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for Maintenance of Differentiation Potential and Induced Differentiation of ES/iPS Cells
Reagents for ES/iPS Cell Research

   ⇒ Leaflet (531 KB/ 6 pages)

Since the announcement of induction of first iPS cells in 2007, a lot of literature has been published on iPS cells. These low-molecular compounds have been frequently reported in literature tinvolved in maintenance of the differentiation potential and induction of differentiation of embryonic stem cells and iPS cells.

We now have a solution type of Y-27632 which has been popular in human iPS cell culture. The price for well-received CHIR99021 has been lowered.



INDEX

  1. Featured Products
    ·(-)-Blebbistatin
    ·CHIR99021
    ·PD0325901
    ·PD184352
    ·SB431542
    ·SU5402
    ·Thiazovivin
    ·Y-27632

  2. Product List
    [ 2-a ]   Maintenance of differentiation potential and enhancement of reprogramming efficiency of ES/iPS cells
    · A-83-01
    · ACTH
    · ALK5 Inhibitor
    · (±)-BayK8644
    · BIX01294
    · (-)-Blebbistatin
    · BIO
    · Butyric Acid
    NEW! · CHIR99021
    · Cyclic Pifithrin-α Hydrobromide
    · RG108
    · EHNA Hydrochloride
    · GSK269962A
    · GSK429286A
    · H1152 Dihydrochloride
    · IQ-1
    · Kenpaullone
    · PD0325901
    · PD173074
    · PD184352
    · PD-98059
    · PS48
    NEW! · SB203580
    · SB203580 Hydrochloride
    · SC-1
    · Sodium Butyrate
    · SR3677 Hydrochloride
    · SU5402
    · Thiazovivin
    · Valproic Acid
    · Y-27632
    NEW! · Y-27632 Solution

    [ 2-b ]    Differentiation induction and dedifferentiation of ES/iPS cells

    · A769662
    · AICAR
    · Ciclosporin A
    · Ciglitazone
    · CKI-7 Hydrochloride
    · DAPT
    · Dexamethasone
    · Dorsomorphin
    · Dorsomorphin Dihydrochloride
    · Indolactam V
    · IPA-3
    · IWP-2
    · IWR-1-endo
    · LDN193189 Hydrochloride
    · LY-294002
    · Purmorphamine
    · all-trans-Retinoic Acid
    · Reversine
    NEW! SB431542
    · Spermine
    · Trichostatin A
    · Troglitazone
    · TWS119

    [ 2-c ]    StemSure Series
    [ 2-d ]    LIF, human recombinant, Culture Supernatant
    [ 2-e ]    N2 Supplement, with Transferrin (Holo)/ (Apo)
    [ 2-f ]    Products for Cell Culture
    [ 2-g ]    BAMBANKER, Serum-Free Cell Freezing Medium for ES/iPS Cell Research
    [ 2-h ]    Sumitomo Bakelite's Product Line  for Regenerative Medicine Research


1. Featured Products

· (-)-Blebbistatin

A myosin II-specific inhibitor. Inhibits blebbing in human embryonic stem cells and human iPS cells dissociated from the colony and prevents apoptosis.

Assay: 98.0+ % (HPLC)
Solubility: DMSO(5 mg/ mL)
CAS No.: 56925-71-8
C18H16N2O2= 292.33

Reference: Ohgushi, M., et al.: Cell Stem Cell, 7, 225, (2010).

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· CHIR99021

A highly-selective GSK-3 inhibitor. Use of this product with PD184352,SU5402, or PD0325901 enables efficient culture of murine embryonic stem cells while maintaining the differentiation potential.

Assay: 95.0+ % (HPLC)
Solubility: DMSO(0.5 mg/ mL verified)
CAS No.: 52917-06-9
C22H18Cl2N8 = 465.34

Reference: Ying, QL., et al.: Nature, 453, 519 (2008).


· PD0325901

An MAPK inhibitor. Murine embryonic stem cells can be cultured efficiently when this product is used with CHIR99021. Use with SB431542 and thiazovivin enhances the efficiency of reprogramming to human iPS cells by over 200 times and speeds up reprogramming.

