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Product List for Cell Science Research
⇒ Updated Information: Wako Product Update - Bio-No.1 pdficon 4.97MB
Wako Catalog Number Product Name
1. Antibodies
019-19741 (50μg);
016-20001 (50μg)
Anti Iba-1
013-13781 (1mg/mL) Anti Mouse NGF
016-15731 (200μg) Anti Human NGF
012-18631 (1mL) Anti Rat iNos, Clone #A2
015-18621 (500μL) Anti Rat iNos Peptide
019-18641 (1mL) Anti Rat nNos, Clone# C7
014-15771 (100μg) Anti SNAP-25
018-15791 (100μg) Anti Rat Synaptobrevin
017-15761 (100μg) Anti Rat Synaptotagmin 1
011-15781 (100μg) Anti Rat Syntaxin
019-17801 (20μg) Anti soluble Guanylate Cyclase (sGC),
Clone #mAB3221
017-18201 (20μg) Anti sGC, NO insensitive, Clone #mAB28131
016-13911 (1mL) Anti Substance P (Rabbit)
010-19771
(50μg/100μL)
Anti Rat VGLUT-1, Rabbit
017-19781
(50μg/100μL)
Anti Rat VGLUT-2, Rabbit
2. Apoptosis
039-18241 (100μg) Cytotorienin A, from Streptomyces sp.
291-53204 (24lanes);
297-53201 (96lanes)
Apoptosis Ladder Detection
Kit wako
<Research for Apoptosis>
3. Research for cytomembrane
056-06521(1mg);
052-06523(10mg);
050-06524(100mg)
E-4031 <K+ Channel Blocker>
043-21376(500mg);
043-21371(1g);
047-21374(5g);
041-21372(25g)
Digitonin (water soluble) <Membrane protein
solubilizers>
4. Research for Intracellular Calcium
131-10731 (10μg)
Maitotoxin <Calcium Channel Effector>
193-11831 (100μg)
Stellettamide A
Trifluoroacetate
<Calmodulin inhibitor>
204-08321 (1g)
Trifluoperazine Dimaleate <Calmodulin inhibitor>
5. Research for Cerebral Metabolism
096-05001 (100mg) Idebenone
203-15741 (50mg) T-43362
6. Inhibitors
016-19511 (100μg)
Agosterol A <ABC Transporters specific
inhibitor>
Wako Catalog Number Product Name
042-28661 (100μg)
Dinophysistoxin-I [DTX-I],
from Halichondria okadai
<Protein Phosphatase
inhibitor>
<Related Products>
Okadaic Acid (150-01653 (25μg); 154-01651(100μg); Okadaic Acid Ammonium Salt
(156-02211(100μg); 152-02213(500ug)); Calyculin A (032-14451(100μg);
038-14453(10μg))
164-20153 (10mg);
168-20151 (100mg)
PKSITM-527 <Plasma Kallikrein
Selective inhibitor>
196-12161 (25mg)
Sclerotiorin, from Penicillium
scleotiorum
<Phospholipase A2
inhibitor>
211-01051 (5mg)
U 0126 [1,4-Diamino-2,3-
dicyano-1,4-bis[2-
aminophenylthio]butadiene]
<MAP Kinase Cascade
inhibitor>
<Related Products>
PD-98059 (169-19211; 5mg); SB203580 (190-11581; 1mg)
244-00721 (100μg)
Xestospongin C, from
Xestospongia sp.
<IP3 receptor inhibitor>
<Related Products>
Ryanodine (181-00961; 1mg); Stellettamide A Trifluoroacetate (193-11831; 100μg);
Thapsigargin (549-00301 (1mg); 545-00303 (5mg))
7. Synthetic Retinoids
Am80 (017-16621; 5mg); Am 580 (014-16631; 5mg); Re 80 (180-01391; 5mg);
Ch 55 (039-16781; 5mg); LE 540 (123-04521; 5mg)
<Related products>
Retinoid X Receptor-β, Human, recombinant (187-01421; 50μg); Anti Human
Retinoid X Receptor-β, MAb (012-17031; 100μg); 9-cis-Retinoic Acid (180-01271;
5mg); TTNPB (204-14171; 5mg); all-trans-Retinoic Acid (186-01114 (50mg);
182-01116 (100mg); 182-01111 (250mg); 188-01113 (1g))
8. Cyclicnucleotide related product
011-14441 (10μg)
ADP-ribosyltransferase C3 <Cyclicnucleotide related
product>
9. Neurotoxins
026-07961 (1mg) Bungarotoxin
033-15961 (100mg);
039-15963 (20mg)
Capsaicin
   
   
10. Glutamate Receptors
104-00051 (0.1mg)
Joro Spider Toxin [JSTX-3] <Signal Transduction Agent>
114-00611 (10mg)
Kainic Acid Monohydrate <Signal Transduction Agent>
11. Reagent Kits
299-56701 (50 tests)
Amyloid β-Protein
Immunohistostain Kit
<Research for Alzheimer's
disease>
299-57301 (100 tests)
Phosphorylated Tau
Immunohistostain Kit
<Research for Alzheimer's
disease>
291-53204 (24 lanes);
