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Superoxide Selective Fluorescent Probe
BES-So-AM (Cell-permeant)
BES-So (Cell-impermeant)

Since superoxide (O2·-) is a reactive oxygen having weak cytotoxicity, it is getting attention as a molecule positioned on the uppermost stream side of various reactive oxygen species.O2·- is detected based on different chemiluminescence and fluorescence methods. Among these detection methods, hydroethidine is commonly used but existing probes including hydroethidine are pointed out to have low selectivity for O2·- . BES-So shows a fluorescence by non-redoxreaction dependent mechanism, and indicates high selectivity for O2·-.

INDEX
  1. Features
  2. Application
  3. References
  4. Products List
  5. Link ⇒ Product Information (664 KB/3P) shown in Wako Product Update Bio-No.1



1. Features

Much higher selectivity compared to conventionally-used hydroethidine probes
Responds specifically to superoxide, enabling detection of intracellular superoxide activity
BES-So-AM is cell membrane permeable
Superior water solubility allows use with aqueous solutions
Can be used in flow cytometry

2. Application
(1) O2·--produced stimulation (2) No O2·--produced stimulation (3) O2·--produced stimulation
Fluorescent images
Phase-contrast images
Fluorescent images of Jurkat T cells with BES-So-AM and the same-field phase-contrast images Jurkat T cells were cultured in a medium with 33 μM BES-So-AM at 37℃ for 1 hour. Then, one group (1) was cultured in the medium with 4 mM butyric acid (O2·--produced stimulation), whereas the other group (2) in a medium without butyric acid (no O2·--produced stimulation) at 37℃ for 1 hour. A group (3) was cultured in a medium with 33 μM BES-So-AM and Tiron (super-oxide scavenger), and then 5 mM butyric acid was added in the medium.
(Courtesy: Professor Hatsuo Maeda, Ph.D, School of Pharmacy, Hyogo University of Health Sciences)

3. References
  1. "A design of fluorescent probes for superoxide based on a nonredox mechanism", Maeda, H. et al.: J. Am. Chem. Soc., 127, 68-9 (2005).
  2. "Design of A Practical Fluorescent Probe for Superoxide Based on Protection-Deprotection Chemistry of Fluoresceins with Benzenesulfonyl Protecting Groups", Maeda, H., et al.: Chem. Eur. J., 13, 1946-54 (2007).

4. Products List
        < All products are for Cellbiology;   Keep at room temperature >
 
Cell Permeability
Product Name
Package Size
Wako Catalog No.
Thiol/Selenol
selective probe
x
BES-Thio
1 mg
025-15481
H2O2 Probes
o
<Detection of
cell-derived H2O2
BES-H2O2-Ac
1 mg
028-17811
x
BES-H2O2   (Cell-impermeant)
1 mg
021-16201
Superoxide
selective probe
o
<Detection of
cell-derived superoxide>
BES-So-AM
1 mg
021-17801
x
BES-So   (Cell-impermeant)
1 mg
028-16211

5. Link
    ⇒ Product Information (664 KB/3P) shown in Wako Product Update Bio-No.1

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