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For a research on inositol phospholipid signaling
Anti Rat Diacylglycerol Kinaseγ, Rabbit


Diacylglycerol kinase (DGK) is an enzyme that regulates intracellular concentration of diacylglycerol (DG) and phosphatidic acid (PA) in the inositol phospholipid signaling. DG affects PKCγ and activates it as a second messenger of a signal generated by phospholipase C that is produced by receptor stimulation. Currently, ten subtypes of the DGK have been reported in mammals and it is reported that γ subtypes (molecular weight 88,000) are abundantly expressed in neurons such as Purkinje cells and directly interact with γPKC or inhibit the activity of the small G-protein Rac1 (concern with cytoskeleton, regulation of cell morphology, and cancer invasion) that is increased by EGF stimulation.





1. Product Outline


  • Form: Antiserum (contains no preservatives or stabilizers)
  • Antigen: A GST-fusion protein that corresponds to the sequence close to the N-terminal of rat DGKγ
  • Purification: No
  • Specificity: Cross-react with rat or mouse DGKγ (not tested as for the other)
  • Concentration of use: Western blotting 1:1,000 to1:8,000
    Immunoprecipitation 2 to 20 μl/Test
    Immunohistochemistry 1:10,000 to1:15,000
    Immunofluorescent staining 1:5,000


2. Example of use

DGKgamma

<Fluorescent staining of rat Purkinje cells>

Fix: 4% paraformaldehyde, 0.1M phosphate buffer solution containing 0.2% picric acid (pH 7.4)
Blocking: Incubate in 0.01M PBS containing 0.3%Triton X-100 for 20 minutes followed by addition of
10% normal goat serum and incubate for 20 minutes
Primary antibody: This product (1:5,000), 4°C, 16 hours
Secondary antibody: Alexa 586-labeled goat anti-rabbit IgG (1:2,000), 1 hour

<Data was provided by Dr. Naoaki Saito, Dr. Yasuhito Shirai, Biosignal Research Center, Kobe University)


3. References


  1. Sakane, F., Imai, S., Kai, M., Wada, I. and Kanoh, H. : J. Biol. Chem., 271, 8394 (1996)
  2. Imai, S., Yasuda, S., Kai, M., Kanoh, H. and Sakane, F. : Biochim. Biophys. Acta, 1791, 246 (2009)


4. Product List

Product Name Package Size Wako Catalog No. Grade Storage Condition
Anti Rat Diacylglycerol Kinaseγ, Rabbit
50 μL
for Immunochemistry
Keep at -80°C.


5. Related Product

Product Name Package Size Wako Catalog No. Grade Storage Condition
Anti Human Diacylglycerol Kinaseδ, Rabbit
50 μL
for Blotting
Keep at -20°C.
50 μL
for Immunoprecipitation


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