297-53201 (96 lanes)
Apoptosis Ladder Detection
Kit wako
<Research for Apoptosis>
299-55601 (100 tests)
FluorosparkTM PTP Assay
Kit
<Protein Tyrosine
Phosphatase activity>
12. Mg2+-selective Fluoroionophore
110-00711 (1mg);
116-00713 (5mg)
KMG-20-AM
ALPHABETICAL INDEX
  Description
A ADP-ribosyltransferase C3
Agosterol A
Am 80
Am 580
Amyloid β-Protein Immunohistostain Kit
Anti Iba-1
Anti Mouse NGF
Anti Human NGF
Anti Rat iNOS, MAb
Anti Rat iNOS Peptide
Anti Rat nNOS, MAb
Anti Human Retinoid X Receptor-β, MAb
Anti SNAP-25
Anti Rat Synaptobrevin
Anti Rat Synaptotagmin 1
Anti Rat Syntaxin
Anti soluble Guanylate Cyclase (sGC), MAb
Anti sGC, NO insensitive, MAb
Anti Substance P, Rabbit
Anti VGLUT-1, Rabbit
Anti VGLUT-2, Rabbit
Apoptosis Ladder Detection Kit wako
B Bungarotoxin
C Calyculin A
Capsaicin
Ch 55
Cytotorienin A
D U 0126 [1,4-Diamino-2,3-dicyano-1,4-bis[2-aminophenylthio]
butadiene]
Digitonin (water soluble)
Dinophysistoxin-I [DTX-I]
  Description
E E-4031
F Fluorospark™ PTP Assay Kit -discontinued-
I Idebenone
J Joro Spider Toxin (JSTX-3)
K Kainic Acid Monohydrate
KMG-20-AM
L LE 540
M Maitotoxin
O Okadaic Acid
Okadaic Acid Ammonium Salt
P PD-98059
Phosphorylated Tau Immunohistostain Kit
PKSI™-527
R Re 80
all-trans-Retinoic Acid
9-cis-Retinoic Acid
Retinoid X Receptor-β
Ryanodine
S SB203580
Sclerotiorin
Stellettamide A Trifluoroacetate
T T-43362
Thapsigargin
Trifluperazine Dimaleate
TTNPB
U U 0126 [1,4-Diamino-2,3-dicyano-1,4-bis[2-aminophenylthio]
butadiene]
X Xestospongin C
title_base
Cell Science Research
1. Antibodies
Antibodies against Macrophage/Microglia-specific Protein Iba1
Anti Iba1 polyclonal antibodies, Rabbit   (Iba1: ionized calcium binding adapter molecule 1)
Calcium ions are known to be one of the most important signal mediators in all cells including central nervous system (CNS) cells. Calcium
ions exert their signaling activity through association with various calcium binding proteins, many of which are classified into a large protein
family, the EF hand protein family.
Iba1 is a 17-kDa EF hand protein that is specifically expressed in macrophages/ microglia and is upregulated during the activation of these
cells.
Wako has launched rabbit polyclonal antibodies were raised against a synthetic peptide corresponding to the Iba1 carboxy-terminal sequence,
which was conserved among human, rat and mouse Iba1 protein sequences.     These antibodies are specifically reactive to microglia/
macrophages, are appropriate for immuno-double staining of brain tissues and cell culture in combination with monoclonal antibody to GFAP,
which specifically reacts to astrocyte.
Specificity :
     Specific to microglia and macrophages, but not cross-reactive with neurons and astrocytes.
     Reactive with human, mouse and rat Iba1.
Anti Iba1 polyclonal antibody, Rabbit, for Immunocytochemistry
Wako Cat. #019-19741 50 μg (100 μL)
-20°C, D/I
Working Conc.:Immunocytochemistry 1 - 2μg/mL
Anti Iba1 polyclonal antibody, Rabbit, for Western Blotting
Wako Cat. #016-20001 50 μg (100 μL)
-20°C, D/I
Working Conc.:Westernblot 0.5 - 1 μg/mL
[References]
1) Imai, Y., Ibata, I., Ito, D., Ohsawa, K. and Kohsaka, S.: Biochem. Biophys. Res. Commun., 224, 855 (1996).
2) Ito, D., Imai, Y., Ohsawa, K., Nakajima, K., Fukuuchi, Y. and Kohsaka, S.: Brain Res. Mol. Brain Res., 57, 1 (1998).
3) Ohsawa, K., Imai, Y., Kanazawa, H., Sasaki, Y. and Kohsaka, S.: J. Cell Sci., 113, 3073 (2000).
4) Sasaki, Y., Ohsawa, K., Kanazawa, H., Kohsaka, S. and Imai, Y.: Biochem. Biophys. Res. Commun., 286, 292 (2001).
5) Kanazawa, H., Ohsawa, K., Sasaki, Y., Kohsaka, S. and Imai, Y.: J. Biol. Chem., 277, 20026 (2002).

