation test on the cells under study.
♦ The manufacturer shall not be responsible for any accident or damage caused by the use of this product.
♦ If you have any questions on the use of this medium, please contact the local distributor.

7. Comparison of Bambanker™ with other relevant products

[Cryopreservation Test]



*1: Storage period of Cell Line at -80ºC

Media tested
1. Bambanker™ (LYMPHOTEC Inc.)
2. Medium with serum (Company A)
3. Serum-free Medium (Company A)

8. Product lists

Wako Cat No.
Manufacturer Cat No.
Product Name Package Size Storage Expiration Period
Serum-Free Cell Freezing Medium, containing 10% DMSO
120 mL Keep at
2 ~ 10°C 
2 years
after manufacture 
5 x 20 mL
BAMBANKER™ Direct20 mL
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