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Thiol / Selenol Selective Fluorescent Probe
BES-Thio

Several chemiluminescent and fl uorescent reagents are known as a thiol group detection reagent, and used for detection of thiol groups or measurement of cholinesterase activity. Most of these reagents have a low hydrophilicity, so separate reaction steps, enzyme and detection reactions, are required. BES-Thio has a high hydrophilicity and can be used in aqueous solution. This feature makes it easy to measure the enzyme activity such as cholinesterase using acetylthiocholine or butyrylthiocholine as a substrate. Furthermore, by pH adjustment, BES-Thio can also detect selenol groups, which sulfur (S) in thiol group is substituted by selenium (Se), and can be used for selenoprotein detection reagent.

INDEX
  1. Features
  2. Application
  3. References
  4. Products List
  5. Link ⇒ Product Information (664 KB/3P) shown in Wako Product Update Bio-No.1

1. Features

Selectively Respond to Thiol at pH 7.4
Selectively Respond to Selenol at pH 5.8
Highly water soluble and enables concurrent running of enzyme and detection reactions
* Can also be used for detection of thiol groups such as measurement of cholinesterase activity
2. Application

3. References
  1. "2,4-Dinitrobenzenesulfonyl Fluoresceins as Fluorescent Alternatives to Ellman's Reagent in Thiol-Quantification Enzyme Assays", Maeda, H. et al.: Angew. Chem. Int. Ed., 44, 2922-5 (2005).
  2. "3'-(2,4-Dinitrobenzenesulfonyl)-2',7'-dimethylfluorescein as a Fluorescent Probe for Selenols", Maeda, H. et al .: Angew . Chem. Int. Ed., 45, 1810-3 (2006).

4. Products List
        < All products are for Cellbiology >
  Cell Permeability Product Name Package Size Wako Catalog No.
Thiol/Selenol
selective probe
x
BES-Thio
1 mg
025-15481
H2O2 Probes
o
<Detection of
cell-derived H2O2
BES-H2O2-Ac
1 mg
028-17811
x
BES-H2O2   (Cell-impermeant)
1 mg
021-16201
Superoxide
selective probe
o
<Detection of
cell-derived superoxide>
BES-So-AM
1 mg
021-17801
x
BES-So   (Cell-impermeant)
1 mg
028-16211


5. Link
    ⇒ Product Information (664 KB/3P) shown in Wako Product Update Bio-No.1
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