Figure 1
Immuno-double staining of rat primary mixed culture cells
Green:  Iba1, which reacts to anti Iba1 antibody (Wako C.
          #019-19741)
Red:    astrocyte, which reacts to anti GFAP, monoclonal
          antibody
(Data was provided by Dept. of Neurochemistry, National
Institute of Nueroscience (Japan).)

Anti Rat iNOS, MAb [Clone: A2]
Cat. # 012-18631 for 1mL (2μg/vial)
-20°C, Lyophilized
Lyophilized form in 20 mmol/L HEPES solution (pH 7.3)
containing 0.1% BSA as a stabilizer.
Subclass:IgG1
Specificity:Reactive with iNOS of rat and mouse.
Working Dilution:
Western blot: 1 : 1,000~1 : 3,000
Immunofluorescence 1 : 1
[References]
Dawson, T.M., et al., Meth. Enzymol., 268, 349 (1996).
Anti Rat nNOS, MAb[Clone: C7]
Cat. # 019-18641 for 1mL (40μg/vial)
-20°C, Lyophilized
Lyophilized form in 20 mmol/L HEPES solution (pH 7.3)
containing 0.1% BSA as a stabilizer.
Subclass:IgG1
Specificity:Specific for rat nNOS. Does not react with eNOS and iNOS.
Working Dilution:
Western blot 1 : 500
Immunofluorescence 1 : 10
[References]
1. Bredth, D.S. et al., Neuron, 7, 615 (1991).
2. Lin, C.S. et al., Biochem. Biophys. Res. Commun., 253, 388 (1998).

Anti soluble Guanylate Cyclase (sGC), MAb
[Clone: mAB3221]
Cat. #019-17801 20μg (40μL)
-20°C, D/I, Liquid
Prepared from culture supernatant and prepared in glycine-Tris
solution (pH 7.4). Contains no preservatives and stabilizers.
Isotype:IgG1
Specifically reacts with rat, bovine and human sGC, and strengthens
in the reactivity on activation of sGC by NO, probably, due
to the conformational changes of the enzyme and its associ-ated
antibody-antigen complex.
Working Dilution:
Westernblot 1 : 5,000 ; Immunofluorescence 1 : 250
[References]
Tsuyama, S., et al., FEBS Lett., 455, 291 (1999).
Anti soluble Guanylate Cyclase (sGC), MAb,
NO insensitive[Clone: mAB3221]
Cat. #017-18201 20μg (40μL)
-20°C, D/I, Liquid
Prepared from culture supernatant and prepared in glycine-Tris
solution (pH 7.4). Contains no preservatives and stabilizers.
Isotype:IgG1
Specifically reacts with rat, bovine and human β-subunit of sGC,
but not strengthened in the reactivity on activation of sGC by NO.
Working Dilution:
Westernblot 1 : 5,000 ; Immunofluorescence 1 : 250

Antibodies against Vesicular Glutamate Transporters
Anti Rat VGLUT, Rabbit
L-Glutamate is an excitatory chemical transmitter that plays an essential role in neuronal plasticity, behavior, learning and memory in the
central nervous system. On the other hand, VGLUTs play an essential role in glutamate signal output through vesicular storage of
L-glutamate. Three kinds of VGLUTs have been identified so far. Recent studies have demonstrated that VGLUTs are also expressed in
peripheral cells such as stomach, intestines, pancreas and testes. In particular, the discovery that glutamate is co-localized with glucagon in
secretory granules in_cells of islets of Langerhans has been noted for new mechanism of blood glucose control. Anti Rat VGLUT, Rabbit can
detect glutamatergic central nerves, peripheral nerves and nonneural cells.
Both antibodies are applicable to immunocytochemistry, immunoelectron microscopy and western blotting.
Wako_LOGO
   Raised against the GST fusion peptide encoding G 509 S 560 of the cytosolic regions of VGLUT1
Specificity: Specific to rat, mouse, human and bovine VGLUT-1. No reactive to VGLUT-2
Working Conc.: Immunofluorescence 1 : 1,500
Raised against the GST fusion peptide encoding G 500 Y 582 of cytosolic regions of VGLUT2
Specificity: Specific to rat, mouse, human and bovine VGLUT-2. No reactive to VGLUT-1
Working Conc.: Immunofluorescence 1 : 1,500
Application [1]: Immunofluorescence
Application [2]: Immunoelectron microscopy
Double immunoelectron
microscopy in_cells of islets of
Langerhans.

Glucagon (5nm) and VGLUT2 (15nm)
(arrowheads) are co-localized with
secretory granules.
Photo by Dr. Mitsuko Hayashi
(Yale Univ.)
[References]
1) Moriyama, Y. and Hayashi, M. : TRENDS Pharmacol. Sci.
     electric version, 24, 511 (2003).
