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For a research on inositol phospholipid signaling
Anti Human Diacylglycerol Kinaseδ, Rabbit


Diacylglycerol kinase (DGK) is an enzyme that regulates intracellular concentration of diacylglycerol(DG) and phosphatidic acid (PA) in the inositol phospholipid signaling. DG activates PKC as a second messenger of the signal generated by phospholipase C that is produced by receptor stimulation. Currently, ten subtypes of the DGK have been reported in mammals. It is reported that δ subtype (molecular weight 130,000) is abundantly expressed in muscles and regulates the activity of EGF receptors and insulin receptors.
This time Wako has released two products, antiserum of human DGKδ for the Wester blotting and antibody of human DGKδ for immunoprecipitation.





1. Product Outline



for Blotting

Package Insert 019-22791(WB) (84.4 KB/1 page)

  • Preparation: Rabbit antiserum, prepared in 50% glycerol solution containing 0.02% sodium azide.
  • Antigen: bacterially expressed fragmant corresponding to C-terminals of δ1 or δ2 of human DGKδ
  • Purification: None
  • Specificity: Specific to human DGKδ (others not tested)
  • Working Dilution: Western blot 1 : 1,000 - 1 : 2,000

for Immunoprecipitation

Package Insert 012-22801(IP) (86.2 KB/1 page)

  • Preparation: PBS solution with 50% glycerol
  • Antigen: synthetic peptide corresponding to C-terminals of δ1 or δ2 of human DGKδ.
  • Purification: Purified by protein A affinity chromatography
  • Specificity: Specific to human DGKδ
  • Concentration: Indicated on each label (first lot: 5 mg/mL)
  • Working Dilution: Immunoprecipitation 5 - 10 μg/test




2. Example of use


DGKd

After immunoprecipitation was conducted in HEK293 cell lysate (Input) with the anti-DGKδ antibody for immunoprecipitation (Wako Cat. No.012-22801)(IP), Western blot was conducted with the anti DGKδ antibody for blotting (Wako Cat. No. 019-22791)(WB). Rabbit IgG was used as a negative control (N). Intracellular DGKδ2 was detected by immunoprecipitation (δ).

<Data was provided by: Dr. Fumio Sakane, Laboratory of Biofunctional Chemistry, Graduate School of Science, Chiba University>


3. References



  1. Sakane, F., Imai, S., Kai, M., Wada, I. and Kanoh, H. : J. Biol. Chem., 271, 8394 (1996)
  2. Imai, S., Yasuda, S., Kai, M., Kanoh, H. and Sakane, F. : Biochim. Biophys. Acta, 1791, 246 (2009)

4. Product List


Product Name Package Size Wako Catalog No. Grade Storage Condition
Anti Human Diacylglycerol Kinaseδ, Rabbit
50 μL
for Blotting
Keep at -20°C.
50 μL
for Immunoprecipitation


5. Related Product


Product Name Package Size Wako Catalog No. Grade Storage Condition
Anti Rat Diacylglycerol Kinaseγ, Rabbit
50 μL
for Immunochemistry
Keep at -80°C.


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