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FUJIFILMFUJIFILM Wako Pure Chemical Corporation Laboratory Chemicals
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To suppress adsorption of extracellular vesicles to laboratory tools
EV-Save™ Extracellular Vesicle Blocking Reagent

1. LINK

2. Features

✔ EV-Save™ strongly suppresses adsorption of extracellular vesicles in culture supernatant or after purification to laboratory tools
✔ You only need to add EV-Save™ to the sample

3. Precautions for use

• The effect of the product cannot be obtained when it is used for serum, plasma, or samples containing a lot of impurities.
• The product contains polymer. Do not use the product if the polymer may have effect on the subsequent processes of the experiment.

4. Adsorption of purified extracellular vesicles is suppressed

■Loss by adsorption at transfer is almost completely suppressed
■Stability during storage is improved

A sample containing purified extracellular vesicle 3 ng/μL was prepared from COLO201 cells, added to a tube, transferred to a new tube, and allow to stand still for 3 minutes. Reduction in extracellular vesicles was determined by PS Capture™ Exosome ELISA Kit (Anti Mouse IgG POD). Graph shows CD63 signal relative to that obtained before transfer (100%).

5. Decrease in extracellular vesicles during ultrafiltration is prevented

■Extracellular vesicles are not lost during ultrafiltration

Reduction in extracellular vesicles after ultrafiltration for concentration (Vivaspin20, 100K dalton) of human iPScell culture supernatant was evaluated using PS Capture™ Exosome ELISA Kit (Anti Mouse IgG POD). Graph shows CD63 signal relative to that obtained before ultrafiltration (100%).

6. Recovery of purified extracellular vesicles is improved

■Recovery by MagCapture™ Exosome Isolation Kit PS is improved

Extracellular vesicles were purified from 1 mL of TIG3 cell culture supernatant using MagCapture™ Exosome Isolation Kit PS, and extracellular vesicles were measured using PS Capture™ Exosome ELISA Kit (Anti Mouse IgG POD). Graph shows CD63 signal relative to that obtained from input (100%).

7. Use in cell experiments

No effect on proliferation or pluripotency of human iPS cells

To TBS solution containing human iPScells, EV-Save™ was added at a ratio of 1/100, and the resultant TBS solution was added to culture medium at a ratio of 1/5. Human iPScells were cultured on this medium for 3 days, and viable cell count was determined. Graph shows CD63 signal relative to that obtained from a sample without EV-Save™(100%).

8. Product List

Wako Cat. No Product Name Pkg. Size Strage Condition
058-09261 EV-Save™ Extracellular Vesicle Blocking Reagent 1 mL keep at - 20°C

9. Related Product Lists

Wako Cat. Code Product Name Pkg. Size Strage Condition
299-77603 MagCapture™ Exosome Isolation Kit PS 2 purifications* Keep at 2 - 10°C
293-77601 2 purifications* Keep at 2 - 10°C
297-79201 PS Capture™ Exosome ELISA Kit (Anti Mouse IgG POD) 96 tests Keep at 2 - 10°C

* Used Exosome Capture-immobilized beads can be recycled for "Repeated extraction of extracellular vesicles from the same sample" and "Purification of extracellular vesicles from culture supernatant sample of the same lot and body fluid sample of the same lot". Recycling is up to 4 times.

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