2) Morimoto, R., Hayashi, M., Yatsushiro, S., Otsuka, M., Yamamoto,
     A. and Moriyama, Y. : J. Neurochem., 84, 382 (2003).
3) Hayashi, M., Morimoto, R., Yamamoto, A. and Moriyama, Y. :
     J. Histochem. Cytochem., 51, 1375 (2003).
4) Hayashi, M., Yamada, H., Uehara, S., Morimoto, R., Takeda, J.,
     Yamamoto, A, and Moriyama, Y. : J. Biol. Chem., 277, 1966
     (2003).
5) Hayashi, M., Otsuka, M., Morimoto, R., Muroyama, A., Uehara,
     S., Yamamoto, A., and Moriyama Y. : Diabetes, 52, 2066
     (2003).
Wako Catalog No. Description Grade Package Size Storage
010-19771 Anti Rat VGLUT-1,Rabbit for Immunochemistry 50 μg (100 μL) Keep at -20°C
017-19781 Anti Rat VGLUT-2, Rabbit 50 μg (100 μL)
2. Apoptosis
Apoptosis Inducing Bioprobe
Cytotorienin A, from Streptomyces sp.
Cat. #039-18241 100μg
-20°C, D/I, Crystalline powder
A unique bioprobe, cytotrienin A induces apoptosis
(or programmed cell death) in human promyelocytic leukemia HL-
60 cells at a low concentration (10 ng/mL).
Solubility :Soluble in methanol (0.1 mg/mL)
Apoptosis Ladder Detection Kit wako
☞ See Section 11
[References]
1. Kakeya, H., Zhang, H., Kobinata, K. Onose, R., Onozawa, C., Kudo
    T. and Osada, H., "Cytotrienin A, a Novel Apoptosis Inducer in Human
    Leukemia HL-60 Cells", J. Antibiotics, 50(4), 370-372 (1997)
2. Zhang, H., Kakeya, H. and Osada H., "Novel Triene-ansamycins,
    Cytotrienins A and B, Inducing Apoptosis on Human Leukemia HL-
    60 Cells", Tetrahedron Letters, 38(10), 1789-1792 (1997)
3. Kakeya, H., Onose, R., and Osada, H., "Caspase-mediated Activation
    of a 36-kDa Myelin Basic Protein Kinase during Anticancer
    Drug-induced Apoptosis", Cancer Research, 58, 5888-4894 (1998).
4. Watabe, M., Kakeya, H., Onose, R., and Osada, H., "Activation of
    MST/Krs and c-Jun N-terminal Kinases by Different Signaling Pathways
    during Cytotrienin A-induced Apoptosis", J. Biol. Chem., 275,
    8766-8771 (2000).
3. Research for cytomembrane
K+ Channel Blocker
E-4031, 98.0+% (HPLC)
Cat. # 056-06521       1 mg
          052-06523     10 mg
          050-06524   100 mg
RT, Solid
E-4031 selectively suppresses rapid component (Ikr) of the delayed
rectifier potassium current. This makes it a useful tool for
analyzing K+ channel activity in heart cells.
CAS: 113559-13-0
Appearance:Crystalline powder-powder
LD50(mus, intravenous): 112 mg/kg
Applications: [1] Paneling of K+ channel that distributes in the muscle.
                          [2] As a lead compound in investigation to develop
                                antiarrhythmic drugs.
4. Research for Intracellular Calcium
Calmodulin Inhibitors
New Marine Toxin!
Stellettamide A Trifluoroacetate, 95.0+%
Cat. # 193-11831 100µg
20°C, Lyophilized
A marine toxin that is a calmodulin antagonist. It inhibits Ca2+-
calmodulin-dpendent phosphodiesterase.
MW : 514.66 (C26H45N2O CF3COO)
CAS: 129744-24-7
Source: Stelletta sponge
Ref.: Abe, Y. et al., Br. J. Pharmacol., 121, 1309 (1997).
5. Research for Cerebral Metabolism
for Research on Cerebral Metabolism
Idebenone
for Biochemistry
Cat. #096-05001 100 mg
2-10°C, Solid
T-43362
for Biochemistry
Cat. #203-15741 50 mg
2-10°C, Solid
Idebenone is known to act on the central nervous system and ameliorate cerebral apoplexy, cerebral ischemia with affective disorder,
tetraplegia, and impaired passive avoidance response.
T-43362 has a central antioxidant effect with intracerebral aminergic actions. T-43362 exhibits a potent protective effect on glutamate-induced
cells in N18-RE-105 cells and an inhibitory effect on production of lipid peroxide.
[References]    1) p.282, Pharmaceutical Handbook, the 5th edition, edited by Osaka Pharmaceutical Association.
6. Inhibitors
ATP Binding Cassette (ABC) Transporters Specific Inhibitor
Agosterol A
for Biochemistry
Cat. #016-19511 100 µg
-20°C, Solid
Agosterol A is a steroid compound isolated from a marine sponge of Spongia sp. whose habitat is Ago
Bay, Mie prefecture in Japan. Agosterol A was found to specifically inhibit two types of ABC transporters,
P-glycoprotein and multi-drug resistance-associated protein 1. This enabled Agosterol A to inactivate
protein-mediated pump which extrudes chemotherapeutic drugs out of the cell membrane of multi-drug
resistant cancer cells. Agosterol A is a promising substance which completely reverses cancer multi-drug
resistance.