Assay: 98.0+ % (HPLC)
Solubility: DMSO (approx. 20 mg/mL)
CAS No.: 91210-10-9
C16H14F3IN2O4 = 482.19

Reference:
·   Ying, QL., et al.: Nature, 453, 519 (2008).
·   Lin, T., et al.: Nat. Methods, 6, 805 (2009).


· PD184352

A highly-selective MEK1 inhibitor. Use with CHIR99021 and SU5402 enables efficient culture of murine embryonic stem cells while keeping the differentiation potential.

Assay: 98.0+ % (HPLC)
Solubility: DMSO(30 mg/ mL)
CAS No.: 12631-79-3
C17H14ClF2IN2O2 = 478.66

Reference: Ying, QL., et al.: Nature, 453, 519 (2008).

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· SB431542

This is an ALK4/ALK5/ALK7 inhibitor. Stimulates proliferation, differentiation, and sheet formation of endothelial cells derived from embryonic stem cells. Use with thiazovivin and PD0325901 enhances reprogramming efficiency by over 200-fold and increases reprogramming speed.
Assay: 98.0+ % (HPLC)
Solubility: DMSO(10 mg/ mL)
CAS No.: 01836-41-9
C22H16IN4O3 = 384.39

Reference:
·  Ogawa, K., et al.: J. Cell. Sci., 120, 55 (2007).
·  Lin, T., et al.: Nat. Methods, 6, 805, (2009).

· SU5402

An FGFR1 inhibitor. Use with CHIR99021 and PD184352 enables efficient culture of murine embryonic stem cells while maintaining the differentiation potential.
Assay: 95.0+ % (HPLC)
Solubility: DMSO(1 mg/ mL)
CAS No.: 215543-92-3
C17H16N2O3 = 296.32

Reference: Ying, QL., et al.: Nature, 453, 519 (2008).

· Thiazovivin

Use with SB431542 and PD0325901 has been reported to enhance reprogramming efficiency by over 200-fold and increase reprogramming speed.
Assay: 95.0+ % (HPLC)
Solubility: DMSO (1 mg/ mL)
CAS No.: 1226056-71-8
C15H13N5OS = 311.36

Reference: Lin, T., et al.: Nat. Methods, 6, 805, (2009).




· Y-27632

A ROCK inhibitor. Increases the post-cryopreservation survival and cloning efficiency of human embryonic stem cells and human iPS cells.
Assay: 98.0+ % (HPLC)
Solubility: Water (2.5 mg/mL: confirmed)
CAS No.: 331752-47-7
C14H21N3O·2HCl·H2O = 338.27

References:
  1. Kawamata, M., et al.: Proc. Natl. Acad. Sci. USA., 107, 14223 (2010).

