Wako | DNA Extractor SP Kit

Features

DNA Extractor SP Kit efficiently extracts DNA fragments contained in serum and plasma. Based on the Sodium Iodide (NaI) method¹, this kit enables the whole procedures done in a single microcentrifuge tube without using hazardous phenol/chloroform. Recently, tumor-specific genes have been amplified and detected in the serum and plasma of patients with various diseasessuch as lung, breast and colon cancer. Many of these reference articles are currently being published. The kit is a powerful pretreatment reagent for the detection and analysis of target DNA because of its high quality and yield.^2,3,4
	High DNA yield (about 100 %) from small amount of serum and plasma
	The DNA extraction procedure is based on a simple centrifugal separation using Sodium Iodide and alcohol, that results in less loss of DNA.
	Safe operation
	No phenol or chloroform required
	Minimum contamination
	The entire extraction of DNA can be done in a single centrifuge tube.
	Complete removal of lipid derived from blood
	A specialized alcohol solution in original formula
	Less variability in extraction from sample to sample

Product Figure

Kit Contents: 50 reactions

• Enzyme reaction solution 1 X 10 mL	• Alcohol solution 1 X 30 mL
• Protein digestion solution 1 X 250 µL	• Washing Solution (A) 1 X 50 mL
• Sodium iodide solution 1 X 15 mL	• Washing Solution (B) 1 X 50 mL

Application data 1:

Result of amplification of p53-Exon 5 region extracted from human serum and plasma
By using DNA Extractor SP Kit, DNA was extracted from 10 human serum and one plasma samples. The extracted DNA was finally resolved in 20 µL of TE (pH 8.0), and 5 µL of that was subject to amplification of p53-Exon 5 (308 bp). Commercial available kit based on a glass binding method (spin column method) was used as a comparative method.

Application data 2:

Result of amplification of p53-Exon 7 region extracted from human serum
DNA was extracted from Serum 2 and 3 used in the test data 1 and another human serum (Serum 11) for amplification of p53-Exon 7 (439 bp).

References

1) Ishizawa, M., Kobayashi, Y., Miyamura, T. and Matsuura, S.: Nucleic Acids Res., 22, 1774 (1994).
2) Sozzi, G., Musso, K., Ratcliffe, C., Goldstraw, P., Pierotti, M. A. and Pastorino, U.: Clin Cancer Res., 5, 2689 (1999).
3) Silva, J. M., Dominguez, G., Garcia, J. M., Gonzalez, R., Villanueva, M. J., Navarro, F., Provencio, M., San, Martin, S., Espana, P. and Bonilla, F.: Cancer Res., 59, 3251 (1999)
4) Shao, Z. M., Wu, J., Shen, Z. Z. and Nguyen, M.: Clin Cancer Res., 7, 2222 (2001).

Storage condition

Keep at 2~10 ºC

Product List

Product name	Usable samples	Catalog No.	Package size
DNA Extractor Kit	Plasma, serum	296-60501	50 tests