Source : Marine sponge (Spongia sp.)
Appearance : White powder
[References]
1) a) Aoki, S.; Yoshioka, Y.; Miyamoto, Y.; Higuchi, K.; Setiawan, A.; Murakami, N.; Chen, Z-S.; Sumizawa, T.; Akiyama, S.; and Kobayashi, M. Tetrahedron
    Lett.,
1998, 39, 6303-6306.
b) Aoki, S.; Setiawan, A.; Yoshioka, Y.; Higuchi, K.; Fudetani, R.; Chen, Z-S.; Sumizawa, T.; Akiyama, S.; and Kobayashi, M.Tetrahedron, 1999, 55,
    13965-13972.
2) a) Akiyama, S.; Fojo, A.; Hanover, J. A.; Pastan, I.; and Gottesman, M. M. Somat. Cell. Mol. Genet., 1985, 11, 117-126.
    b) Nagayama, S.; Chen, Z-S.; Kitazono, M.; Takebayashi, Y.; Niwa, K.; Yamada, K.; Tani, A.; Haraguchi, M.; Sumizawa, T.; Furukawa, T.; Aikou, T.;
    and Akiyama, S. Cancer Lett. 1998, 130, 175-182.
Dinophysistoxin-1,95.0+% (HPLC)
for Biochemistry
Cat. #042-28661 100 µg
2-10°C, Solid
Dinophysistoxin, isolated from Halichondria okadai, is a diarrhetic shellfish toxin
with 35-methyl okadaic acid. Dinophysistoxin-1 is a potent Non-TPA type tumor
promoter and specifically inhibits protein phosphatases.
TPA : 12-o-Tetradecanoyl-phorbol-13-acetate
[References]  Suganuma, M. et al. : Proc.Natl.Acad.Sci.USA, 85, 1768 (1988).
[Related Products]
Wako Cat. # (Package Size) Description Grade Condition
150-01653 (25µg); 154-01651 (100µg) Okadaic Acid, 80.0+% for Biochemistry 2-10°C, Lyophilized
156-02211 (100µg); 152-02213 (500µg) Okadaic Acid ammonium Salt for Biochemistry 2-10°C, Lyophilized
032-14451 (100µg); 038-14453 (10µg) Calyculin A, 98+ % for Biochemistry -20°C, Solid
Plasma Kallikrein Selective Inhibitor
PKSI™-527, 95.0+% (HPLC)
[N-(trans-4-aminomethylcyclohexenecarbonyl)-L-phenylalanine
4-carboxymethylaniline hydrochloride]
Cat. #164-20153  10mg
         168-20151 100mg
2-10°C, Lyophilized form

in 20 mmol/L HEPES solution (pH 7.3)
Solubility : <10 mmol/L (H2O)
Plasmaprekallikrein circulates in the blood as the zymogen of
plasma kallikrein, and is activated by factor XIIa to form plasma
kallikrein. Plasma kallikrein releases bradykinin from high
molecular weight kininogen. Furthermore, it has been reported
that plasma kallikrein activates factor XII, pro-urokinase and
plasminogen. Plasma kallikrein is also known to cause neutrophil
aggregation and elastase release. These observations suggest that
plasma kallikrein has many function. PKSI-527 inhibited plasma-
kallikrein (PK) with a Ki value of 0.81µm is a great potential tool
in elucidating the significance of PK.
[Kinetic Data]
Enzyme Plasma
kallikrein
Glandular
kallikrein
Plasmin Thrombin Factor Xa Urokinase
Ki (µM) 0.81 >500 390 >500 >500 200
[References]
  Wanaka, K., et al., "Effect of a highly selective plasma-kallikrein
synthetic inhibitor on contact activation relating to kinin generation,
coagulation and fibrinolysis", Thrombosis Research, 57, 889-895
(1990).
Phospholipase A2 Inhibitor
Sclerotiorin, from Penicillium scleotiorum, 97.0+% (HPLC)
Cat. #196-12161       25mg
2-10°C, Solid
A yellow,
chlorine-containing
pigment
Sclerotiorin has
following
activities :
  (1) Phospholipase A2 Inhibitor
  (2) A chlamydospore-like cell-inducing activity (Ref.2)
  (3) Activity in assays for the detection of antagonists of the
        endothelin-A (ETA) and endothelin-B (ETB) receptors (Ref.4)
  (4) A potent inhibitory activity on cholesteryl ester transfer
        protein (CETP) with an IC50 value of 19.4µM. The transfer
        activity of both CE and TG was inhibited to approximately
        the same extent (IC50: 14.4 and 10.3µM, respectively.) (Ref.5)
Solubility : Soluble in chloroform, dichloromethane, acetone
                   and ethyl acetate.