  2. Claassen, DA., et al.: Mol. Reprod. Dev., 76, 722(2009).
  3. Martin-Ibanez, R., et al.: Hum. Reprod., 23, 2744(2008).
  4. Watanabe, K., et al.: Nat. Biotechnol., 25, 681(2007).
  5. Sakamoto, K., et al.: J. Pharamacol. Sci., 92, 56(2003).
  6. Nishimaru, K., et al.: J. Pharamacol. Sci., 92, 424(2003).
  7. Uehata, M., et al.: Nature, 389, 990 (1997).
*This is marketed under the license of Mitsubishi Tanabe Pharma Corporation.
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2. Product List
[ 2-a ]    Maintenance ES & iPS cells in an undifferentiated state & Improve reprogramming efficiency    ⇒ Leaflet
Product Name Grade Pkg. Size Wako Cat. #
A-83-01 【TGF-β RI Kinase Inhibitor IV】 for Cellbiology 2 mg
10 mg
018-22521
014-22523
An ALK4/ALK5/ALK7 inhibitor. Is useful for long-term homogeneous culture of rat and dog iPS cells without differentiation.
〔Li, W., et al.: Cell Stem Cell, 4, 16 (2009).]
Adrenocorticotropic Hormone(1-24)(Human) 【ACTH】 for Cellbiology 1 mg 013-22211
Inhibits differentiation of murine embryonic stem cells and enables proliferation of embryonic stem cells in serum-free culture medium.
〔Ogawa, K., et al.: Genes Cells, 9, 471(2004).]
ALK5 Inhibitor 【TGF-β RI Kinase Inhibitor Ⅱ】 for Cellbiology 1 mg 012-23021
A TGF-β RI kinase/ALK5 inhibitor. Can be used as substitute of Sox2 or c-Myc (Yamanaka 4 factors) in induction of murine iPS cells.
〔Maherali, N., et al.: Curr. Biol., 19, 1718 (2009).]
(±)BayK8644 for Biochemistry 5 mg 027-09951
An L-type Ca2+ channel agonist. Use with BIX01294 when Oct3/4 and Klf4 are delivered to fibroblasts can enhance the efficiency of iPS cell induction.
〔Shi, Y., et al.: Cell Stem Cell, 3, 568 (2008).]
BIX01294 for Cellbiology 2 mg 023-16401
A histone methyltransferase inhibitor. Enhances the efficiency of iPS cell induction when used during delivery of Oct3/4 and Klf4 to neural stem cells.   Use of this product with (±)BayK8644 during delivery of Oct3/4 and Klf4 to fibroblasts also increases the efficiency of iPS cell induction.
〔Shi, Y., et al.: Cell Stem Cell, 2, 525 (2008).〕
〔Shi, Y., et al.: Cell Stem Cell, 3, 568 (2008).]
(-)-Blebbistatin for Cellbiology 1 mg
5 mg
021-17041
027-17043
A histone methyltransferase inhibitor. Enhances the efficiency of iPS cell induction when used during delivery of Oct3/4 and Klf4 to neural stem cells. Use of this product with (±)BayK8644 during delivery of Oct3/4 and Klf4 to fibroblasts also increases the efficiency of iPS cell induction.
〔Ohgushi, M., et al.: Cell Stem Cell, 7, 225, (2010).]
6-Bromoindirubin-3'-oxime 【BIO】【GSK-3 Inhibitor IX】 for Cellbiology 1 mg 029-16241
A GSK-3 inhibitor. Maintains the differentiation potential of human and murine embryonic stem cells in feeder cell-free culture medium.
〔Sato, N., et al.: Nat. Med., 10, 55 (2004).]
Butyric Acid Wako Special Grade 25ml
500ml
029-05393
023-05396
Improves iPS cell induction efficiency.
〔Liang, G., et al.: J. Biol. Chem., 285, 25516 (2010).]
NEW! CHIR99021 【CT99021】 for Cellbiology 1 mg
5 mg
032-22401
038-22403
Highly-selective GSK-3 inhibitor. Use with PD184352, SU5402, or PD0325901 enables efficient culture of embryonic stem cells while keeping the differentiation potential.
〔Ying, QL., et al.: Nature, 453, 519 (2008).]
Cyclic Pifithrin-α Hydrobromide for Cellbiology 5 mg 035-22131
A reversible inhibitor of p53-mediated apoptosis and p53-dependent gene transcription. A decrease in p53 activity enhances the reprogramming efficiency of human and mouse somatic cells.
DNA Methyltransferase Inhibitor 【RG108】 For gene research 10 mg
25 mg
041-30101
047-30103
A DNA methyltransferase inhibitor. Inhibits cell proliferation with low cytotoxicity.
EHNA Hydrochloride for Cellbiology 10 mg 056-08221
A phosphodiesterase/adenosine deaminase inhibitor. Can be used as substitute of bFGF. Inhibits human embryonic stem cell differentiation efficiently and reversibly without feeder cells and maintains pluripotency.
〔Burton, P., et al.: Biochem. J., 575, 432, (2010).]
GSK269962A for Cellbiology 2 mg 070-05921
A ROCK inhibitor. ROCK inhibitors reportedly inhibit apoptosis and improve the survival and cloning efficiency of dissociated human embryonic stem cells.
GSK429286A for Cellbiology 2 mg 073-05911
A ROCK inhibitor. ROCK inhibitors reportedly inhibit apoptosis and improve the survival and cloning efficiency of dissociated human embryonic stem cells.
