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Highly Selective Fluorescent Probe for Hydrogen Peroxide
BES-H2O2-Ac (Cell-permeant)
BES-H2O2 (Cell-impermeant)

Reactive oxygen species (ROS) such as superoxide (O2), hydrogen peroxide (H2O2), and the hydroxyl radical (HO·) are important mediators of pathological processes in various diseases. 2',7'-Dichlorofl uorescein (DCFH) and its diacetyl derivative have been widely used as fluorescent probes for measuring cell-derived H2O2, but these compounds suffer from the major drawback that they are poorly selective toward H2O2. Wako has launched two kinds of BES-H2O2, which are probes for cell-derived H2O2 and cell-impermeat H2O2 with high selectively. BES-H2O2-Ac is applicable to clarifying cell response as well as dynamic function of H2O2 with diseases.

INDEX
  1. Features
  2. Application
  3. References
  4. Products List
  5. Link ⇒ Product Information (664 KB/3P) shown in Wako Product Update Bio-No.1

1. Features

Much higher selectivity compared to conventionally-used DCFH probes
Responds specifically to hydrogen peroxide, enabling detection of intracellular hydrogen peroxide activity
BES-H2O2-Ac is cell membrane permeable
Superior water solubility allows use with aqueous solutions
Can be used in flow cytometry

2. Application
H2O2-produced stimulation No H2O2-produced stimulation
Fluorescent images
Phase-contrast images
Fluorescent images of Jurkat T cells with BES- H2O2 -Ac and the same-fi eld phase-contrast images
Jurkat T cells were cultured in a medium with 50μM BES-H2O2-Ac for 1 hour. Then, one group of them was cultured in a medium with 5 mM butyric acid (H2O2-produced stimulation), whereas the other in a medium without butyric acid (No H2O2- produced stimulation), each for 1 hour. (Courtesy: Professor Hatsuo Maeda, PhD., School of Pharmacy, Hyogo Univ. of Health Sciences)

3. References
  1. "Fluorescent probe for hydrogen peroxide based on a non-oxidative mechanism", Maeda, H. et al.: Angew. Chem. Int. Ed., 43, 2389-91 (2004).
  2. "Hydrogen peroxide-induced deacetylation of acetyl resorufin as a novel indicator reaction for fluorometric detection of glucose using only glucose oxidase", Maeda, H. et al.: Chem. Pharm. Bull., 49, 294-8 (2001).

4. Products List
        < All products are for Cellbiology;   Keep at room temperature >
  Cell Permeability Product Name Package Size Wako Catalog No.
Thiol/Selenol
selective probe
x
BES-Thio
1 mg
025-15481
H2O2 Probes
o
<Detection of
cell-derived H2O2
BES-H2O2-Ac
1 mg
028-17811
x
BES-H2O2   (Cell-impermeant)
1 mg
021-16201
Superoxide
selective probe
o
<Detection of
cell-derived superoxide>
BES-So-AM
1 mg
021-17801
x
BES-So   (Cell-impermeant)
1 mg
028-16211


5. Link
    Link ⇒ Product Information (664 KB/3P) shown in Wako Product Update Bio-No.1 Note


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