Specific rotation [α]20 (c=1,CHCl3):+450~+550
D
[References]
1. Curtin T.P., et al.: Sclerotiorine, C20H20O5Cl, a chlorine-containing
    metabolic product of Penicillium sclerotiorum van Beyma, Biochem.
    J
., 34, 1419-1421 (1940).
2. Natsume, M., et al.: Chlamydospore-like cell-inducing substances of
    Fungi: close correlation between chemical reactivity with methylamine
    and biological activity, Agric. Biol. Chem., 52(2), 307-312
    (1988).
3. Omura, S., et al.: Isochromophilones I and II, Novel Inhibitors
    against gp120-CD4 binding from Penicillium sp., J. Antibiot., 46(12),
    1908-1911 (1993)
4. Pairet, L., et al.: Azaphilones with Endothelin Receptor binding activity
    produced by Penicillium sclerotiorum: Taxonomy, Fermentation,
    Isolation, structure elucidation and biological activity, J.
    Antibiot
., 48(9), 913-923 (1995).
5. Tomoda, H., et al.: Structure-specific Inhibition of Cholesteryl Ester
    Transfer Protein by Azaphilones, J. Antibiot., 52(2), 160-170 (1999).
MAP Kinase Cascade Inhibitor
U0126, 95.0+%
[1,4-Diamino-2,3-dicyano-1,4-bis [2-aminophenylthio]butadiene]
Cat. #211-01051 5mg
-20°C, D/I, Solid
CAS : 109511-98-2
Solubility : Methanol
A mitogen-activated protein (MAP) kinase cascade inhibitor U0126
inhibits both MEK1 and MEK2 in vitro with comparable IC50 values
(72nM and 58nM, respectively).
[References]
1. Dudley, D, et al., "A synthetic inhibitor of the mitogen-activated pro-
tein kinase cascade", Proc. Nat.. Acad. Sci. USA, 92, 7686-7689
(1995)
2. Alessi, D., et al., "PD098059 Is a Specific Inhibitor of the Activation of
Mitogen-activated Protein Kinase Kinase in Vitro and in Vivo., J. Biol.
Chem
., 270, 27489-27494 (1995)
3. Favata, M., et al., "Identification of a Novel Inhibitor of Mitogen-acti-
vated Protein Kinase Kinase", J. Biol. Chem., 273, 18623-18632
(1998).
4. Mansour, S., et al., "Interdependent Domains Controlling the Enzy-
matic Activity of Mitogen-Activated Protein Kinase Kinase 1", Bio-
chemistry
, 35, 15529-15536 (1996).
[Related Products]
PD-98059   [169-19211, 5mg]
SB203580  [190-11581, 1mg]
Membrane-Permeable Inhibitor of IP3 Receptor
Xestospongin C,from Xestospongia sp., 90+% (HPLC)
Cat. #244-00721 100µg
-20°C, D/I, Lyophilized form in 20 mmol/L HEPES
solution (pH 7.3),packaged under inert gas.
A selective and membrane-permeable inhibitor of the inositol
1,4,5-triphosphate (IP3) receptor-mediated Ca2+ release, isolated
from an Okinawan marine sponge Xestospongia sp. A potent and
highly sensitive inhibitor of IP3 receptor with IC50 of 350nM,
which is 30 times lower than that for ryanodine-receptor.
[References]
Miyamoto, S., et al., "Xestospongin C, a selective and membrance-
permeable inhibitor of IP3 receptor, attenuates the positive inotropic
effect of α-adrenergic stimulation in guinea-pig papillary muscle", Brit-
ish Journal of Pharmacology
, 130, 650-654 (2000)
[Related Products]
Ryanodine   [181-00961, 1mg]
Stellettamide A Trifluoroacetate  [193-11831, 100Ug]
Thapsigargin  [549-00301 (1mg), 545-00303 (5mg)]
7. Synthetic Retinoids
Biologically Active Substances
Retinoids, retinoic acid and its bio-isosters, regulate many biological functions such as cell differentiation, proliferation and embryonic
development in vertebrates, through binding to and activation their specific nuclear receptors. Five representative synthetic retinoids are
available for researcher investigation in signal transduction system through retinoids receptors.