H1152 Dihydrochloride for Cellbiology 1 mg 088-09281
A ROCK inhibitor. ROCK inhibitors reportedly inhibit apoptosis and improve the survival and cloning efficiency of dissociated human embryonic stem cells.Has a stronger inhibitory effect than Y-27632.
IQ-1 for Cellbiology 5 mg 095-05951
Binds to Wnt-3a and maintains proliferation and pluripotency of murine embryonic stem cells without serum.
〔Miyabayashi, T., et al.: Proc. Natl. Acad. Sci. USA., 104, 8912 (2009).]
Kenpaullone for Cellbiology 1 mg
5 mg
110-00831
116-00833
An inhibitor of GSK-3 and CDKs. Can be used as substitute of Klf4 (one of Yamanaka 4 factors) during iPS cell induction. Adding it during delivery of Yamanaka 4 factors will also enhance reprogramming efficiency.
〔Lyssiotis, CA., et al.: Proc. Natl. Acad. Sci. USA., 106, 8912 (2009).]
PD0325901 for Cellbiology 5 mg
25 mg
162-25291
168-25293
An MAPK inhibitor. Use with CHIR99021 enables efficient culture of embryonic stem cells while maintaining the differentiation potential. Use with SB431542 and thiazovivin also enhances reprogramming efficiency to human iPS cells by over 200-fold and increases reprogramming speed.
〔Ying, QL., et al.: Nature, 453, 519 (2008).]
〔Lin, T., et al.: Nat. Methods, 6, 805, (2009).]
PD173074 for Cellbiology 5 mg 166-24351
An FGFR inhibitor. Stimulates self-proliferation of murine embryonic stem cells.
〔Ying, QL., et al.: Nature, 453, 519 (2008).]
PD184352 for Cellbiology 5 mg 169-25181
An MEK1 inhibitor. Use with CHIR99021 and SU5402 enables efficient culture of murine embryonic stem cells while maintaining the differentiation potential.
〔Ying, QL., et al.: Nature, 453, 519 (2008).]
PD-98059 for Biochemistry 5 mg 169-19211
An MAPK inhibitor. Promotes self-proliferation of embryonic stem cells.
〔Burdon, T., et al.: Dev. Biol., 210, 30 (1999).]
〔Hamazaki, T., et al.: Mol. Cell Biol., 26, 7539 (2006).]
PS48 for Cellbiology 5 mg 164-25011
A PDK1 inhibitor. Use with Oct4 and Klf4 during induction of iPS cells from neonatal human epithelial keratinocytes (NHEKs) increases reprogramming efficiency by 15-fold. Adding it with sodium butyrate enhances induction efficiency by 25-fold.
〔Zhu, S., et al.: Cell Stem Cell, 7, 651 (2010).]
NEW! SB203580 for Cellbiology 1 mg
5 mg
199-16551
195-16553
An MAPK inhibitor. Used for isolation and maintenance of embryonic stem cells.
〔Qi, X., et al.: Proc. Natl. Acad. Sci. USA., 101, 6027 (2004).]
SB203580 Hydrochloride for Cellbiology 1 mg 193-15611
An MAPK inhibitor. Used for isolation and maintenance of embryonic stem cells.
〔Qi, X., et al.: Proc. Natl. Acad. Sci. USA., 101, 6027 (2004).]
SC-1 【Pluripotin】 for Cellbiology 1 mg 191-15411
A RasGAP and ERK1 inhibitor. Maintains the differentiation potential of murine embryonic stem cells in LIF-free, feeder cell-free, and serum-free culture medium.
〔Chen, S., et al.: Proc. Natl. Acad. Sci. USA., 103, 17266 (2006).]
Sodium Butyrate - 25 g
500 g
193-01522
197-01525
A histone deacetylase inhibitor. Promotes self-proliferation of embryonic stem cells.
〔Ware, CB., et al.: Cell Stem Cell, 4, 359 (2009).]
SR3677 Hydrochloride for Cellbiology 2 mg 195-16151
A ROCK inhibitor. ROCK inhibitors reportedly inhibit apoptosis and improve the survival and cloning efficiency of dissociated human embryonic stem cells.
SU5402 for Cellbiology 1 mg 193-16071
An FGFR1 inhibitor. Use with CHIR99021 and PD184352 enables efficient culture of murine embryonic stem cells while maintaining the differentiation potential.
〔Ying, QL., et al.: Nature, 453, 519 (2008).]
Sodium Butyrate - 25 g
500 g
193-01522
197-01525
A histone deacetylase inhibitor. Promotes self-proliferation of embryonic stem cells.
〔Ware, CB., et al.: Cell Stem Cell, 4, 359 (2009).]
Thiazovivin for Cellbiology 1 mg
5 mg
202-18011
208-18013
Use with SB431542 and PD0325901 enhances reprogramming efficiency by over 200-fold and increases reprogramming speed.
〔Lin, T., et al.: Nat. Methods, 6, 805, (2009).]
Valproic Acid for Biochemistry 5 g
25 g
227-01071
225-01072
A histone deacetylase inhibitor. Can induce iPS cells from fibroblasts with transfer of only Oct4 and Sox2.
〔Huangfu, D., et al.: Nat. Biotechnol., 26, 1269 (2008).]
Y-27632 for Cellbiology 1 mg
5 mg
25 mg
257-00511
253-00513
251-00514
A ROCK inhibitor. Enhances post-cryopreservation survival and cloning efficiency of human embryonic stem cells and human iPS cells.

