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Surfactant for addition of Insect Cell Culture Medium
CultureSure
10w/v% Polyoxyethylene Polyoxypropylene Glycol Solution

This product is a 10% aqueous solution of non-ionic surfactants for cell culture.
In the insect cell culture, it is added to the culture medium so as to prevent adhesion to the flask or inhibit the aggregation.
In addition, it has been reported that its action as defoaming agent protects the cells from damage due to contact with air bubbles, and by the preventing action of shear fracture due to aeration stirring, it is effective in cell proliferation.


1. Features

  • Ready-to-use solution
  • Endotoxin: max.0.5 EU/mL
  • Sterility tested
  • Mycoplasma tested

CultureSure

Molecular formula: OH(C2H4O)x・(C3H6O)y・(C2H4O)zH
CAS: 9003-11-6

2. References

  1. David W. M. and Charles F. G.: BIO/TECHNOLOGY, 6, 1411(1988).
  2. Jordan M. et al: Biotechnol Bioeng. , 43, 446(1994).
  3. Gilbert R. S. et al: Cytotechnology, 22, 211(1996).

3. Product List

Product Name Package Size Wako Catalog No. Grade Storage Condition
CultureSure 10 w/v% Polyoxyethylene Polyoxypropylene Glycol Solution 100 mL 033-23391 for Cell Culture Keep at 2-10°C.

4. Related Products

4-1. Liquid medium for insect culture

Product Name Package Size Wako Catalog No. Grade Storage Condition
PSFM-J1 Medium Wako, Liquid 1 L 160-25851 for Cell Culture Keep at 2-10°C.

4-2. Reagent for adding medium

Product Name Package Size Wako Catalog No. Grade Storage Condition
Cholesterol, Semi-synthetic 5 g 034-22961 for Cell Culture Keep at 2-10°C.
25 g 032-22962
Cod Liver Oil 250 g 030-23041 for Biochemistry Keep at RT.
Polyoxyethylene Polyoxypropylene Glycol (160E.O.) (30P.O.) 100 g 160-25231
500 g 162-25235
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