Am 80, 98.0+%(HPLC)
Cat. #017-16621 5mg
RT, Solid
RARα, β-selective agonist
C22H25NO3 = 351.44
Am 580, 98.0+%(HPLC)
Cat. #014-16631 5mg
RT, Solid
RAR-selective agonist
C22H25NO3 = 351.44
Re 80, 95.0+%(HPLC)
Cat. #180-01391 5mg
RT
RAR agonist
C24H26O5 = 3
94.46
Ch 55, 98.0+%(HPLC)
Cat. #039-16781 5mg
RT, Solid
RAR agonist
C24H28O3 = 364.48
LE 540, 98.0+%(HPLC)
Cat. #123-04521 5mg
RT, Solid
RAR agotagonist
C33H32N2O2 = 488.62
[Related Products]
・Retinoid X Receptor-β, Human, recom., Soln. (187-01421, 50µg,
  -70°c, Liquid)
・Anti Human Retinoid X Receptor-β, MAb (#MOK13-17)(012-17031,
  100µg, -20°C, Liquid)(Westernblot 1:400)
・9-cis- Retinoic Acid (180-01271, 5mg, -20°C)
・TTNPB (RAR-selective agonist)(204-14181, 5mg, -20°C)
・all-trans-Retinoic Acid (186-01114 (50mg), 182-01111 (250mg),
  188-01113 (1g), -20°C)
11. Reagent Kits
Amyloid β-Protein Immunohistostain Kit
Cat. #299-56701 50 tests
2-10°C
[Features]
 1. Distinctive histostaining of Aβ40 and 42 plaques in Alzheimer's
     diseased brain tissues
 2. High sensitivity histo-Immune detection of Aβ plaques in tissue
     sections with low background
[Kit Contents (50 tests)]
① Blocking Serum 1 bottle x 10 mL
② Anti Mouse lgG (H+L), Goat, Biotin Conjugated 1 bottle x 10 mL
③ ABC Solution (Streptavidin-biotin-peroxidase Complex) 1 bottle x 10 mL
④ Formic Acid (90%) 1 bottle x 15 mL
⑤ Anti Amyloid β-Protein (1-40), MAb, Clone # BA27 1 bottle x   7 mL
⑥ Anti Amyloid β-Protein (1-42), MAb, Clone # BC05 1 bottle x   7 mL
⑦ Trypsin, Cryst 1 bottle x 50 mg
[Procedures]
  Section of a slide glass plate  
① Deparaffinizing
② Wash in distilled deionized water, for 2 min. x 2
③ Immunopen* treatment Circling section becomes a barrier to protect unnecessary spreading
    of solutions for staining
          *: available from Wako cat. #299-20551
Formic Acid treatment for 5 min. at RT
        ↓
    Wash with a gentle stream of tap water for 5 min. at RT
        ↓
    Rinse with chilled PBS-Tween20 for 5 min. on ice
Trypsin treatment for 15 min. at 37°C
        ↓
    Wash with chilled PBS-Tween20 for 5 min. x 2, on ice
Blocking Serum treatment for 30 min. at 37°C
Primary Antibody (Anti Aβ40 or Aβ42) treatment for 1 hr. at 37°C
        ↓
    Wash with PBS-Tween20 for 2 min. x 5, on ice
Secondary Antibody treatment for 1 hr. at 37°C
        ↓
    Wash with PBS-Tween20 for 2 min. x 3, on ice
ABC treatment for 30 min. at RT
        ↓
    Wash with PBS-Tween20 for 2 min. x 3, on ice
DAB treatment for 2-10 min. at RT
        ↓
Stop the reaction with distilled deionized water for 1 min.
Immunostaining of senile plaques in the consecutive sections of
the brain affected with Alzheimer's Disease.
Left : Aβ42-staining using Anti Aβ42 Ab (Clone #BC05);
Right : Aβ40- staining using Anti Aβ40 Ab (Clone #BA27)
(provided by Dr. Iwatsubo, Univ. of Tokyo)

Phosphorylated Tau Immunohistostain Kit
Cat. #299-57301 100 tests
2-10°C, in the dark
[Features]
 1. A high sensitive histo-immune detection of neurofibrillary lesions in tissue section
    with low background.
 2. Specific staining of hyperphosphorylated tau protein, but not normal tau protein,
    due to use of an antibody to phosphorylated Ser-422 of tau protein
Alzheimer's disease cortex immunostained with mAb
AP422.
Note the staining of numerous neurofibrillary tangles,
neurophil threads and plaque neurites. (provided by
Dr. Hasegawa, Tokyo Institute of Psychiatry)
[Kit Contents (100 tests)]
① Anti Phosphorylated Tau Protein
(pSer-422), Rabbit 1 bottle x 10 mL
② Blocking Serum 1 bottle x 10 mL
③ Anti Rabbit lgG (H+L), Goat, Biotin Conjugated
1 bottle x 10 mL
④ ABC Solution (Streptavidin-biotin-peroxidase Complex)
1 bottle x 10 mL
[Procedures]
  Section of a slide glass plate  
① Deparaffinizing
② Immunopen treatment
③ 3% H2O2 treatment → Wash
④ Blocking
⑤ Primary antibody treatment → Wash
⑥ Secondary antibody Treatment → Wash
⑦ ABC Solution → Wash
⑧ DAB treatment → Stop the reaction

Apoptosis Ladder Detection Kit wako
Cat. # 291-53204      24 lanes
           297-53201      96 lanes
2-10°C
[Features]
  1. High Sensitivity: At least 10³ apoptotic cells can be detected in
     cells and tissues.
  2. Speedy Measurement:  The kit involves about two and half
     hours, from DNA extraction to agarose gel analysis and
     fluorescent staining with SYBR™ GREEN I.
  3. Simple and Highly Reproducible: After mixing with Loading
     Buffer, the recovered DNA can be readily applied to the gel
     slot of the Agarose Gel, provided.