References:
  1. Kawamata, M., et al.: Proc. Natl. Acad. Sci. USA., 107, 14223 (2010).
  2. Claassen, DA., et al.: Mol. Reprod. Dev., 76, 722(2009).
  3. Martin-Ibanez, R., et al.: Hum. Reprod., 23, 2744(2008).
  4. Watanabe, K., et al.: Nat. Biotechnol., 25, 681(2007).
  5. Sakamoto, K., et al.: J. Pharamacol. Sci., 92, 56(2003).
  6. Nishimaru, K., et al.: J. Pharamacol. Sci., 92, 424(2003).
  7. Uehata, M., et al.: Nature, 389, 990 (1997).
* This product is marketed under the license of Mitsubishi Tanabe Pharma Corporation.
NEW! 5 mmol/L Y-27632 Solution for Cell Culture 300 μL 253-00591
Y-27632, diluted to 5 mmol/L with water.

QUOTATION REQUEST for reagents for ES/iPS Cell Research
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[ 2-b ]    Induction of differentiation and dedifferentiation, etc. of ES/iPS cells    ⇒ Leaflet
Product Name Grade Pkg. Size Wako Cat. #
A769662 for Cellbiology 5 mg 013-23551
An AMPK activator. Activation of AMPK inhibits cell growth, cell proliferation, and mTOR signaling.
AICAR [5-Amino-4-imidazolecarboxamide-1-β-D-ribofuranoside] for Cellbiology 100 mg
1 g
015-22531
011-22533
An AMPK activator. Suppresses differentiation into fat cells and induces differentiation of neural stem cells into astroglia.
  〔Giri, S., et al.: Nutr. Metab. (Lond), (2006).]
  〔Zang, Y., et al.: J. Biol. Chem., 283, 6201 (2008).]
Ciclosporin A for Cellbiology 50 mg
200 mg
031-18963
035-18961
Enhances the induction efficiency of mouse and human iPS cell differentiation into cardiac muscle and cardiac progenitor cells.
 〔Fujiwara, M., et al.: PLoS. One, 6, e16734 (2011).]
Ciglitazone for Cellbiology 5 mg 030-20981
A PPARγ agonist. Induces differentiation of human mesenchymal stem cells into fat cells and inhibits differentiation into osteoblasts.
  [Benvenuti, S., et al.: J. Endocrinol. Invest., 30, RC26 (2007).]
CKI-7 Dihydrochloride for Cellbiology 5 mg 034-21501
A casein kinase (CK1) inhibitor. Use with SB431542 and Y-27632 induces differentiation of human embryonic stem cells and human iPS cells into retinal progenitor cells in serum- and feeder cell-free culture medium.
  [Osakada, F., et al.: J. Cell Sci., 122, 3169 (2009).]
DAPT  [γ-Secretase Inhibitor IX] for Cellbiology 5 mg
25 mg
045-30981
041-30983
A γ-secretase inhibitor. Inhibits Notch signaling and induces differentiation of embryonic stem cells from embryoid body to neuron.
 〔Nelson, BR., et al.: Dev. Biol., 304, 479 (2007).]
 〔Crawford, TQ., et al.: Dev. Dyn., 236, 886 (2007).]
Dexamethasone
for Biochemistry 100 mg
1 g
047-18863
041-18861
Regulates bone differentiation of mesenchymal stem cells.
  [Jaiswal, N., et al.: J. Cell. Biochem., 64, 295 (1997).]
Dorsomorphin  [Compound C] for Cellbiology 1 mg
5 mg
045-31221
041-31223
>AMPK and BMP signal inhibitor. Induces differentiation of murine embryonic stem cells into cardiac muscle cells.
  [Hao, J., et al.: PLoS. One., 3, e2904 (2008).]
Dorsomorphin Dihydrochloride for Cellbiology 1 mg
5 mg
047-31801
043-31803
AMPK and BMP signal inhibitor. Induces differentiation of murine embryonic stem cells into cardiac muscle cells.
 〔Hao, J., et al.: PLoS. One., 3, e2904 (2008).]
Indolactam V
for Cellbiology 1 mg 097-05911