  4. Clear Image of DNA Ladder: DNA is extracted by our own
     unique method, independent of any proteins or lipids contained
     in the cells.
  5. Non-Hazardous: No deleterious solvents, such as phenol and
     chloroform, are used.
[Kit Contents]
① Enzyme Reaction Solution ② RNaset
③ Enzyme Activator ④ Protein Digestion Enzyme
⑤ DNA Extraction Solution ⑥ TE Buffer
⑦ Agarose Gel ⑧ Loading Buffer
⑨ Ladder Marker (123 bp) ⑩ SYBR™ Green I*
*: This reagent is licensed and provided for specific use as a kit
    component by Molecular Probes, Inc., Oregon, USA.

Quenched Fluorescence Substrate Assay of PTP Activity
Fluorospark™ PTP Assay Kit
Cat. #299-55601 100 tests    -sorry, discontinued... -
-20°C, D/I
Phosphorylation and dephosphorylation of protein tyrosine in signal transduction is thought to play a critical role in regulation of physiological
phenomena as immune response, oncogenesis, differentiation, apoptosis, and cell proliferation. Many tyrosine kinases have been cloned and
characterized to understand signaling pathways by phosphorylation, whereas, little is known about the roles of Protein Tyrosine Phosphatase (PTP).
Fluorospark™ PTP Assay Kit consists of all the essential buffers and reagents including quenched fluorescent phosphrylated peptide substrate for
PTP, allowing a homogeneous fluorescent PTP activity assay with a fluorescence microplate reader and a standard fluorometer. The sensitivity by
the standard assay protocol is at 1 pmol or the less, compatible to those of the assay using radio actives.
[Features]
  1. High sensitive measurement of PTP activity at sub-pico moles, compatible to that with RI labeled peptide substrate.
  2. Allowing a homogeneous assay of PTP, which is simple, rapid, and applicable to high throughput screening assay as well as that using
     fluorescence microplate reader.
  3. Allowing the PTP assay even in the presence of phosphate because of indirect measurement of released phosphate.
[Principle]
[Reference]
Nishikata, M., et al., A phosphotyrosine-containing quenched fluorogenic
peptide as a novel substrate for protein tyrosine phosphatases, Biochem. J., 343,
385-391 (1999).
[Kit Contents (100 tests)]
① Anti Substrate Solution (200µmol/L) 110 µL
② Enzyme reaction buffer 1.5 mL
③ 0.2% (w/v) Chymotrypsin solution 220 µL
④ Calibrator
  (containing MCA-Gly-Asp-Gly-Val-Tyr) 40 µL
⑤ Stop solution (10 mmol/L sodium vanadate) 220 µL
Kinetic experiment
Endpoint experiment
Measurement of PTP activity in cytoplasmic
fraction of osteoblast like cell line (Data was
provided by Dept. of Dental Pharmacology,
Hokkaido Univ. School of Dentistry (Japan))
12. Mg2+-selective Fluoroionophore
KMG-20-AM
Cat. #110-00711   1 mg
        #116-00713   5 mg
-20°C, D/I, Solid
Dynamic distribution of Mg2+ in living cells can be done due to
selective recognition of Mg2+ by KMG-20-AM. KMG-20-AM is
much less reactive to Ca2+ than Mg2+.

KMG-20-AM enables accurate measurement of Mg2+ because it has
very much low affinity to Ca2+ compared to Mg2+.
Appearance: Brown, powder
Assay (HPLC): 95+ %
[Features]
 1. Mg2+-imaging without interference of Ca2+
 2. Precise observation of Mg22+ distribution by Fluorescent
     Microscopy
 3. Direct observation of Mg2+ ion dynamics in living cells
[Fluorescent imaging]
[Reference]
1) Nagashima, H., Tohda, K., Matsunari, Y.,Tsunakwa, Y., Watanabe, K.,
     Inoue, H. and Suzuki, K.: Anal. Lett., 23, 1993(1990).
2) Suzuki, K., Watanabe, K., Matsumoto, Y.,Kobayashi, M., Sato, S., Siswanta,
     D. and Hisamoto, H.: Anal. Chem., 67, 324(1995).
3) Suzuki, Y., Saito, N., Komatsu, H., Citterio,D., Kitamura, Y., Kubota, T., Oka,
     K. and Suzuki, K.: Anal. Sci., 17, i1451(2001).
4) Suzuki, Y., Komatsu, H., Ikeda, T., Saito, N.,Araki, S., Citterio, D., Hisamoto,
     H., Kitamura,Y., Kubota, T., Nakagawa, J., Oka, K. and Suzuki, K.: Anal.
     Chem., 74, 1423(2002).
5) Haugland, R. P.: "Handbook of Fluorescent Probes and Research Products,
     7th ed. ",Molecular Probes Inc.
6) Kubota, T., Tokuno, K., Nakagawa, J.,Kitamura, Y., Ogawa, H., Suzuki, Y.,
     Suzuki,K. and Oka, K.: Biochem. Biophys. Res.Commun., 303, 332(2003).