A protein kinase C activator. Induces differentiation of intestinal ectoderm into pancreatic progenitor cells during induction of differentiation of human embryonic stem cells into insulin-producing β-like cells.
 〔Melton, DA. et al.: Proc. Natl. Acad. Sci. USA., 106, 15768 (2009).]
IPA-3
for Cellbiology 5 mg 093-06231
A PAK inhibitor. Shows particularly high selectivity to PAK1. PAK1 is associated with tumor development and metastasis.
IWP-2 for Cellbiology 5 mg 090-06241
Activates porcupine (Porcn), a membrane-bound o-acyltransferase (MBOAT), and suppresses palmitylation of Wnt proteins, which in turn inhibits phosphorylation of Wnt-dependent Lrp6 receptor and accumulation of Dvl2 and β catenin.
IWR-1-endo for Cellbiology 5 mg 094-06381
A Wnt signaling inhibitor. Stabilizes β catenin-degradation complex (comprised of Axin2, Apc, Ck1, and Gsk3β) and stimulates degradation of β catenin.
LDN193189 Hydrochloride for Cellbiology 2 mg 124-06011
An inhibitor of BMP Type I receptor and ALK2/ALK3. BMP signaling regulates embryogenesis, and plays a physiologically crucial role in mature tissue.
    [Yu, P. B., et al.: Nat. Med., 14, 1363 (2008).]
LY-294002 for Biochemistry 5 mg
10 mg
25 mg
129-04861
125-04863
123-04864
A phosphatidylinositol 3-kinase inhibitor. Induces differentiation of murine embryonic stem cells into insulin-producing cells.
Purmorphamine for Cellbiology 5 mg 166-23991
A hedgehog signal agonist. Induces differentiation of mesenchymal progenitor cells and preosteoblastic cells into osteoblasts.
    [Wu, X., et al.: Chem. Biol., 11, 1229 (2004).]
all-trans-Retinoic Acid
for Biochemistry 50 mg
100 mg
250 mg
1 g
186-01114
182-01116
182-01111
188-01113
Induces differentiation into nerve cells.
Reversine for Cellbiology 5 mg 184-02391
A human A3 adenosine receptor agonist. Induces dedifferentiation of myoblasts into stem cells.
    [Chen, S., et al.: J. Am. Chem. Soc., 126, 410 (2004).]
NEW!    SB431542
for Cellbiology 5 mg
25 mg
192-16541
198-16543
An inhibitor of ALK4, ALK5, and ALK7. Stimulates proliferation,differentiation, and sheet formation of endothelial cells derived from embryonic stem cells. Use with thiazovivin and PD0325901 enhances reprogramming efficiency by over 200-fold and increases reprogramming speed.
    [Ogawa, K., et al.: J. Cell. Sci., 120, 55 (2007).]
    [Lin, T., et al.: Nat. Methods, 6, 805, (2009).]
Spermine for Biochemistry 250 mg
1 g
198-09811
194-09813
Induces differentiation into cardiac muscle cells.
    [Sasaki, T., et al.: Differentiation, 76, 1023 (2008).]
Trichostatin A for Cellbiology 1 mg
5 mg
203-17561
209-17563
A histone deacetylase inhibitor. Induces differentiation into cardiac muscle cells.
    [Oyama, T., et al.: J. Cell. Biol., 176, 329 (2007).]
Troglitazone for Pharmacology Research 5 mg
50 mg
207-17601
203-17603
A PPARγ agonist. Induces differentiation of human mesenchymal stem cells into fat cell and inhibits differentiation into osteoblasts.
 〔Benvenuti, S., et al.: J. Endocrinol. Invest., 30, RC26 (2007).]
TWS119   [GSK-3β Inhibitor XII ] for Cellbiology 1 mg
5 mg
206-17671
202-17673
A GSK-3β inhibitor. Induces differentiation of murine embryonic stem cells into nerve cells.

QUOTATION REQUEST for reagents for ES/iPS Cell Research
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[ 2-c ]   StemSure Series
StemSure Series is a group of products whose quality has been confirmed by culturing mouse ES D3 strain cells and performing a colony forming test and alkaline phosphatase staining in addition to the quality specifications for liquid media for general uses.

Quality tests are performed using D3 mouse ES cell line.
StemSure Mercaptoethanol-Monothioglycerol-Gelatin Solution
StemSure Freezing Medium
StemSure LIF, Mouse, recombinant, Solution

Product Name Grade Pkg. Size Wako Cat. No.
StemSure 10 mol/L 2-Mercaptoethanol Solution (x100) for Cell Culture 100 mL 198-15781
StemSure 50 mol/L Monothioglycerol Solution (x100)
Equivalent to 2ME (2ME alternative)
for Cell Culture 100 mL 195-15791
StemSure 0.1w/v% Gelatin Solution for Cell Culture 500 mL 190-15805
StemSure Freezing Medium
    (⇒ Not available for sales in the US.)
for Cell Culture 100 mL 195-16031
StemSure LIF, Mouse, recombinant, Solution for Cell Culture 106 units 199-16051


[ 2-d ]   LIF, Human, recombinant, Culture Supernatant
    Used for maintenance of the differentiation potential of murine embryonic stem cells. [LINK]
Product Name Grade Pkg. Size Wako Cat. No.
LIF, Human, recombinant, Culture Supernatant
Not available for sale in the US.
for Cell Culture 1 mL 129-05601
1 mL x 10 125-05603



[ 2-e ]   N2 Supplements with Transferrin
Product Name Grade Pkg. Size Wako Cat. No.
N2-Supplement with Transferrin (Holo) (x 100) for Cell Culture 5 mL 141-08941
N2-Supplement with Transferrin (Apo) (x 100) for Cell Culture 5 mL 141-09041



[ 2-f ]   Products for Cell Culture
We supply a wide selection from liquid culture media through balanced saline solutions, cell detachment/dissociation solutions, antibiotic solutions, and to extracellular matrices. [LINK]


[ 2-g ]   BAMBANKER
Serum-Free Cell Freezing Medium for ES/iPS Cell Research

Product Name Manufacturer Pkg. Size Wako Cat. No.
BAMBANKER for Cell Culture 120 mL x 1 bottle 302-14681
  20 mL x 5 bottles 306-14684



[ 2-h ]   Sumitomo Bakelite's Products Line
for Regenerative Medicine Research   ⇒ LINK
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Note

  • Listed products are intended for laboratory research use only, and not to be used for drug, food or human.
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